Results 31 to 40 of about 3,408 (192)
A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase™ DNA polymerase is a thermostable enzyme engineered to catalyze the incorporation of ddNTPs with an efficiency several thousandfold ...
P.B. Vander Horn +12 more
doaj +1 more source
The elucidation of mechanisms behind the thermostability of proteins is extremely important both from the theoretical and applied perspective. Here we report the crystal structure of methylenetetrahydrofolate dehydrogenase (MTHFD) from Thermus ...
Fernando Maiello +5 more
doaj +1 more source
Impact of age-associated cyclopurine lesions on DNA repair helicases. [PDF]
8,5' cyclopurine deoxynucleosides (cPu) are locally distorting DNA base lesions corrected by nucleotide excision repair (NER) and proposed to play a role in neurodegeneration prevalent in genetically defined Xeroderma pigmentosum (XP) patients.
Irfan Khan +7 more
doaj +1 more source
The DNA-binding protein HTa from Thermoplasma acidophilum is an archaeal histone analog
Histones are a principal constituent of chromatin in eukaryotes and fundamental to our understanding of eukaryotic gene regulation. In archaea, histones are widespread but not universal: several lineages have lost histone genes.
Antoine Hocher +4 more
doaj +1 more source
Ubiquitin found in the archaebacterium Thermoplasma acidophilum
Systematic N‐terminal sequencing of the low molecular weight proteins from Thermoplasma acidophilum separated by two‐dimensional polyacrylamide get electrophoresis led to the discovery of a polypeptide with an apparent M r of 4.5 kDa identical as its first 18 amino acid residues to human ubiquitin.
Wolf, S., Lottspeich, F., Baumeister, W.
openaire +3 more sources
Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
The coffee industry produces over 10 billion kg beans per year and generates high amounts of different waste products. Spent coffee grounds (SCG) are an industrially underutilized waste resource, which is rich in the polysaccharide galactomannan, a ...
Dominik Kopp +3 more
doaj +1 more source
The thermosome of the archaeon Thermoplasma acidophilum is composed of two subunits, alpha and beta, which are arranged in two stacked, eight-membered rings.
Nitsch, M. +5 more
core +1 more source
Biosulfidogenesis Mediates Natural Attenuation in Acidic Mine Pit Lakes
Acidic pit lakes are abandoned open pit mines filled with acid mine drainage (AMD)—highly acidic, metalliferous waters that pose a severe threat to the environment and are rarely properly remediated.
Charlotte M. van der Graaf +8 more
doaj +1 more source
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami +6 more
wiley +1 more source
Structure of thermoplasmaquinone from Thermoplasma acidophilum [PDF]
The structure of the methyl-substituted menaquinone (designated thermoplasmaquinone) from the extremely thermophilic and acidophilic archaebacterium Thermoplasma acidophilum was investigated by mass spectrometry and proton nuclear magnetic resonance spectrometry. The results of the present study show that the novel quinone from T.
openaire +1 more source

