Results 71 to 80 of about 3,152 (182)

Diversity and structural‐functional insights of alpha‐solenoid proteins

Protein Science, Volume 33, Issue 11, November 2024.
Abstract Alpha‐solenoids are a significant and diverse subset of structured tandem repeat proteins (STRPs) that are important in various domains of life. This review examines their structural and functional diversity and highlights their role in critical cellular processes such as signaling, apoptosis, and transcriptional regulation.
Paula Nazarena Arrías, Zarifa Osmanli, Estefanía Peralta, Patricio Manuel Chinestrad, Alexander Miguel Monzon, Silvio C. E. Tosatto   +5 more
wiley   +1 more source

Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum

, 2022
S.1475-1480The glyceraldehyde dehydrogenase from Thermoplasma acidophilum (TaAlDH) is a microbial enzyme that catalyzes the oxidation of D-glyceraldehyde to D-glycerate in the artificial enzyme cascade designed for the conversion of glucose to the ...
Degtjarik, O., Iermak, I., Kuta Smatanova, I., Steffler, Fabian, Sieber, Volker   +4 more
core   +1 more source

Metal ion activation and DNA recognition by the Deinococcus radiodurans manganese sensor DR2539

The FEBS Journal, Volume 291, Issue 15, Page 3384-3402, August 2024.
The manganese sensor DR2539 from Deinococus radiodurans recognises the dr1709 promoter region, which consists of a 21 base pair pseudo‐palindrome binding two DR2539 monomers. Here, we identified four manganese binding sites and investigated protein‐DNA interactions, with critical nucleotides being probed.
Cristiano Mota, Myles Webster, Melissa Saidi, Ulrike Kapp, Chloe Zubieta, Gabriele Giachin, José Antonio Manso, Daniele de Sanctis   +7 more
wiley   +1 more source

Development of a Highly Selective NAD+‐Dependent Glyceraldehyde Dehydrogenase and its Application in Minimal Cell‐Free Enzyme Cascades

ChemSusChem, Volume 17, Issue 4, February 22, 2024.
This work demonstrates the power of enzyme engineering to tailor a biocatalyst to meet various requirements of a cell‐free artificial multi‐enzyme cascade. We targeted glyceraldehyde dehydrogenase (GADH) as a key enzyme to efficiently produce ethanol from D‐glucose (obtainable from lignocellulosic agricultural wastes).
Mariko Teshima, Samuel Sutiono, Manuel Döring, Barbara Beer, Mikael Boden, Gerhard Schenk, Volker Sieber   +6 more
wiley   +1 more source

Lipoprotein-like particles in a prokaryote: quinone droplets of Thermoplasma acidophilum

, 2016
Cytosolic, globular droplets with an average diameter of 50 nm were observed in vitrified Thermoplasma acidophilum cells by means of cryo-electron tomography.
Orsini, M., Moracci, Marco, Varga, Sándor, Nagy, I., Boicu, M., Baumeister, W., Orsini, Massimiliano, Kukolya, J., Kofler, C., Weyher-Stingl, Elisabeth, Sun, N., Fernández Busnadiego, Rubén, Weyher-Stingl, E., Varga, S., Kofler, Christine, Nagy, István, Kukolya, József, Fernandez-Busnadiego, R., Nickell, S., Knispel, R., Sun, Na, Boicu, Marius, Knispel, Roland Wilhelm   +22 more
core   +1 more source

THERMOPLASMA VOLCANİUM'UN 20S PROTEOZOM DÜZENLEYİCİ PROTEİN GENLERİNİN STRES KOŞULLARINDA ANLATIM PROFİLLERİNDEKİ DEĞİŞİM

, 2013
THERMOPLASMA VOLCANİUM'UN 20S PROTEOZOM DÜZENLEYİCİ PROTEİN GENLERİNİN STRES KOŞULLARINDA ANLATIM PROFİLLERİNDEKİ ...
Yilmaz, Tülay, Kocabıyık, Semra
core  

THERMOPLASMA VOLCANİUM'UN Hps20 BENZERİ BİR KÜÇÜK ISI ŞOKU PROTEİNİNE İLİŞKİN YAPISAL ANALİZLER

, 2013
THERMOPLASMA VOLCANİUM'UN Hps20 BENZERİ BİR KÜÇÜK ISI ŞOKU PROTEİNİNE İLİŞKİN YAPISAL ...
Kocabıyık, Semra, Sheraj, Ilir
core  

The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum

, 2013
A transformation method yielding up to 10(4) transformants per mu g circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.
Kriszt, B., Ivanics, M., Varga, S., Fekete, C., Hobel, C., Nagy, I., Baka, E., Kukolya, J., Knispel, R.   +8 more
core   +1 more source

Localization of a sequence motif complementary to the nuclear localization signal in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy

, 1992
A sequence motif complementary to the nuclear localization signal (NLS) has been localized in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy using sequence-specific antibodies.
Cejka, Z., Grziwa, A., Dahlmann, B., Santarius, U., Baumeister, W.   +4 more
core   +1 more source

The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum

, 1996
In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels and have investigated the effects of this mutation on ...
Erduran, I, Kocabiyik, S, Russel, RJM, Danson, MJ, Kocabıyık, Semra, Hough, DW   +5 more
core   +1 more source