Results 1 to 10 of about 68,203 (335)

Combined Computer-Aided Predictors to Improve the Thermostability of Nattokinase [PDF]

Foods, 2023
Food-derived nattokinase has strong thrombolytic activity and few side effects. In the field of medicine, nattokinase has been developed as an adjuvant drug for the treatment of thrombosis, and nattokinase-rich beverages and health foods have also shown ...
Yuan Li, Liangqi Chen, Xiyu Tang, Wenhui Zhu, Aixia Ma, Changyu Shi, Jinyao Li   +6 more
doaj   +2 more sources

A Structural Landscape Depiction of Dynamic Stability Centers of Local Structure in Protein Thermostability Engineering [PDF]

Research
Engineering protein thermostability is a key aspect of rational protein design, aiming to broaden the applicability of enzymes and enhance their industrial utility.
Xu Qiu, Huan Liu, Peizhi Song, Xiaoran Cheng, Wanjing Wu, Siyang He, Weiwei Wang, Ping Xu, Hongzhi Tang   +8 more
doaj   +2 more sources

Sequence-Structure Based Comparison of Structurally Homologous Thermophilic and Mesophilic Polyethylene Terephthalate (PET) Hydrolases

Hayati Journal of Biosciences, 2023
Protein structure has a direct impact on thermostability. Deviations in the primary sequence can affect structural changes, leading to alterations in thermostability properties.
Khomaini Hasan, Maria Ulfah, Niknik Nurhayati, Gabriela Christy Sabbathini, Sri Rezeki Wulandari, I Gede Eka Perdana Putra, Is Helianti   +6 more
doaj   +1 more source

Rational Design of Pepsin for Enhanced Thermostability via Exploiting the Guide of Structural Weakness on Stability

Frontiers in Physics, 2021
Enzyme thermostability is an important parameter for estimating its industrial value. However, most naturally produced enzymes are incapable of meeting the industrial thermostability requirements.
Yue Zhao, Yulu Miao, Fengdong Zhi, Yue Pan, Jianguo Zhang, Xuepeng Yang, John Z. H. Zhang, John Z. H. Zhang, John Z. H. Zhang, Lujia Zhang, Lujia Zhang   +10 more
doaj   +1 more source

Application of Rigidity Theory to the Thermostabilization of Lipase A from Bacillus subtilis. [PDF]

PLoS Computational Biology, 2016
Protein thermostability is a crucial factor for biotechnological enzyme applications. Protein engineering studies aimed at improving thermostability have successfully applied both directed evolution and rational design.
Prakash Chandra Rathi, Alexander Fulton, Karl-Erich Jaeger, Holger Gohlke   +3 more
doaj   +1 more source

ProtDataTherm: A database for thermostability analysis and engineering of proteins. [PDF]

PLoS ONE, 2018
Protein thermostability engineering is a powerful tool to improve resistance of proteins against high temperatures and thereafter broaden their applications.
Hassan Pezeshgi Modarres, Mohammad R Mofrad, Amir Sanati-Nezhad   +2 more
doaj   +1 more source

Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase

Computational and Structural Biotechnology Journal, 2021
Cellulases are industrially important enzymes, e.g., in the production of bioethanol, in pulp and paper industry, feedstock, and textile. Thermostability is often a prerequisite for high process stability and improving thermostability without affecting ...
Francisca Contreras, Christina Nutschel, Laura Beust, Mehdi D. Davari, Holger Gohlke, Ulrich Schwaneberg   +5 more
doaj   +1 more source

Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis

AMB Express, 2023
As an indispensable enzyme for the hydrolysis of dextran, dextranase has been widely used in the fields of food and medicine. It should be noted that the weak thermostability of dextranase has become a restricted factor for industrial applications.
Zhen Wei, Jinling Chen, Linxiang Xu, Nannan Liu, Jie Yang, Shujun Wang   +5 more
doaj   +1 more source

Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis

Microbial Cell Factories, 2020
Background Enzymatic quantification of creatinine has become an essential method for clinical evaluation of renal function. Although creatinase (CR) is frequently used for this purpose, its poor thermostability severely limits industrial applications ...
Xue Bai, Daixi Li, Fuqiang Ma, Xi Deng, Manjie Luo, Yan Feng, Guangyu Yang   +6 more
doaj   +1 more source

Enhancing the thermostability of Streptomyces cyaneofuscatus strain Ms1 tyrosinase by multi-factors rational design and molecular dynamics simulations.

PLoS ONE, 2023
This study presents a multi-factor rational design strategy combined with molecular dynamics simulation to improve the thermostability of Streptomyces cyaneofuscatus strain Ms1 tyrosinase.
Zhengtao Li, Chen Zhao, Duanhua Li, Lu Wang   +3 more
doaj   +1 more source