Results 51 to 60 of about 3,152 (182)

The import pathway of human and Thermoplasma 20S proteasomes into HeLa cell nuclei is different from that of classical NLS-bearing proteins

, 1999
Wild-type proteasomes of human erythrocytes and the archaeon Thermoplasma acidophilum compete with each other for transport into nuclei of digitonin-permeabilized HeLa cells in the presence of an energy-regenerating system and rabbit reticulocyte lysate.
Nederlof, P., Mayr, J., Wang, H. R., Baumeister, W., Wang, H.   +4 more
core   +1 more source

Proteomics Analysis of Thermoplasma acidophilum with a Focus on Protein Complexes [PDF]

Molecular & Cellular Proteomics, 2007
Two-dimensional gel electrophoresis (2DE) and MALDI-TOF MS were used to obtain a global view of the cytoplasmic proteins expressed by Thermoplasma acidophilum. In addition, glycerol gradient ultracentrifugation coupled to 2DE-MALDI-TOF MS analysis was used to identify subunits of macromolecular complexes.
Na, Sun, Florian, Beck, Roland Wilhelm, Knispel, Frank, Siedler, Beatrix, Scheffer, Stephan, Nickell, Wolfgang, Baumeister, István, Nagy   +7 more
openaire   +2 more sources

Identification, characterization and classification of prokaryotic nucleoid‐associated proteins

Molecular Microbiology, Volume 123, Issue 3, Page 206-217, March 2025.
Nucleoid‐associated proteins (NAPs) structure DNA by either bending, wrapping, bridging, or forming protein filaments on the DNA. We review newly identified NAPs and provide a list of simple biochemical assays to study the DNA‐structuring properties of novel NAPs. Abstract Common throughout life is the need to compact and organize the genome.
Samuel Schwab, Remus T. Dame
wiley   +1 more source

Quinone Profiles of Thermoplasma acidophilum HO-62 [PDF]

Journal of Bacteriology, 2001
ABSTRACT Quinones of Thermoplasma acidophilum HO-62 were analyzed by high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance. Menaquinone, methionaquinone, and 2- trans and 2- cis forms of thermoplasmaquinone were identified.
H, Shimada, Y, Shida, N, Nemoto, T, Oshima, A, Yamagishi   +4 more
openaire   +2 more sources

Chromatin and gene regulation in archaea

Molecular Microbiology, Volume 123, Issue 3, Page 218-231, March 2025.
Recent data from diverse archaea with different repertoires of nucleoid‐associated proteins show a common genome‐wide chromatin organisation where chromatin maintains access to gene promoters independently of transcription activity. Abstract The chromatinisation of DNA by nucleoid‐associated proteins (NAPs) in archaea ‘formats’ the genome structure in ...
Fabian Blombach, Finn Werner
wiley   +1 more source

The Intein of the Thermoplasma A-ATPase A Subunit: Structure, Evolution and Expression in E. Coli

, 2001
Inteins are selfish genetic elements that excise themselves from the host protein during post translational processing, and religate the host protein with a peptide bond.
Alireza G Senejani, Senejani, Ali, Gogarten, J. Peter, Hilario, Elena, J Peter Gogarten, Elena Hilario   +5 more
core   +1 more source

The origins of phagocytosis and eukaryogenesis

Biology Direct, 2009
Background Phagocytosis, that is, engulfment of large particles by eukaryotic cells, is found in diverse organisms and is often thought to be central to the very origin of the eukaryotic cell, in particular, for the acquisition of bacterial endosymbionts
Wolf Yuri I, Wolf Maxim Y, Yutin Natalya, Koonin Eugene V   +3 more
doaj   +1 more source

Genetic Transfer in Action: Uncovering DNA Flow in an Extremophilic Microbial Community

Environmental Microbiology, Volume 27, Issue 2, February 2025.
Different taxa from all domains of life sharing the same environment are connected by DNA transfers that have occurred between them. Whereas most studies appraising horizontal genetic transfer (HGT) events employ methods that only identify protein‐coding genes, here we apply a gene‐agnostic method to metagenomic data for the first time.
Julia Van Etten, Timothy G. Stephens, Debashish Bhattacharya   +2 more
wiley   +1 more source

Geomicrobiology of a seawater-influenced active sulfuric acid cave.

PLoS ONE, 2019
Fetida Cave is an active sulfuric acid cave influenced by seawater, showing abundant microbial communities that organize themselves under three main different morphologies: water filaments, vermiculations and moonmilk deposits.
Ilenia M D'Angeli, Daniele Ghezzi, Stefan Leuko, Andrea Firrincieli, Mario Parise, Adriano Fiorucci, Bartolomeo Vigna, Rosangela Addesso, Daniela Baldantoni, Cristina Carbone, Ana Zelia Miller, Valme Jurado, Cesareo Saiz-Jimenez, Jo De Waele, Martina Cappelletti   +14 more
doaj   +1 more source

Architectures of archaeal GINS complexes, essential DNA replication initiation factors

BMC Biology, 2011
Background In the early stage of eukaryotic DNA replication, the template DNA is unwound by the MCM helicase, which is activated by forming a complex with the Cdc45 and GINS proteins.
Saito Mihoko, Nagasawa Naoko, Mayanagi Kouta, Shirai Tsuyoshi, Ogino Hiromi, Fujino Seiji, Ishino Sonoko, Oyama Takuji, Ishino Yoshizumi, Morikawa Kosuke   +9 more
doaj   +1 more source