Results 71 to 80 of about 7,420,775 (255)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Modern possibilities of treating brain tumors: intraoperative technologies in neurooncology

Клиническая практика
The fundamental principle of neurooncology is the maximal removal of the tumor, simultaneously minimizing the effects on the healthy brain structures.
Arthur I. Nafikov, Radmir I. Minnigaleev, Elina R. Yarullina, Eminat M. Magomedova, Karina G. Soboleva, Maksim M. Sobolev, Oishakhon R. Egamova, Yulia A. Kirillova, Arut A. Arutyunyan, Fatimat A. Tokhova, Nurlana A. Abbasova, Khanipat Kh. Saadueva, Ilshat F. Yarmeev   +12 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Advances in Recycling Technologies of Critical Metals and Resources from Cathodes and Anodes in Spent Lithium-Ion Batteries

Separations
With the rapid economic development and the continuous growth in the demand for new energy vehicles and energy storage systems, a significant number of waste lithium-ion batteries are expected to enter the market in the future.
Shuwen Wang, Yanrong Lai, Jingran Yang, Jiaxue Zhao, Yushan Zhang, Miaoling Chen, Jinfeng Tang, Junhua Xu, Minhua Su   +8 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Towards Equitable Representations of Ageing: Evaluation of Gender, Territories, Aids and Artificial Intelligence

Land
There are few studies on the representation of older people regarding aids and assistive devices and even fewer that incorporate more inclusive views (gender, emotions, anti-ageist, territorial or land approach) as well as virtual or land ethnography or ...
Vanessa Zorrilla-Muñoz, Daniela Luz Moyano, Carolina Marcos Carvajal, María Silveria Agulló-Tomás   +3 more
doaj   +1 more source

Technologies may help thinking [PDF]

, 2006
The objective of teachers’ personal and professional development is an excellent reason to reflect upon the innovation issues in education and a rare opportunity to implement the use of portfolios in the teaching practices.
Costa, Fernando Albuquerque, Cruz, Elisabete, Ferreira, Rute   +2 more
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