Results 41 to 50 of about 2,421,340 (294)
Hydrogen exchange in thermally denatured ribonuclease [PDF]
Biochemistry, 1991 Hydrogen exchange has been used to test for the presence of nonrandom structure in thermally denatured ribonuclease A (RNase A). Quenched-flow methods and 2D 1H NMR spectroscopy were used to measure exchange rates for 36 backbone amide protons (NHs) at 65 degrees C and at pH* (uncorrected pH measured in D2O) values ranging from 1.5 to 3.8.
A D, Robertson, R L, Baldwin
openaire +2 more sources
Thermal denaturation of Stx isoforms and mutants.
, 2013 A. Stx1B (black) and Stx1B-L41Q (gray) thermal denaturation curves. Stx1B-L41Q is destabilized (decreased melting temperature) compared to wild-type Stx1B. B.
Rhett A. Kovall (339140) +6 more
core +1 more source
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Thermal Denaturation Profiles of Tuna Myoglobin
Bioscience, Biotechnology, and Biochemistry, 2010 Myoglobin (Mb) purified from fast skeletal muscle of bluefin tuna Thunnus thynnus orientalis was subjected to thermal treatment, and the denaturation profiles were examined by thermodynamic analysis. Based on the ellipticity or helical content obtained by circular dichroism (CD) spectrometry, it was found that denaturation of tuna Mb consisted of three
OCHIAI, Yoshihiro +5 more
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Decoupling enzyme catalysis from thermal denaturation [PDF]
, 2010 The equilibrium model (EM) (Daniel et al., 2001) postulates two forms of a folded enzyme, one catalytically active (Eact) and the other inactive (Einact), which interconvert via a fast thermal equilibrium (Keq) (Figure A). This model for enzyme catalysis
Easter, Ashley Davys
core
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Thermal denaturation of individual and tandem ACP.
, 2013 Thermal denaturation of (A) the tandem ACP and (B) the individual ACP1 was followed by the loss of molar ellipiticity at 222 nm on a circular dichroism (CD) spectropolarimeter.
Abel Baerga-Ortiz (384228) +7 more
core +1 more source
The regulatory subunit of PKA-I remains partially structured and undergoes β-aggregation upon thermal denaturation. [PDF]
PLoS ONE, 2011 BackgroundThe regulatory subunit (R) of cAMP-dependent protein kinase (PKA) is a modular flexible protein that responds with large conformational changes to the binding of the effector cAMP.
Khanh K Dao +7 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source