Results 31 to 40 of about 3,152 (182)
BioTechniques, 1997 A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase™ DNA polymerase is a thermostable enzyme engineered to catalyze the incorporation of ddNTPs with an efficiency several thousandfold ...
P.B. Vander Horn +12 more
doaj +1 more source
PLoS ONE, 2020 The elucidation of mechanisms behind the thermostability of proteins is extremely important both from the theoretical and applied perspective. Here we report the crystal structure of methylenetetrahydrofolate dehydrogenase (MTHFD) from Thermus ...
Fernando Maiello +5 more
doaj +1 more source
Impact of age-associated cyclopurine lesions on DNA repair helicases. [PDF]
PLoS ONE, 2014 8,5' cyclopurine deoxynucleosides (cPu) are locally distorting DNA base lesions corrected by nucleotide excision repair (NER) and proposed to play a role in neurodegeneration prevalent in genetically defined Xeroderma pigmentosum (XP) patients.
Irfan Khan +7 more
doaj +1 more source
The DNA-binding protein HTa from Thermoplasma acidophilum is an archaeal histone analog
eLife, 2019 Histones are a principal constituent of chromatin in eukaryotes and fundamental to our understanding of eukaryotic gene regulation. In archaea, histones are widespread but not universal: several lineages have lost histone genes.
Antoine Hocher +4 more
doaj +1 more source
, 2021 Ma’ruf IF, Sasaki Y, Kerbs A, et al. Heterologous gene expression and characterization of two serine hydroxymethyltransferases from Thermoplasma acidophilum. Extremophiles.
Ma’ruf, Ilma Fauziah +13 more
core +1 more source
Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
Frontiers in Bioengineering and Biotechnology, 2019 The coffee industry produces over 10 billion kg beans per year and generates high amounts of different waste products. Spent coffee grounds (SCG) are an industrially underutilized waste resource, which is rich in the polysaccharide galactomannan, a ...
Dominik Kopp +3 more
doaj +1 more source
Biosulfidogenesis Mediates Natural Attenuation in Acidic Mine Pit Lakes
Microorganisms, 2020 Acidic pit lakes are abandoned open pit mines filled with acid mine drainage (AMD)—highly acidic, metalliferous waters that pose a severe threat to the environment and are rarely properly remediated.
Charlotte M. van der Graaf +8 more
doaj +1 more source
Current Protocols, Volume 6, Issue 6, June 2026.Abstract Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite involved in numerous biological processes, functioning both as a redox cofactor in cellular energy metabolism and as a co‐substrate for NAD+‐utilizing enzymes, including sirtuins and poly(ADP‐ribose) polymerases.
Emily Boyle, Olivia Zhang, Yana Cen
wiley +1 more source
Structure of thermoplasmaquinone from Thermoplasma acidophilum [PDF]
FEMS Microbiology Letters, 1985 The structure of the methyl-substituted menaquinone (designated thermoplasmaquinone) from the extremely thermophilic and acidophilic archaebacterium Thermoplasma acidophilum was investigated by mass spectrometry and proton nuclear magnetic resonance spectrometry. The results of the present study show that the novel quinone from T.
openaire +1 more source
The FEBS Journal, Volume 293, Issue 11, Page 3303-3319, June 2026.Characterization of anhydromevalonate phosphate decarboxylase, the UbiD‐family decarboxylase involved in the archaeal mevalonate pathway, was conducted. The enzyme is responsible for the biosynthesis of isoprenoids, such as archaeal membrane lipids, respiratory quinones, and dolichols.
Rino Ishikawa +9 more
wiley +1 more source