Results 41 to 50 of about 70,210 (273)

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Klein, Hanns-Georg, Biskup, S., Vogl, Ina, Hirv, K., Greif, P.A., Kotschote, S., Gehring, A., Kuhn, M., Stuhrmann, Manfred, Bergmann, C., Eck, Sebastian H., Biskup, Saskia, Metzeler, Klaus H., Greif, Philipp A., Stuhrmann, M., Bolz, Hanno Joern, Benet-Pages, Anna, Hirv, Kaimo, Benet-Pagès, A., Klein, H.G., Vogl, I., Bolz, H.J., Kuhn, Marius, Kotschote, Stefan, Gehring, Andrea, Bergmann, Carsten, Metzeler, K.H., Eck, S.H.   +27 more
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Comparative analysis of human DNA extraction methods and mitochondrial DNA HV1 and HV2 haplogroup determination [PDF]

Kragujevac Journal of Science, 2020
The high integrity of extracted DNA is necessary for the determination of human haplogroups, based on the mitochondrial DNA (mtDNA) marker sequences. The study aims were to isolate the total DNA from selected human samples and establish a protocol that ...
Nikezić Aleksandra G., Blagojević Stefan Z., Ćupurdija Maja Đ., Planojević Nevena S., Jovankić Jovana V., Rakobradović Jelena D., Vidanović Dejan S., Arsenijević Dejan D., Marković Snežana D.   +8 more
doaj  

Structural variation in the chicken genome identified by paired-end next-generation DNA sequencing of reduced representation libraries [PDF]

, 2011
Background Variation within individual genomes ranges from single nucleotide polymorphisms (SNPs) to kilobase, and even megabase, sized structural variants (SVs), such as deletions, insertions, inversions, and more complex rearrangements.
Kerstens Hindrik HD, Vereijken, A., Martien AM Groenen, Bert W Dibbits, Groenen, M.A.M., Dibbits, B.W., Kerstens, H.H.D., Dibbits Bert W, Okimoto Ron, Hindrik HD Kerstens, Richard PMA Crooijmans, Addie Vereijken, Vereijken Addie, Crooijmans, R.P.M.A., Okimoto, R., Groenen Martien AM, Crooijmans Richard PMA, Ron Okimoto   +17 more
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An Alternative Platform for Protein Expression Using an Innate Whole Expression Module from Metagenomic DNA

Microorganisms, 2019
Many integrated gene clusters beyond a single genetic element are commonly trapped as the result of promoter traps in (meta)genomic DNA libraries. Generally, a single element, which is mainly the promoter, is deduced from the resulting gene clusters and ...
Dae-Eun Cheong, So-Youn Park, Ho-Dong Lim, Geun-Joong Kim   +3 more
doaj   +1 more source

Genetic variation in N- and C-terminal regions of bovine DNAJA1 heat shock protein gene in African, Asian and American cattle [PDF]

Journal of Genomics, 2018
DNAJA1 or heat shock protein 40 (Hsp40) is associated with heat adaptation in various organisms. We amplified and sequenced a total of 1,142 bp of bovine Hsp40 gene representing the critical N-terminal (NTR) and C-terminal (CTR) regions in representative samples of African, Asian and American cattle breeds.
Ajayi, Oyeyemi O., Peters, Sunday O., De Donato, Marcos, Mujibi, F. Denis, Khan, Waqas A., Hussain, Tanveer, Babar, Masroor E., Imumorin, Ikhide G., Thomas, Bolaji N.   +8 more
openaire   +2 more sources

Genetic polymorphism and natural selection in the C-terminal 42 kDa region of merozoite surface protein-1 among Plasmodium vivax Korean isolates [PDF]

Malaria Journal, 2012
AbstractBackgroundThe carboxy-terminal 42 kDa region ofPlasmodium vivaxmerozoite surface protein-1 (PvMSP-142) is a leading candidate antigen for blood stage vaccine development. However, this region has been observed to be highly polymorphic among filed isolates ofP. vivax. Therefore it is important to analyse the existing diversity of this antigen in
Kang, Jung-Mi, Ju, Hye-Lim, Kang, Yoo-Mi, Lee, Dong-Hyun, Moon, Sung-Ung, Sohn, Woon-Mok, Park, Jae-Won, Kim, Tong-Soo, Na, Byoung-Kuk   +8 more
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A functional genetic screen identifies regions at the C-terminal tail and death-domain of death-associated protein kinase that are critical for its proapoptotic activity [PDF]

Proceedings of the National Academy of Sciences, 2000
Death-associated protein kinase (DAP-kinase) is a Ca +2 /calmodulin-regulated serine/threonine kinase with a multidomain structure that participates in apoptosis induced by a variety of signals. To identify regions in this protein that are critical for its proapoptotic activity, we performed a genetic screen on the ...
T, Raveh, H, Berissi, M, Eisenstein, T, Spivak, A, Kimchi   +4 more
openaire   +2 more sources

Comparative genome analysis provides insights into the evolution and adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum. [PDF]

, 2010
A recently emerging bleeding canker disease, caused by Pseudomonas syringae pathovar aesculi (Pae), is threatening European horse chestnut in northwest Europe. Very little is known about the origin and biology of this new disease.
Green, S., Richard Thwaites, Cottrell, Joan E, Jackson, Robert W., Studholme, David J., David J Studholme, Lovell, H., Jackson, R. W., Green, S, Dorati, F, Lovell, H, Kamoun, S., Bridget E Laue, Jackson, RW, Bridgett, S, Lovell, Helen, Robert W Jackson, Huitema, Edgar; id_orcid, Sharp, PM, Arnold, Dawn L., Blaxter, M., Thwaites, R, Paul M Sharp, Huitema, E., Studholme, DJ, Cottrell, J.E., Edgar Huitema, Sharp, Paul M, Laue, Bridget E, Thwaites, R., Bridgett, Stephen, Huitema, E, Sophien Kamoun, Studholme, D. J., Thwaites, Richard, Blaxter, M, Studholme, D.J., Federico Dorati, Arnold, D., Jackson, R., Laue, B.E., Laue, B. E., Joan E Cottrell, Sharpe, P.M., Cottrell, J. E., Bridgett, S., Kamoun, Sophien, Dorati, Federico, Sharp, Paul M., Dorati, F., Arnold, D, Huitema, Edgar, Arnold, Dawn, Helen Lovell, Dawn Arnold, Sarah Green, Cottrell, Joan E., Blaxter, Mark, Mark Blaxter, Laue, Bridget E., Cottrell, JE, Jackson, Robert W, Green, Sarah, Sharp, P. M., Stephen Bridgett, Laue, BE, Kamoun, S, Studholme, David J   +67 more
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Architecture of the single-plasmid three-component genetic circuit used to evaluate scDBD repressor constructs.

, 2023
Plasmid architecture (A) includes the ColE1 origin and ampicillin resistance selection marker (AmpR) in addition to the three genetic circuit components: pDBD(scDBD), pGFP(eGFP), and pLacI(LacIW220F), incorporated at Cloning Sites I, II, and III ...
James A. Davey (1433212), Natalie K. Goto (1746604)   +1 more
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Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene [PDF]

Journal of Bacteriology, 1993
The nucleotide sequence of the C-terminal region of the recE gene of the Rac prophage of Escherichia coli K-12 reveals the presence of a partially overlapping reading frame we call recT. Deletion mutations show that recT is required for the RecE pathway of conjugational recombination.
A J, Clark, V, Sharma, S, Brenowitz, C C, Chu, S, Sandler, L, Satin, A, Templin, I, Berger, A, Cohen   +8 more
openaire   +2 more sources