Results 51 to 60 of about 31,972 (221)

Calpain inhibition stimulates caspase-dependent apoptosis induced by taxol in NIH3T3 cells

, 2007
Taxol is an anticancer drug that triggers apoptosis in a wide spectrum of cancers such as ovarian, breast, lung, head and neck, and bladder carcinoma by both caspase-dependent and -independent apoptosis mechanisms.
Salinas, Matilde, González, Victor, Martín, Elena, Guerra Pérez, Natalia, Piñeiro, David   +4 more
core   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Production and bioprocessing of Taxol from Aspergillus niger, an endophyte of Encephalartos whitelockii, with a plausible biosynthetic stability: antiproliferative activity and cell cycle analysis

Microbial Cell Factories
The biosynthetic potency of Taxol by fungi raises their prospective to be a platform for commercial production of Taxol, nevertheless, the attenuation of its productivity with the fungal storage, is the challenge.
Asmaa Gamal, Eman Fikry, Nora Tawfeek, Azza M. El-Shafae, Ashraf S. A. El-Sayed, Maher M. El-Domiaty   +5 more
doaj   +1 more source

Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation

Scientific Reports, 2017
Taxol, a first-line anti-tumour drug, has low effectiveness against colorectal cancer. Combination with other agents is an effective strategy to enhance Taxol cytotoxicity.
Yijia Wang, Chunze Zhang, Shiwu Zhang, Zhenying Zhao, Jiawen Wang, Jiali Song, Yue Wang, Jun Liu, Shaobin Hou   +8 more
doaj   +1 more source

Taxol crystals can masquerade as stabilized microtubules. [PDF]

PLoS ONE, 2008
Taxol is a potent anti-mitotic drug used in chemotherapy, angioplastic stents, and cell biology research. By binding and stabilizing microtubules, Taxol inhibits their dynamics, crucial for cell division, motility, and survival.
Margit Foss, Buck W L Wilcox, G Bradley Alsop, Dahong Zhang   +3 more
doaj   +1 more source

Titin Is Present in the Elastic Tethers That Connect Separating Anaphase Chromosomes in Crane‐Fly Spermatocytes

Cytoskeleton, EarlyView.
ABSTRACT Elastic tethers connect telomeres of separating chromosomes in anaphase of animal cells. Immunofluorescence staining of titin in crane‐fly spermatocytes, using 4 different antibodies, shows that the giant elastic protein titin seems to be a component of mitotic tethers: titin “strands” extend between separating chromosomes, connecting their ...
Demetra Economopoulos, Maral Janan, Martina Krüger, Aavo‐Valdur Mikelsaar, Arthur Forer, Rose Sheykhani   +5 more
wiley   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

Visualizing Specific Tubulin Isotypes and Pathogenic Variants in Cellular Microtubule Arrays

Cytoskeleton, EarlyView.
ABSTRACT Eukaryotic cells depend on dynamic microtubule arrays to execute a wide range of functions vital for life. These microtubule filaments are formed through the polymerization of α/β‐tubulin proteins, which can be generated from numerous tubulin genes, or isotypes.
Sareen Fiaz, Benjamin Powers, Jayne Aiken   +2 more
wiley   +1 more source

Antioxidant and Cytotoxic Properties of Piper spp. Volatile Oils That Inhibit Pathogenic Microorganisms

Flavour and Fragrance Journal, EarlyView.
The samples were fungicidal against Candida, with MICs ranging from 62.5 to 250 μg/mL, and fungistatic against Penicillium adametzii, Aspergillus flavus and A. niger, with MICs of 1000 μg/mL. The volatile oils of Piper aduncum and Piper marginatum reduced the viability of MDA‐MB231 cells.
Midiã R. Oliveira, Ely D. Alfaia Neto, Carlos Eduardo C. Alves, Renata Galvão de Azevedo, Alice F. Gomes, Flávia Juliana Reis de Sousa, Juan Campos de Oliveira, Larissa Raquel S. Maciel, Gemilson S. Pontes, Ari F. Hidalgo, Antonia Q. L. Souza, Rita C. Saraiva Nunomura   +11 more
wiley   +1 more source

Translation regulation after taxol treatment in NIH3T3 cells involves the elongation factor (eEF)2

, 2007
Changes to the translational machinery that occur during apoptosis have been described in the last few years. The two principal ways in which translational factors are modified during apoptosis are: (i) changes in protein phosphorylation and (ii ...
González, Víctor M, Salinas, Matilde, Hernández Jiménez, Macarena, Macarena Hernández-Jiménez, David Piñeiro, M. Elena Martín, Víctor M. González, Matilde Salinas, Martín, M. Elena, Piñeiro, David   +9 more
core   +1 more source