Results 1 to 10 of about 724 (141)

Transcriptomic analysis reveals pathways underlying the multi‐antibiotic resistance of Klebsiella pneumoniae [PDF]

IET Systems Biology
Klebsiella pneumoniae, an opportunistic pathogen, is pervasively distributed across the world. Its escalating antibiotic resistance poses a serious threat to global public health. The mechanisms behind this resistance remain largely elusive.
Ying Liu, Zhihui Niu, Rile Wu, Dezhi Yang, Jun Chen, Guoqing Liu, Jun Zhao   +6 more
doaj   +2 more sources

Formylation of tetrahydrofolate by formyl phosphate [PDF]

FEBS Letters, 1990
Formyl phosphate is the putative intermediate in the formylation of tetrahydrofolate (THF) catalyzed by N10‐formylTHF synthetase. In this study the non‐enzymic reaction between formyl phosphate and THF was examined at 5°C. 1H‐NMR, HPLC and kinetic analysis of the proton‐catalyzed conversion of the product to N5,10‐methenylTHF were used to identify the ...
Jahansouz, Hossain, Scherübel, Devin M., Himes, Richard H.   +2 more
openaire   +2 more sources

Application of a Colorimetric Assay to Identify Putative Ribofuranosylaminobenzene 5'-Phosphate Synthase Genes Expressed with Activity in Escherichia coli

Biological Procedures Online, 2003
Tetrahydromethanopterin (H4MPT) is a tetrahydrofolate analog originally discovered in methanogenic archaea, but later found in other archaea and bacteria. The extent to which H4MPT occurs among living organisms is unknown.
Bechard Matthew E., Chhatwal Sonya, Garcia Rosemarie E., Rasche Madeline E.   +3 more
doaj   +1 more source

Mthfs is an essential gene in mice and a component of the purinosome

Frontiers in Genetics, 2011
Tetrahydrofolates (THF) are a family of cofactors that function as one-carbon donors in folate-dependent one-carbon metabolism, a metabolic network required for the de novo synthesis of purines, thymidylate, and for the remethylation of homocysteine to ...
Martha S Field, Donald D Anderson, Patrick John Stover   +2 more
doaj   +1 more source

Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein

Pteridines, 1999
In plant cells, the interconversion of formyl- and methylene-tetrahydrofolates is catalyzed by a bifunctional protein possessing methenyltetrallydrofolate cydohydrolase (EC 3.5.4.9) and methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities. The
Chen Liangfu, Nargang Frank E., Cossin Edwin A.   +2 more
doaj   +1 more source

The Dissociation Constants of Tetrahydrofolic Acid

Journal of Biological Chemistry, 1966
Six dissociable groups exist in tetrahydrofolic acid in the range H0 -3.0 to pH 14. The assignments in tetrahydrofolic acid by titrimetric and spectrophotometric comparisons with model compounds, p-aminobenzoylglutamic acid and 2-amino-6,7-dimethyl-5,6,7,8-tetrahydro-4-pteridinone, are as follows: acidic ionization of amide, pK' 10.5; N5, pK' 4.82; γ ...
R G, Kallen, W P, Jencks
openaire   +2 more sources

In Vivo Rate of Formaldehyde Condensation with Tetrahydrofolate [PDF]

Metabolites, 2020
Formaldehyde is a highly reactive compound that participates in multiple spontaneous reactions, but these are mostly deleterious and damage cellular components. In contrast, the spontaneous condensation of formaldehyde with tetrahydrofolate (THF) has been proposed to contribute to the assimilation of this intermediate during growth on C1 carbon sources
He, Hai, Noor, Elad, Ramos-Parra, Perla A., García-Valencia, Liliana E., Patterson, Jenelle A., Díaz de la Garza, Rocío I., Hanson, Andrew D., Bar-Even, Arren   +7 more
openaire   +8 more sources

Tetrahydrofolate Recognition by the Mitochondrial Folate Transporter [PDF]

Journal of Biological Chemistry, 2011
A mitochondrial carrier family (MCF) of transport proteins facilitates the transfer of charged small molecules across the inner mitochondrial membrane. The human genome has ∼50 genes corresponding to members of this family. All MCF proteins contain three repeats of a characteristic and conserved PX(D/E)XX(K/R) motif thought to be central to the ...
Scott A, Lawrence, John C, Hackett, Richard G, Moran   +2 more
openaire   +2 more sources

Impact of the simultaneous administration of the (+)- and (−)-forms of formyl-tetrahydrofolic acid on plasma and intracellular pharmacokinetics of (−)-tetrahydrofolic acid

Cancer Chemotherapy and Pharmacology, 2000
To detect possible interactions between (-)-formyl-tetrahydrofolic acid (leucovorin, (-)-fTHF) and (+)-formyl-tetrahydrofolic acid ((+)-fTHF) on the plasma and intracellular pharmacokinetics following their simultaneous administration.Plasma levels of (-)-fTHF, (-)-methyl-THF, and (+)-fTHF were determined in samples from four volunteers following the ...
Schleyer, E., Rudolph, K. Lenhard, Braess, J., Unterhalt, Michael, Ehninger, G., Hiddemann, Wolfgang, Kern, W.   +6 more
openaire   +3 more sources

The Mechanism of the Condensation of Formaldehyde with Tetrahydrofolic Acid

Journal of Biological Chemistry, 1966
Abstract The bell-shaped pH rate profile for the reaction of tetrahydrofolic acid with formaldehyde to form 5,10-methylene tetrahydrofolic acid has been shown to result from a change in rate-determining step with changing acidity. In alkaline solution, the rate-determining step is the acid-catalyzed dehydration of hydroxymethyl tetrahydrofolic acid and
R G, Kallen, W P, Jencks
openaire   +2 more sources