Results 21 to 30 of about 196,670 (297)
Determination of Pseudokinase-ligand Interaction by a Fluorescence-based Thermal Shift Assay
This protocol describes a robust technique for the measurement of pseudokinase-ligand interaction by a fluorescence-based Thermal Shift Assay (TSA).
Isabelle Lucet +7 more
doaj +1 more source
The current review focused on the effect of different thermal and non-thermal processing techniques on the structural and functional modifications of milk proteins. In thermal processing, denaturation in whey proteins takes place at the temperature range
Qamar Abbas Syed +7 more
doaj +1 more source
Protein denaturation is under intensive research, since it leads to neurological disorders of severe consequences. Avoiding denaturation and stabilizing the proteins in their native state is of great importance, especially when proteins are used as drug ...
Karpagaraj Malarkani +2 more
doaj +1 more source
Statistical Mechanics of thermal denaturation of DNA oligomers [PDF]
Double stranded DNA chain is known to have nontrivial elasticity. We study the effect of this elasticity on the denaturation profile of DNA oligomer by constraining one base pair at one end of the oligomer to remain in unstretched (or intact) state.
Singh, Navin, Singh, Yashwant
core +2 more sources
Correlated parameter fit of arrhenius model for thermal denaturation of proteins and cells. [PDF]
Qin Z +4 more
europepmc +2 more sources
Direct observation of large temperature fluctuations during DNA thermal denaturation [PDF]
In this paper we report direct measurement of large low frequency temperature fluctuations in double stranded (ds) DNA when it undergoes thermal denaturation transition.
Chatterji, Dipankar +2 more
core +2 more sources
Food proteins: Solubility & thermal stability improvement techniques
Proteins, as the major structural component, play important roles in food systems because of possessing bioactive nature, and valuable nutritional and functional characteristics.
Negin Yousefi, Soleiman Abbasi
doaj +1 more source
Hydrogen exchange in thermally denatured ribonuclease [PDF]
Hydrogen exchange has been used to test for the presence of nonrandom structure in thermally denatured ribonuclease A (RNase A). Quenched-flow methods and 2D 1H NMR spectroscopy were used to measure exchange rates for 36 backbone amide protons (NHs) at 65 degrees C and at pH* (uncorrected pH measured in D2O) values ranging from 1.5 to 3.8.
A D, Robertson, R L, Baldwin
openaire +2 more sources
Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella [PDF]
Aiming to combine the flexibility of Brucella lumazine synthase (BLS) to adapt different protein domains in a decameric structure and the capacity of BLS and flagellin to enhance the immunogenicity of peptides that are linked to their structure, we ...
Berguer, Paula Mercedes +11 more
core +3 more sources
Analysis of the molecular mobility of collagen and elastin in safe, atheromatous and aneurysmal aortas [PDF]
Aim of the study : In this study, we propose to use a thermal technique, Differential Scanning Calorimetry (DSC) to follow the evolution of elastin and collagen in safe and pathological cardiovascular tissues.
Dandurand, Jany +7 more
core +2 more sources

