Results 61 to 70 of about 219,031 (288)
Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]
, 2014 Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R +3 more
core +1 more source
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
FEBS Letters, EarlyView.The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source
Drivers of within-host genetic diversity in acute infections of viruses.
PLoS Pathogens, 2020 Genetic diversity is the fuel of evolution and facilitates adaptation to novel environments. However, our understanding of what drives differences in the genetic diversity during the early stages of viral infection is somewhat limited. Here, we use ultra-
Maoz Gelbart +8 more
doaj +1 more source
Genetic Programming for Multibiometrics
, 2012 Biometric systems suffer from some drawbacks: a biometric system can provide in general good performances except with some individuals as its performance depends highly on the quality of the capture. One solution to solve some of these problems is to use
Giot, Romain, Rosenberger, Christophe
core +3 more sources
The role of histone modifications in transcription regulation upon DNA damage
FEBS Letters, EarlyView.This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley +1 more source
Microbiology Spectrum, 2022 As increasing evidence emerges that interstrain genetic diversity among Candida albicans clinical isolates underpins phenotypic variation compared to the reference isolate SC5314, new genetic tools are required to interrogate gene function across strain ...
Junyan Liu +6 more
doaj +1 more source
ML Estimation of DNA Initial Copy Number in Polymerase Chain Reaction (PCR) Processes [PDF]
, 2007 Estimation of DNA copy number in a given biological sample is an extremely important problem in genomics. This problem is especially challenging when the number of the DNA strands is minuscule, which is often the case in applications such as pathogen and
Hassibi, A., Hassibi, B., Vikalo, H.
core +1 more source
FEBS Letters, EarlyView.Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley +1 more source
Effective Use of Linear DNA in Cell-Free Expression Systems
Frontiers in Bioengineering and Biotechnology, 2021 Cell-free expression systems (CFEs) are cutting-edge research tools used in the investigation of biological phenomena and the engineering of novel biotechnologies. While CFEs have many benefits over in vivo protein synthesis, one particularly significant
Megan A. McSweeney, Mark P. Styczynski
doaj +1 more source
Experiments in Linear Template Combination using Genetic Algorithms
, 2016 6 ...
Bhatnagar, Nikhilesh, Mamidi, Radhika
openaire +2 more sources