Results 91 to 100 of about 104,127 (354)

Stabilization versus flexibility: Detergent-dependent trade-offs in neurotensin receptor 1 GPCR ensembles. [PDF]

Protein Sci
Abstract Detergents provide essential membrane‐mimetic environments for studying G protein‐coupled receptors (GPCRs), but their molecular impact on receptor energetics remains incompletely understood. We combined ligand binding, thermostability measurements, and atomistic molecular dynamics to dissect detergent‐ versus ligand‐driven stabilization in a ...
Bower JB, van der Velden WJC, Gomez KP, Pan M, Bumbak F, Vaidehi N, Ziarek JJ.   +6 more
europepmc   +2 more sources

Peptide Sequence Programmed Piezoelectric Response by Supramolecular Self‐Assembly

Advanced Science, EarlyView.
In this study, hydrophobic tripeptides are explored as building blocks for stable piezoelectric crystals. Tripeptides with phenylalanine at the central position self‐assembled into uniform, thermally stable crystals. Piezoresponse force microscopy reveals a high effective piezoelectric coefficient of 24.0 pC N−1, attributed to asymmetric molecular ...
Xuejiao Yang, Shuaijie Liu, Honglei Lu, Yuehui Wang, Hongyue Zhang, Wei Ji, Huaimin Wang   +6 more
wiley   +1 more source

Ligands regulate GroEL thermostability

FEBS Letters, 1997
Escherichia coli heat‐shock proteins GroEL and GroES stimulate (in an ATP‐dependent manner) the folding of various proteins. In this study scanning microcalorimetry was applied to investigate GroEL thermostability in the presence of its ligands. Mg2+ and K+ ions stabilize while ADP destabilizes the GroEL molecule against the action of temperature ...
Surin, A.K, Kotova, N.V, Kashparov, I.A, Marchenkov, V.V, Marchenkova, S.Yu, Semisotnov, G.V   +5 more
openaire   +2 more sources

The Regulatory Role of Iron Transporter SLC39A13 in Liver Fibrosis

Advanced Science, EarlyView.
SLC39A13/ZIP13, a newly discovered intracellular iron transporter, delivers iron to the ER/Golgi to catalyze procollagen hydroxylation during collagen maturation. Here, we systematically characterize the cell type‐specific functions of ZIP13 across distinct hepatic cell populations, and identify hepatic stellate cell‐specific ZIP13 as a promising and ...
Shanshan Guo, Yalin Wang, Binyu Lu, Yu Zhang, David M. Frazer, Bing Zhou   +5 more
wiley   +1 more source

Evolution of Prime Editing: Enhancing Efficiency and Expanding Capacity

Advanced Science, EarlyView.
Most rare diseases are caused by genetic mutations. Prime editing (PE) has emerged as a versatile tool capable of inducing diverse mutations without generating DNA double‐strand breaks. Despite its significant clinical potential, PE faces limitations in terms of efficiency and scalability.
Jihyeon Yu, Ju‐Chan Park, Heesoo Uhm, Yong‐Woo Kim, Hyeon Woo Im, Sangsu Bae   +5 more
wiley   +1 more source

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

Advanced Science, EarlyView.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han, Boyuan Xue, Guangqi Shan, Meng Meng, Shaojie Wang, Haijia Su   +5 more
wiley   +1 more source

Expanding the Chemical Space of Cyclic Polyphthalaldehyde via Post‐Functionalization

Advanced Science, EarlyView.
In this work, we developed a post‐functionalization approach to achieve water‐soluble cyclic polyphthalaldehyde (cPPA) polymers via click chemistry. ABSTRACT Cyclic polyphthalaldehydes (cPPAs) are hydrophobic cyclic polymers with stimuli‐responsive depolymerization property. The introduction of hydrophilic functionalities to cPPAs is challenging due to
Liting He, Haiyan Zhou, Qiushi Chen, Xing Guo, Han Liu, Xuechen Li   +5 more
wiley   +1 more source

Structure Based Thermostability Prediction Models for Protein Single Point Mutations with Machine Learning Tools.

PLoS ONE, 2015
Thermostability issue of protein point mutations is a common occurrence in protein engineering. An application which predicts the thermostability of mutants can be helpful for guiding decision making process in protein design via mutagenesis.
Lei Jia, Ramya Yarlagadda, Charles C Reed   +2 more
doaj   +1 more source

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties

Frontiers in Bioengineering and Biotechnology, 2017
Lipases are important industrial enzymes. Most of the lipases operate at lipid–water interfaces enabled by a mobile lid domain located over the active site. Lid protects the active site and hence responsible for catalytic activity. In pure aqueous media,
Faez Iqbal Khan, Faez Iqbal Khan, Dongming Lan, Rabia Durrani, Weiqian Huan, Zexin Zhao, Yonghua Wang   +6 more
doaj   +1 more source

Rigidity and flexibility of biological networks

, 2012
The network approach became a widely used tool to understand the behaviour of complex systems in the last decade. We start from a short description of structural rigidity theory.
Almaas, Andrusier, Atilgan, Barabasi, Bateson, Bergman, Bhalla and Ravi Iyengar, Csermely, Csermely, Das, David, de Jong, Eyal, Felli, Fulle, Gonzalez, Gromiha, Gustafsson, Ingber, Jacobs, Jimenez-Roldan, Kannan, Karlebach, Kellermayer, Koshland, Krishnan, Kundu, Kuriyan, Laskowski, Leherte, Lei, Linding, Livesay, Luo, Mason, Mihalik, Monsell, Najmanovich, Pandurangan, Papin, Piazza, Piazza, Potestio, Price, Rader, Radestock, Ragone, Rose, Smith, Stephanopoulos, Sun, Szil  gyi, Thomas, Tsai, Wagner, Wang, Watts, Wells, Wells, Whitty, Yang, Yuan, Zaccai, Zhu, Zhuravlev   +64 more
core   +1 more source