Results 131 to 140 of about 5,570 (173)
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The effect of pH on thermolysin activity
Archives of Biochemistry and Biophysics, 1971Abstract The activity of the neutral protease thermolysin toward a synthetic substrate N-furlylacryloylglycyl- l -leucinamide has been measured as a function of pH and temperature. The quantity k 2 K m has been found to depend on two groups: one with pK 5.25 and heat of ionization of +8.0 kcal/mole, and a second group with pK 7.98 ...
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Peptide Bond Synthesis Catalyzed by Thermolysin
The Journal of Biochemistry, 1980The coupling between Cbz-Phe-OH and Leu-NH2 catalyzed by thermolysin was examined under various experimental conditions. The highest yield (ca. 80%) was obtained in the reaction mixture containing 0.05 M each of the carboxyl and amine components and 10 muM enzyme at pH 7 and 37 degrees C for 5 h. The reactivity was ca.
T, Oka, K, Morihara
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Studies on thermolysin. I. Effects of chemical modifications on the activity of thermolysin.
Journal of biochemistry, 19771. When thermolysin was treated with a 100-fold molar excess of 2,4,6-trinitrobenzene-1-sulfonate at pH 8.0 and 37 degrees for 7 h, all 12 amino groups in the enzyme were almost completely trinitrophenylated. The fully trinitrophenylated enzyme still retained more than 80% of its original activity.
H, Oshima, T, Abe, K, Takahashi
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Inhibition stereochemistry of hydroxamate inhibitors for thermolysin
Bioorganic & Medicinal Chemistry Letters, 1998N-Acyl-N-hydroxy-beta-amino acid derivatives were prepared and tested as inhibitors for thermolysin to find that these inhibitors show the L-stereospecificity in contrast to the corresponding hydroxamates prepared from alpha-amino acid, which exhibit the D-stereochemistry.
Y, Jin, D H, Kim
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Stability of immobilized thermolysin in organic solvents
Journal of Bioscience and Bioengineering, 1999Various factors affecting the stability of thermolysin immobilized by cross-linking with glutaraldehyde were elucidated, particularly in the water-immiscible organic solvents such as ethyl acetate and tert-amyl alcohol. The main reason for enzyme inactivation in water-immiscible organic solvents was found to be autolysis in the water phase, which may ...
M, Miyanaga +4 more
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Inhibition of Thermolysin by Dipeptides
Biochemistry, 1974J, Feder, L R, Brougham, B S, Wildi
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Inhibition of thermolysin and carboxypeptidase A by phosphoramidates
Biochemistry, 1979C M, Kam, N, Nishino, J C, Powers
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