Results 51 to 60 of about 2,421,340 (294)

Thermal stability denaturation experiments.

open access: yes, 2012
A. Kinetics of retinal release by thermal denaturation from suspensions of 10 µM wt-bOp and T90A-bOp reconstituted with AT and 13E-RET in 50 mM phosphate buffer.
Marta Domínguez (145644)   +7 more
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

open access: yesFEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li   +7 more
wiley   +1 more source

Research Progress on the Improvement and Evaluation of the Heat Stability of Whey Protein [PDF]

open access: yesShipin Kexue
Whey protein (WP), an important protein in dairy products, is rich in amino acids and bioactive peptides, and possesses high nutritional value. However, its poor thermal stability leads to issues such as protein denaturation, precipitation, and oxidation
DONG Xuan, PANG Xiaoyang, LU Ruqing, WANG Yunna, YU Jinghua, LÜ Jiaping, ZHANG Shuwen, LI Hongjuan
doaj   +1 more source

The effect of Na + and K + on the thermal denaturation of Na + and + K + -dependent ATPase [PDF]

open access: yes, 1983
To increase our understanding of the physical nature of the Na+ and K+ forms of the Na+ + K+-dependent ATPase, thermal-denaturation studies were conducted in different types of ionic media.
Fischer, T H
core   +1 more source

Rationally engineered polymeric interfaces for selective and dynamic recognition of vesicular agents

open access: yesFEBS Open Bio, EarlyView.
This review explores how molecularly imprinted polymers can be engineered to recognize complex nanoscale vesicles, including extracellular vesicles and membrane‐enveloped viruses. By tailoring polymer cavities to vesicle surface chemistry and structure, these synthetic receptors support selective detection, separation, and integration with optical ...
Özgecan Erdem   +4 more
wiley   +1 more source

Nanoamphizymes: Self‐Assembled Amphiphilic Enzymatic Nanomaterials for High‐Performance Biocatalysis and Biosensing

open access: yesAdvanced Functional Materials, EarlyView.
An amphiphilic GOx nanoamphizyme was constructed by covalently introducing hydrophobic PPNI linkers onto GOx, enabling spontaneous self‐assembly into tunable nanoparticles. Mild crosslinking further stabilized the assemblies and enhanced catalytic performance and stability.
Yuan‐Hao Hsiao   +4 more
wiley   +1 more source

Food proteins: Solubility & thermal stability improvement techniques

open access: yesFood Chemistry Advances, 2022
Proteins, as the major structural component, play important roles in food systems because of possessing bioactive nature, and valuable nutritional and functional characteristics.
Negin Yousefi, Soleiman Abbasi
doaj   +1 more source

Mean transition midpoints of thermal denaturation (CD spectroscopy, FAD fluorescence, ANS fluorescence).

open access: yes, 2014
Mean transition midpoints of thermal denaturation (CD spectroscopy, FAD fluorescence, ANS fluorescence).
Johanna M. Jank (549307)   +10 more
core   +1 more source

Critical Coupling in Semi‐Trasparent Fano Resonators for Photothermal Converters

open access: yesAdvanced Functional Materials, EarlyView.
An ultrathin metal–insulator multilayer architecture is engineered to exploit Fano‐resonant critical coupling, completely suppressing optical reflectance while achieving a balanced 50:50 trade‐off between absorbance and transmittance. Upon laser excitation at resonance, the metamaterial generates intense localized heat (ΔT≈550∘C$\Delta T {\approx }550^\
Giuseppe Emanuele Lio   +3 more
wiley   +1 more source

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