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Tissue and Cell, 2021
In this study, a three-dimensional (3D) thermo-reversible gelation polymer (TGP) culture system was established for organoid culture of mouse fallopian tube (FT) epithelial stem cells (FTESCs) without cell isolation. FT tissues from 6- to 8-week-old ICR mice were digested with collagenase, and whole FT cells (FTCs) were inoculated into the TGP. After 6
YunXiu, Lin +5 more
openaire +2 more sources
In this study, a three-dimensional (3D) thermo-reversible gelation polymer (TGP) culture system was established for organoid culture of mouse fallopian tube (FT) epithelial stem cells (FTESCs) without cell isolation. FT tissues from 6- to 8-week-old ICR mice were digested with collagenase, and whole FT cells (FTCs) were inoculated into the TGP. After 6
YunXiu, Lin +5 more
openaire +2 more sources
Artificial Organs, 2003
Abstract: The purpose of this study is to utilize the thermo‐reversible gelation polymer in which the sol‐gel transitting phase is reversibly changed by temperature in a three‐dimensional culture system. Human cancer cells have been observed to form multicellular spheroids, whereas fibroblasts slowly develop into small spheroids with the culture medium
Satoshi, Tsukikawa +11 more
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Abstract: The purpose of this study is to utilize the thermo‐reversible gelation polymer in which the sol‐gel transitting phase is reversibly changed by temperature in a three‐dimensional culture system. Human cancer cells have been observed to form multicellular spheroids, whereas fibroblasts slowly develop into small spheroids with the culture medium
Satoshi, Tsukikawa +11 more
openaire +2 more sources
Sensors and Actuators B: Chemical, 2010
Abstract This paper presents a new strategy for sorting fluorescently labeled cells with a microfluidic device. Cell separation was performed with flow switching using changes in fluidic resistance in a waste channel caused by sol–gel transition of a thermo-reversible gelation polymer (TGP).
Kazuto Ozaki +6 more
openaire +1 more source
Abstract This paper presents a new strategy for sorting fluorescently labeled cells with a microfluidic device. Cell separation was performed with flow switching using changes in fluidic resistance in a waste channel caused by sol–gel transition of a thermo-reversible gelation polymer (TGP).
Kazuto Ozaki +6 more
openaire +1 more source
Tissue Engineering Part C: Methods, 2009
In the present study, we examined the possible utility of a three-dimensional culture system using a thermo-reversible gelation polymer to isolate and expand neural stem cells (NSCs). The polymer is a synthetic biologically inert polymer and gelates at temperatures higher than the gel-sol transition point ( approximately 20 degrees C). When fetal mouse
Yang, Xin-Zhi +6 more
openaire +3 more sources
In the present study, we examined the possible utility of a three-dimensional culture system using a thermo-reversible gelation polymer to isolate and expand neural stem cells (NSCs). The polymer is a synthetic biologically inert polymer and gelates at temperatures higher than the gel-sol transition point ( approximately 20 degrees C). When fetal mouse
Yang, Xin-Zhi +6 more
openaire +3 more sources
Human Cell, 2008
TGP (thermo-reversible gelation polymer) is a high molecular compound that has so-gel transmitting temperature of 221C Since solid cancer tissue grows in this polymer three-dimensionally, and fibroblasts scarcely grow in it, TGP is suitable for chemosensitivity assays for solid tumors.
Tatsuru, Ohara +6 more
openaire +2 more sources
TGP (thermo-reversible gelation polymer) is a high molecular compound that has so-gel transmitting temperature of 221C Since solid cancer tissue grows in this polymer three-dimensionally, and fibroblasts scarcely grow in it, TGP is suitable for chemosensitivity assays for solid tumors.
Tatsuru, Ohara +6 more
openaire +2 more sources
Journal of stem cells, 2014
Regeneration of bony defects caused by periodontal disease continues to be a challenge for clinicians. Application of stem cells from different tissue sources and scaffolds for regeneration have been reported in animal models but clinical studies with long term follow-ups are limited.
Seshadri, Sankaranarayanan +4 more
openaire +1 more source
Regeneration of bony defects caused by periodontal disease continues to be a challenge for clinicians. Application of stem cells from different tissue sources and scaffolds for regeneration have been reported in animal models but clinical studies with long term follow-ups are limited.
Seshadri, Sankaranarayanan +4 more
openaire +1 more source
Tailoring inorganic–polymer composites for the mass production of solid-state batteries
Nature Reviews Materials, 2021Li-Zhen Fan, Hongcai He, Ce-Wen Nan
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Reducing environmental plastic pollution by designing polymer materials for managed end-of-life
Nature Reviews Materials, 2021Kara Lavender, Ramani Narayan
exaly