Results 11 to 20 of about 104,127 (354)

Improvement of thermostability and catalytic efficiency of glucoamylase from Talaromyces leycettanus JCM12802 via site-directed mutagenesis to enhance industrial saccharification applications

Biotechnology for Biofuels, 2021
Background Glucoamylase is an important industrial enzyme in the saccharification of starch into glucose. However, its poor thermostability and low catalytic efficiency limit its industrial saccharification applications.
Lige Tong, Jie Zheng, Xiao Wang, Xiaolu Wang, Huoqing Huang, Haomeng Yang, Tao Tu, Yuan Wang, Yingguo Bai, Bin Yao, Huiying Luo, Xing Qin   +11 more
doaj   +2 more sources

Combined Computer-Aided Predictors to Improve the Thermostability of Nattokinase [PDF]

Foods, 2023
Food-derived nattokinase has strong thrombolytic activity and few side effects. In the field of medicine, nattokinase has been developed as an adjuvant drug for the treatment of thrombosis, and nattokinase-rich beverages and health foods have also shown ...
Yuan Li, Liangqi Chen, Xiyu Tang, Wenhui Zhu, Aixia Ma, Changyu Shi, Jinyao Li   +6 more
doaj   +2 more sources

A Structural Landscape Depiction of Dynamic Stability Centers of Local Structure in Protein Thermostability Engineering [PDF]

Research
Engineering protein thermostability is a key aspect of rational protein design, aiming to broaden the applicability of enzymes and enhance their industrial utility.
Xu Qiu, Huan Liu, Peizhi Song, Xiaoran Cheng, Wanjing Wu, Siyang He, Weiwei Wang, Ping Xu, Hongzhi Tang   +8 more
doaj   +2 more sources

ProtDataTherm: A database for thermostability analysis and engineering of proteins. [PDF]

PLoS ONE, 2018
Protein thermostability engineering is a powerful tool to improve resistance of proteins against high temperatures and thereafter broaden their applications.
Hassan Pezeshgi Modarres, Mohammad R Mofrad, Amir Sanati-Nezhad   +2 more
doaj   +2 more sources

Directed Evolution of Pseudomonas fluorescens Lipase Variants With Improved Thermostability Using Error-Prone PCR

Frontiers in Bioengineering and Biotechnology, 2020
Lipases catalyze the hydrolysis of fats and oils, and have been widely used in various industrial fields. However, bacterial lipases have a lower thermostability in industrial processes, which was a limiting factor in their industrial application.
Lijun Guan, Yang Gao, Jialei Li, Kunlun Wang, Zhihong Zhang, Song Yan, Nina Ji, Ye Zhou, Shuwen Lu   +8 more
doaj   +2 more sources

Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120

BMC Biotechnology, 2018
Background Lipoxygenase (LOX) from Anabaena sp. PCC 7120 (Ana-rLOX) offers important applications in the food industry, especially for improving aroma and dough rheological properties.
Hui Qian, Chong Zhang, Zhaoxin Lu, Bingjie Xia, Xiaomei Bie, Haizhen Zhao, Fengxia Lu, Guang-Yu Yang   +7 more
doaj   +2 more sources

Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region

Microbial Cell Factories, 2018
Background High thermostability is required for trypsin to have wider industrial applications. Target mutagenesis at flexible regions has been proved to be an efficient protein engineering method to enhance the protein thermostability.
Lin Liu, Haoran Yu, Kun Du, Zhiyan Wang, Yiru Gan, He Huang   +5 more
doaj   +2 more sources

Sequence-Structure Based Comparison of Structurally Homologous Thermophilic and Mesophilic Polyethylene Terephthalate (PET) Hydrolases

Hayati Journal of Biosciences, 2023
Protein structure has a direct impact on thermostability. Deviations in the primary sequence can affect structural changes, leading to alterations in thermostability properties.
Khomaini Hasan, Maria Ulfah, Niknik Nurhayati, Gabriela Christy Sabbathini, Sri Rezeki Wulandari, I Gede Eka Perdana Putra, Is Helianti   +6 more
doaj   +1 more source

Rational Design of Pepsin for Enhanced Thermostability via Exploiting the Guide of Structural Weakness on Stability

Frontiers in Physics, 2021
Enzyme thermostability is an important parameter for estimating its industrial value. However, most naturally produced enzymes are incapable of meeting the industrial thermostability requirements.
Yue Zhao, Yulu Miao, Fengdong Zhi, Yue Pan, Jianguo Zhang, Xuepeng Yang, John Z. H. Zhang, John Z. H. Zhang, John Z. H. Zhang, Lujia Zhang, Lujia Zhang   +10 more
doaj   +1 more source

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Communications Biology, 2023
The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry. In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes ...
Binyang Deng, Y. Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, C. Zhai   +9 more
semanticscholar   +1 more source