Results 51 to 60 of about 219,031 (288)

Genetic diversity and differentiation of Pinus sylvestris L. from the IUFRO 1982 provenance trial revealed by AFLP analysis [PDF]

Archives of Biological Sciences, 2015
DNA markers have become effective tools in genetic diversity studies of forest trees. However, molecular marker analyses are associated with laborious and costly effort. One of the possibilities to overcome these constraints is to analyze bulked
Androsiuk Piotr, Ciaglo-Androsiuk Sylwia, Urbaniak Lech   +2 more
doaj   +1 more source

Hox Genes Regulate Muscle Founder Cell Pattern Autonomously and Regulate Morphogenesis Through Motor Neurons [PDF]

, 2010
The differentiation of myoblasts to form functional muscle fibers is a consequence of interactions between the mesoderm and ectoderm. The authors examine the role of segment identity in directing these interactions by studying the role of Hox genes in ...
Dutta, Devkanya, Lewis, Edward. B., Rodrigues, Veronica, Umashankar, M., VijayRaghavan, K.   +4 more
core   +1 more source

Genetically Engineered Organization: Protein Template, Biological Recognition Sites, and Nanoparticles

Advanced Materials Interfaces, 2016
Nanoparticles and their assemblies exhibit properties that can be used for a wide range of applications. However, creating multifunctional assemblies has remained challenging. Inspired by magnetotactic bacteria, genetically engineered single building blocks from magnetosome chains are used and complemented by additional components to form fluorescent ...
Jehle, F., Valverde-Tercedor, C., Reichel, V., Carillo, M., Bennet, M., Günther, E., Wirth, R., Mickoleit, F., Zarivach, R., Schüler, D., Blank, K., Faivre, D.   +11 more
openaire   +5 more sources

Pathogenic Germline PALB2 and RAD50 Variants in Patients With Relapsed Ewing Sarcoma

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Approximately 10% of patients with Ewing sarcoma (EwS) have pathogenic germline variants. Here, we report two cases: first, a novel germline pathogenic variant in partner and localizer of BRCA2 (PALB2) in a patient with a late EwS relapse. Its impact on homologous recombination is demonstrated, and breast cancer risk is discussed.
Molly Mack, A. Carolina Tufino, Mohd Azrin Jamalruddin, Sreya Dey, Yinghong Pan, Elina Mukherjee, Jessica D. Daley, Julia Meade, Mahmoud Aarabi, Lisa Maurer, James Cooper, Kara A. Bernstein, Kelly M. Bailey   +12 more
wiley   +1 more source

Site-Specific Incorporation of Functional Components into RNA by an Unnatural Base Pair Transcription System

Molecules, 2012
Toward the expansion of the genetic alphabet, an unnatural base pair between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and pyrrole-2-carbaldehyde (Pa) functions as a third base pair in replication and transcription, and provides a useful tool for the site-
Rie Kawai, Nobuyuki Morohashi, Michiko Kimoto, Akira Sato, Ichiro Hirao   +4 more
doaj   +1 more source

Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination

BMC Biotechnology, 2020
Background Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand breaks (DSB) through homology directed repair (HDR).
Sanum Bashir, Tu Dang, Jana Rossius, Johanna Wolf, Ralf Kühn   +4 more
doaj   +1 more source

The search for spinning black hole binaries in mock LISA data using a genetic algorithm

, 2010
Coalescing massive Black Hole binaries are the strongest and probably the most important gravitational wave sources in the LISA band. The spin and orbital precessions bring complexity in the waveform and make the likelihood surface richer in structure as
Babak, Stanislav, Feroz, Farhan, Petiteau, Antoine, Shang, Yu   +3 more
core   +3 more sources

Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling. [PDF]

, 2020
The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of ...
Aris, Kevin D Palacio, Bryant, Zev, Cofsky, Joshua C, Doudna, Jennifer A, Ivanov, Ivan E, Wright, Addison V   +5 more
core   +3 more sources

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source