Results 21 to 30 of about 105,310 (260)
Determination of Pseudokinase-ligand Interaction by a Fluorescence-based Thermal Shift Assay
Bio-Protocol, 2014 This protocol describes a robust technique for the measurement of pseudokinase-ligand interaction by a fluorescence-based Thermal Shift Assay (TSA).
Isabelle Lucet +7 more
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Journal of Pharmaceutical Analysis, 2018 Protein denaturation is under intensive research, since it leads to neurological disorders of severe consequences. Avoiding denaturation and stabilizing the proteins in their native state is of great importance, especially when proteins are used as drug ...
Karpagaraj Malarkani +2 more
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Food proteins: Solubility & thermal stability improvement techniques
Food Chemistry Advances, 2022 Proteins, as the major structural component, play important roles in food systems because of possessing bioactive nature, and valuable nutritional and functional characteristics.
Negin Yousefi, Soleiman Abbasi
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Hydrogen exchange in thermally denatured ribonuclease [PDF]
Biochemistry, 1991 Hydrogen exchange has been used to test for the presence of nonrandom structure in thermally denatured ribonuclease A (RNase A). Quenched-flow methods and 2D 1H NMR spectroscopy were used to measure exchange rates for 36 backbone amide protons (NHs) at 65 degrees C and at pH* (uncorrected pH measured in D2O) values ranging from 1.5 to 3.8.
A D, Robertson, R L, Baldwin
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A phenomenological model for predicting melting temperatures of DNA sequences. [PDF]
PLoS ONE, 2010 We report here a novel method for predicting melting temperatures of DNA sequences based on a molecular-level hypothesis on the phenomena underlying the thermal denaturation of DNA.
Garima Khandelwal, Jayaram Bhyravabhotla
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Research Progress on the Improvement and Evaluation of the Heat Stability of Whey Protein [PDF]
Shipin KexueWhey protein (WP), an important protein in dairy products, is rich in amino acids and bioactive peptides, and possesses high nutritional value. However, its poor thermal stability leads to issues such as protein denaturation, precipitation, and oxidation
DONG Xuan, PANG Xiaoyang, LU Ruqing, WANG Yunna, YU Jinghua, LÜ Jiaping, ZHANG Shuwen, LI Hongjuan
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Foods, 2022 The protective effects of phosphorylated trehalose on the quality and characteristics of peeled shrimp (Litopenaeus vannamei) were determined. Quality changes in treated samples were evaluated by assessing the physicochemical properties of myofibrillar ...
Shanshan Shui +3 more
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Honey-Induced Protein Stabilization as Studied by Fluorescein Isothiocyanate Fluorescence
The Scientific World Journal, 2013 Protein stabilizing potential of honey was studied on a model protein, bovine serum albumin (BSA), using extrinsic fluorescence of fluorescein isothiocyanate (FITC) as the probe.
Yin How Wong +2 more
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Thermal denaturation of nucleosomal core particles
Nucleic Acids Research, 1978 Thermal denaturation of very homogeneous preparations of core particles from chicken erythrocyte chromatin is studied by several techniques. The change in absorbance, which is very closely paralleled by changes in heat capacity, which is very closely paralleled by changes in heat capacity, is a biphasic process with inflexions at 60 degrees C and 74 ...
W O, Weischet +3 more
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Molecular Oncology, EarlyView.Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang +12 more
wiley +1 more source