An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum
Advanced Science, EarlyView.This work presents a strategy for engineering thermophilic Ago proteins to obtain wide catalytic temperature range and broad substrate spectrum. This strategy is applied to engineer PfAgo and TtdAgo, and the biochemical properties of the engineered PfAgo are systematically characterized. The study further elucidates the structural differences involved.
Longyu Wang +11 more
wiley +1 more source
Journal of Lipid Research, 2012 Fusion of modified LDL in the arterial wall promotes atherogenesis. Earlier we showed that thermal denaturation mimics LDL remodeling and fusion, and revealed kinetic origin of LDL stability.
Mengxiao Lu +3 more
doaj
High-throughput thermal denaturation of tryptophanyl-tRNA synthetase combinatorial mutants reveals high-order energetic coupling determinants of conformational stability. [PDF]
Struct Dyn, 2023 Weinreb V, Weinreb G, Carter CW.
europepmc +1 more source
Nucleic Acids Research, 1985 The nature of binding of Ru(phen) 2+ (I), Ru(bipy) 2+ (II), Ru(terpy) 2+ (III) (phen = 1,10-phenanthroline, bipy 3 = 2,2'-bipyridyl, 3 terpy = 2,2'2," - 2 terpyridyl) to DNA, poly[d(G-C)] and poly[d(A-T)] has been compared by absorption, fluorescence ...
J. Kelly +3 more
semanticscholar +1 more source
An Engineered Soluble Single‐Chain TCR Engager for KRAS‐G12V Specific Tumor Immunotherapy
Advanced Science, EarlyView.This study develops a dimeric T cell receptor engager protein specific for tumor KRAS‐G12V mutation. The engager proteins are capable of mediating the formation of synapse between T cell and tumor cell and demonstrate substantial tumor suppression efficacy.
Keke Ma +14 more
wiley +1 more source
Stability and assembly
Journal of Nanobiotechnology, 2007 Background The stability of a virus-like particle (VLP) is an important consideration for its use in nanobiotechnology. The icosahedral capsid of the RNA bacteriophage PP7 is cross-linked by disulfide bonds between coat protein dimers at its 5-fold and ...
Peabody David S, Caldeira Jerri C
doaj +1 more source
Instant Writing of Conductive Interface on MOF Single Crystal by Ultrafast Laser
Advanced Science, EarlyView.Current device fabrication strategies lack capacity for tailoring ideal electrical interface in individual MOF single crystals. An ultrafast laser approach is developed that in situ constructs stable conductive interface on MOF single crystal scale while maintaining its crystalline integrity.
Dongsheng Huang +11 more
wiley +1 more source
The role of water in the reversibility of thermal denaturation of lysozyme in solid and liquid states. [PDF]
Biochem Biophys Rep, 2021 Phan-Xuan T +6 more
europepmc +1 more source
USE OF THERMAL DENATURATION STUDIES TO INVESTIGATE THE BASE SEQUENCE OF YEAST SERINE sRNA [PDF]
, 1964 Gary Felsenfeld, Giulio L. Cantoni
openalex +1 more source
Thermal denaturation of human haemoglobins [PDF]
Biochemical Journal, 1970 Keith F. Tipton +2 more
openaire +3 more sources