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Development and characterization of a monoclonal antibody against Taura syndrome virus

Journal of Fish Diseases, 2009
AbstractWe produced a panel of monoclonal antibodies (MAbs) from the fusion of Taura syndrome virus variants from Belize (TSV‐BZ) immunized BALB/cJ mouse spleen cells and non‐immunoglobulin secreting SP2/0 mouse myeloma cells. One antibody, 2C4, showed strong specificity and sensitivity for TSV in dot‐blot immunoassay and immunohistochemistry (IHC ...
I, Côté   +3 more
openaire   +2 more sources

Taura syndrome virus from Venezuela is a new genetic variant

Aquaculture, 2008
In early 2005, the Aquaculture Pathology Laboratory of the University of Arizona received samples of diseased shrimp, Penaeus vannamei, from a Taura syndrome (TS) outbreak in the Lake Maracaibo region of Venezuela. Histopathology and in situ hybridization (ISH) were performed and the results confirmed the presence of Taura syndrome virus (TSV).
I. Côté   +4 more
openaire   +1 more source

Quantitation of Taura syndrome virus by real-time RT-PCR with a TaqMan assay

Journal of Virological Methods, 2004
A real-time RT-PCR assay was developed using a TaqMan probe to detect and quantify Taura syndrome virus (TSV) in penaeid shrimp. A pair of RT-PCR primers, which amplify a 72 bp DNA fragment, and a TaqMan probe were selected from open reading frame 1 (ORF1) of the TSV genome. The primers and TaqMan probe used in this assay reacted with TSV isolates from
Kathy F J, Tang   +2 more
openaire   +2 more sources

Identification of Genes Involved in Taura Syndrome Virus Resistance in Litopenaeus Vannamei

Journal of Aquatic Animal Health, 2014
Abstract The goal of the present research was to identify the genes that are differentially expressed between two lineages of Pacific white shrimp Litopenaeus vannamei displaying different susceptibilities to Taura syndrome virus (TSV) and to understand the molecular pathways involved in resistance to the disease.
Boube, I.   +4 more
openaire   +2 more sources

Viral resistance in shrimp that express an antisense Taura syndrome virus coat protein gene

Antiviral Research, 2005
Taura syndrome virus (TSV) is a major cause of mortality and morbidity in shrimp, and has a profound economic impact on commercial U.S. shrimp farming. This paper describes the stable expression of an antisense Taura syndrome virus-coat protein (TSV-CP) gene construct in shrimp zygotes, via transfection using jetPEI reagent, over a period of at least ...
Yuanan, Lu, Piera S, Sun
openaire   +2 more sources

Characterization of a Taura syndrome virus isolate originating from the 2004 Texas epizootic in cultured shrimp

Archives of Virology, 2010
A comprehensive investigation of the Taura syndrome virus (TSV) isolate that caused epizootics in shrimp farms in Texas in 2004 (Texas isolate) revealed that this virus was more virulent in laboratory bioassays than the TSV reference isolate, Hawaii 1994, causing severe symptom development and rapid mortality.
Arun K, Dhar   +7 more
openaire   +2 more sources

Turbidity detection of shrimp Taura Syndrome Virus by loop-mediated isothermal amplification reaction

2010 3rd International Nanoelectronics Conference (INEC), 2010
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a novel method of gene amplification that amplifies nucleic acid, which can be applied for disease diagnosis in shrimp aquaculture. During the LAMP reaction, the white precipitate of magnesium pyrophosphate (Mg 2 P 2 O 7 ) is formed correlates with the amount of synthesized
A. Sappat   +4 more
openaire   +1 more source

Demonstration of Infectious Taura Syndrome Virus in the Feces of Seagulls Collected during an Epizootic in Texas

Journal of Aquatic Animal Health, 1997
Abstract Laughing gulls Larus atricilla were observed feeding on diseased shrimp Penaeus vannamei during an epizootic of the Taura syndrome virus (TSV) at a south Texas farm in May 1995. Fresh fecal samples were collected from a gull roosting site on a pond levee where gulls that fed on diseased shrimp had roosted overnight.
J. R. Garza   +5 more
openaire   +1 more source

Nucleotide sequence of 3′-end of the genome of Taura syndrome virus of shrimp suggests that it is related to insect picornaviruses

Archives of Virology, 2001
Taura syndrome disease, caused by Taura syndrome virus (TSV), is one of the most important viral diseases of penaeid shrimp in the Western Hemisphere resulting in catastrophic disease epidemics in farmed shrimp. We have cloned and sequenced a 3278 bp cDNA representing the 3' end of the TSV genome.
R, Robles-Sikisaka   +3 more
openaire   +2 more sources

In vitro cultivation of shrimp Taura syndrome virus (TSV) in a C6/36 mosquito cell line

Journal of Fish Diseases, 2011
Journal of Fish Diseases Volume 34, Issue 10, pages 805–810, October 2011 Additional Information How to Cite Arunrut, N., Phromjai, J., Gangnonngiw, W., Kanthong, N., Sriurairatana, S. and Kiatpathomchai, W. (2011), In vitro cultivation of shrimp Taura syndrome virus (TSV) in a C6/36 mosquito cell line. Journal of Fish Diseases, 34: 805– 810.
N, Arunrut   +5 more
openaire   +2 more sources

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