Results 61 to 70 of about 3,144,500 (368)

Site-Specific Incorporation of Functional Components into RNA by an Unnatural Base Pair Transcription System

Molecules, 2012
Toward the expansion of the genetic alphabet, an unnatural base pair between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and pyrrole-2-carbaldehyde (Pa) functions as a third base pair in replication and transcription, and provides a useful tool for the site-
Rie Kawai, Nobuyuki Morohashi, Michiko Kimoto, Akira Sato, Ichiro Hirao   +4 more
doaj   +1 more source

Genetic diversity and differentiation of Pinus sylvestris L. from the IUFRO 1982 provenance trial revealed by AFLP analysis [PDF]

Archives of Biological Sciences, 2015
DNA markers have become effective tools in genetic diversity studies of forest trees. However, molecular marker analyses are associated with laborious and costly effort. One of the possibilities to overcome these constraints is to analyze bulked
Androsiuk Piotr, Ciaglo-Androsiuk Sylwia, Urbaniak Lech   +2 more
doaj   +1 more source

Impact of template backbone heterogeneity on RNA polymerase II transcription. [PDF]

, 2014
Variations in the sugar component (ribose or deoxyribose) and the nature of the phosphodiester linkage (3'-5' or 2'-5' orientation) have been a challenge for genetic information transfer from the very beginning of evolution.
Chong, Jenny, Huang, Xuhui, Wang, Dong, Wang, Wei, Xu, Liang, Zhang, Lu   +5 more
core   +1 more source

Evaluation of the template letter regarding the disclosure of genetic information within the family in France [PDF]

Journal of Community Genetics, 2019
The 2011 French Bioethics Law regarding disclosure of genetic information within families enables health professionals to notify any at-risk relatives directly, with the patient's consent, using a template letter. To assess the impact of this template letter in terms of understanding, personal feelings and intent to contact a health professional, we ...
Emmanuelle Haquet, Pauline Roche, Didier Lacombe, Didier Lacombe, Nicolas Taris, Linda Akloul, Virginie Dorian, Cécile Zordan, Laura Richert, Edouard Lhomme, Eva Toussaint, Christophe Cordier, Laetitia Monteil, Aline Maillard, Laurent Pasquier   +14 more
openaire   +4 more sources

Search-and-replace genome editing without double-strand breaks or donor DNA

Nature, 2019
Most genetic variants that contribute to disease1 are challenging to correct efficiently and without excess byproducts2–5. Here we describe prime editing, a versatile and precise genome editing method that directly writes new genetic information into a ...
Andrew V. Anzalone, Peyton B. Randolph, Jessie R. Davis, Alexander A. Sousa, Luke W. Koblan, Jonathan M. Levy, Peter J. Chen, Christopher Wilson, Gregory A. Newby, Aditya Raguram, David R. Liu   +10 more
semanticscholar   +1 more source

Eye tracking by genetic deformable template matching algorithm in video sequences [PDF]

Port-Said Engineering Research Journal, 2013
Eye detection and tracking has broad applications in human-computer interaction, behavioural analysis, computer graphics, security, and biology systems. In this paper, we propose innovative eye detection and tracking method in video sequences. The proposed method incorporates deformable template matching and genetic algorithm to overcome problems ...
Rehab Farouk, Sheren Zakaria, Randa Atta
openaire   +2 more sources

Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry

Nature, 2008
DNA sequence information underpins genetic research, enabling discoveries of important biological or medical benefit. Sequencing projects have traditionally used long (400–800 base pair) reads, but the existence of reference sequences for the human and ...
D. Bentley, S. Balasubramanian, H. Swerdlow, G. P. Smith, J. Milton, Clive Brown, K. Hall, D. Evers, Colin L. Barnes, Helen Bignell, J. M. Boutell, J. Bryant, Richard J. Carter, R. K. Cheetham, A. Cox, D. Ellis, Michael R. Flatbush, N. Gormley, S. Humphray, Leslie J. Irving, Mirian Karbelashvili, Scott M. Kirk, Heng Li, Xiaohai Liu, K. Maisinger, L. Murray, B. Obradovic, T. Ost, Michael L. Parkinson, Mark Pratt, I. Rasolonjatovo, M. Reed, R. Rigatti, C. Rodighiero, M. Ross, A. Sabot, S. Sankar, A. Scally, G. Schroth, Mark E. B. Smith, Vincent P. Smith, Anastassia Spiridou, Peta E. Torrance, Svilen Tzonev, Eric H. Vermaas, Klaudia Walter, Xiaolin Wu, Lu Zhang, Mohammed D. Alam, C. Anastasi, Ify C. Aniebo, D. M. Bailey, Iain Bancarz, Saibal Banerjee, Selena Barbour, P. Baybayan, Vincent Benoit, Kevin Benson, Claire Bevis, Phillip J. Black, Asha Boodhun, J. S. Brennan, J. Bridgham, Rob C. Brown, A. Brown, Dale H. Buermann, Abass A. Bundu, James C. Burrows, N. Carter, Nestor Castillo, M. C. E. Catenazzi, Simon Chang, R. Cooley, Natasha R. Crake, Olubunmi O. Dada, Konstantinos D. Diakoumakos, Belen Dominguez-Fernandez, D. Earnshaw, Ugonna C. Egbujor, Dave Elmore, S. Etchin, Mark Ewan, M. Fedurco, Louise J. Fraser, K. F. Fajardo, W. S. Furey, Dave George, Kimberley J. Gietzen, Colin P. Goddard, G. Golda, Philip A. Granieri, David E. Green, D. Gustafson, Nancy F. Hansen, K. Harnish, C. Haudenschild, Narinder I. Heyer, Matthew M. Hims, Johnny T. Ho, Adrian Horgan, Katya Hoschler, Steve Hurwitz, D. V. Ivanov, Maria Q. Johnson, Terena James, T. A. Jones, Gyoung-Dong Kang, Tzvetana H. Kerelska, A. Kersey, I. Khrebtukova, A. Kindwall, Z. Kingsbury, P. Kokko-Gonzales, Anil Kumar, M. Laurent, C. Lawley, Sarah E. Lee, X. Lee, A. Liao, Jennifer A. Loch, Mitch Lok, Shujun J Luo, Radhika M. Mammen, J. W. Martin, P. McCauley, P. McNitt, Parul Mehta, Keith Moon, Joe W. Mullens, T. Newington, Z. Ning, B. Ng, Sonia M. Novo, Michael J. O'neill, M. Osborne, A. Osnowski, Omead Ostadan, L. Paraschos, L. Pickering, Andrew C. Pike, A. Pike, D. Chris Pinkard, Daniel P. Pliskin, Joe Podhasky, Victor J. Quijano, C. Raczy, Vicki H. Rae, S. Rawlings, Ana Chiva Rodriguez, Phyllida Roe, J. Rogers, M. C. Rogert Bacigalupo, Nikolai Romanov, A. Romieu, Rithy K. Roth, Natalie J. Rourke, Silke T. Ruediger, E. Rusman, Raquel M. Sanches-Kuiper, M. Schenker, J. Seoane, Richard Shaw, Mitch Shiver, S. Short, N. Sizto, Johannes P. Sluis, M. A. Smith, J. S. Sohna, Eric Spence, K. Stevens, Neil W. Sutton, L. Szajkowski, C. Tregidgo, G. Turcatti, S. Vandevondele, Yuli Verhovsky, Selene M. Virk, S. Wakelin, Gregory Walcott, Jingwen Wang, G. Worsley, Juying Yan, L. Yau, Mike Zuerlein, J. Rogers, J. Mullikin, M. Hurles, N. J. McCooke, John S. West, F. Oaks, Peter L. Lundberg, D. Klenerman, R. Durbin, Anthony J. Smith   +193 more
semanticscholar   +1 more source

Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination

BMC Biotechnology, 2020
Background Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand breaks (DSB) through homology directed repair (HDR).
Sanum Bashir, Tu Dang, Jana Rossius, Johanna Wolf, Ralf Kühn   +4 more
doaj   +1 more source

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

Rapid Hypothesis Testing in Candida albicans Clinical Isolates Using a Cloning-Free, Modular, and Recyclable System for CRISPR-Cas9 Mediated Mutant and Revertant Construction

Microbiology Spectrum, 2022
As increasing evidence emerges that interstrain genetic diversity among Candida albicans clinical isolates underpins phenotypic variation compared to the reference isolate SC5314, new genetic tools are required to interrogate gene function across strain ...
Junyan Liu, Amanda K. Vogel, Jian Miao, Jennifer A. Carnahan, David J. Lowes, Jeffrey M. Rybak, Brian M. Peters   +6 more
doaj   +1 more source