Results 91 to 100 of about 1,016,447 (267)

Nonsignificant elevational trends of soil microbial respiration and temperature sensitivity in a subtropical forest

Ecosphere
Soil carbon (C) cycling plays a critical role in regulating global C budget and atmospheric CO2 concentrations. The ongoing global warming potentially accelerates soil C loss induced by microbial respiration (MR) and makes soil a large C source to the ...
Bingwei Zhang, Yun Jiang, Xianjin He, Youshi Wang, Jiawei Li, Xueli Huang, Zufei Shu, Buhang Li, Chengjin Chu   +8 more
doaj   +1 more source

Phosphatidylinositol-4,5-biphosphate (PIP₂)-Dependent Thermoring Basis for Cold-Sensing of the Transient Receptor Potential Melastatin-8 (TRPM8) Biothermometer

Physchem
The menthol sensor transient receptor potential melastatin-8 (TRPM8) can be activated by cold and, thus, serves as a biothermometer in a primary afferent sensory neuron for innocuous-to-noxious cold detection.
Guangyu Wang
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Thermal Evaluation of the Initial Concept 3.X Vehicle at Mach 7

Energies
High-speed global surface temperature distributions and heat flux measurements on the Initial Concept 3.X vehicle (IC3X) model were investigated at the UTSA Mach 7 wind tunnel, examining angles of attack of 0° and 5° at a freestream unit Reynolds number (
Abinayaa Dhanagopal, Nathan S. Strasser, Angelina Andrade, Kevin R. Posladek, Eugene N. A. Hoffman, Christopher S. Combs   +5 more
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Causal hybrid modeling with double machine learning—applications in carbon flux modeling

Machine Learning: Science and Technology
Hybrid modeling integrates machine learning with scientific knowledge to enhance interpretability, generalization, and adherence to natural laws. Nevertheless, equifinality and regularization biases pose challenges in hybrid modeling to achieve these ...
Kai-Hendrik Cohrs, Gherardo Varando, Nuno Carvalhais, Markus Reichstein, Gustau Camps-Valls   +4 more
doaj   +1 more source

Evaluation and application of low damage guar gum fracturing fluid in deep coalbed methane reservoirs

Meikuang Anquan
An evaluation of low damage guar gum fracturing fluid system based on deep coalbed methane reservoir stimulation was carried out, its on-site application research was conducted.
Yongcheng ZHANG, Liangliang LIU, Dehui LI, Yang BAI, Wei ZHANG, Yuxia QIN   +5 more
doaj   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source