Results 41 to 50 of about 1,963,376 (294)
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
Thermal denaturation of ribonuclease T1. A DSC study
, 1992 The thermal denaturation of microbial Ribonuclease T1 (RNAase T1) as a function of pH, was studied by means of DSC microcalorimetry. The midpoint denaturation temperatures, enthalpy changes and heat capacity changes of Ribonuclease T1 were compared with ...
GIANCOLA, CONCETTA +12 more
core +2 more sources
Thermal Denaturation Profiles of Tuna Myoglobin
Bioscience, Biotechnology, and Biochemistry, 2010 Myoglobin (Mb) purified from fast skeletal muscle of bluefin tuna Thunnus thynnus orientalis was subjected to thermal treatment, and the denaturation profiles were examined by thermodynamic analysis. Based on the ellipticity or helical content obtained by circular dichroism (CD) spectrometry, it was found that denaturation of tuna Mb consisted of three
OCHIAI, Yoshihiro +5 more
openaire +2 more sources
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
New thermal insulation fiberboards from cake generated during biorefinery of sunflower whole plant in a twin-screw extruder [PDF]
, 2014 The objective of this study was to manufacture new thermal insulation fiberboards by thermo-pressing. The starting material was a slightly deoiled cake (17.6% oil content), generated during the biorefinery of sunflower (Helianthus annuus L.) whole plant ...
Evon, Philippe +3 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Journal of Lipid Research, 2012 Fusion of modified LDL in the arterial wall promotes atherogenesis. Earlier we showed that thermal denaturation mimics LDL remodeling and fusion, and revealed kinetic origin of LDL stability.
Mengxiao Lu +3 more
doaj +1 more source
Feasibility of microfluidic routes to monitor protein stability as a tool for bioprocessing [PDF]
, 2010 During the bioprocessing of therapeutics, proteins may become damaged leading to modifications and changes in stability. This may, as a consequence, cause serious and potentially fatal side effects when administered to patients making damage ...
Remtulla, N.
core
Monitoring protein denaturation using thermal conductivity probe
, 2018 We propose a method for probing denaturation of proteins by measuring the thermal conductivity of the solution. We use the three-omega method with a microfabricated ac thermal sensor to measure the thermal conductivity of lysozyme, beta-lactoglobulin ...
Byoung kyoo Park +3 more
core +1 more source
Whole-body cryostimulation impact on blood serum thermal denaturation profiles of cross-country skiers [PDF]
, 2019 Blood plasma and serum are important diagnostic materials as they clearly reflect an individual’s metabolism. The study has evaluated the effect of whole-body cryostimulation (WBC) on the blood serum of professional cross-country skiers. The experiment
Michnik, Anna +4 more
core +1 more source