Results 91 to 100 of about 40,909 (278)

Insertion of the FeB cofactor in cNORs lacking metal inserting chaperones

FEBS Letters, Volume 599, Issue 9, Page 1269-1284, May 2025.
Nitric oxide reductase is an enzyme found in the bacterial denitrification pathway. The NOR active site contains a non‐heme iron, often, but not always inserted with the assistance of chaperones. Here, we study the insertion of FeB in the subfamily of cNORs lacking chaperones and found a putative channel, conserved in the family, perhaps enabling the ...
Sofia Appelgren, Pia Ädelroth
wiley   +1 more source

Thermostable in vitro transcription-translation compatible with microfluidic droplets

Microbial Cell Factories
Background In vitro expression involves the utilization of the cellular transcription and translation machinery in an acellular context to produce one or more proteins of interest and has found widespread application in synthetic biology and in ...
Ana L. J. L. Ribeiro, Patricia Pérez-Arnaiz, Mercedes Sánchez-Costa, Lara Pérez, Marcos Almendros, Liisa van Vliet, Fabrice Gielen, Jesmine Lim, Simon Charnock, Florian Hollfelder, J. Eduardo González-Pastor, José Berenguer, Aurelio Hidalgo   +12 more
doaj   +1 more source

Sugar‐sensing swodkoreceptors and swodkocrine signaling

Animal Models and Experimental Medicine, Volume 8, Issue 5, Page 944-961, May 2025.
Sugar‐sensing swodkoreceptors that trigger swodkocrine signaling. Abstract Sugars are one of the major metabolites and are essential for nucleic acid synthesis and energy production. In addition, sugars can act as signaling molecules. To study sugar signaling at the systemic level, there is an urgent need to systematically identify sugar‐sensing ...
Savani Anbalagan
wiley   +1 more source

Role of Archaeal HerA Protein in the Biology of the Bacterium Thermus thermophilus

Genes, 2017
Intense gene flux between prokaryotes result in high percentage of archaeal genes in the genome of the thermophilic bacteria Thermus spp. Among these archaeal genes a homolog to the Sulfolobus spp. HerA protein appears in all of the Thermus spp.
A. Blesa, N. Quintáns, Ignacio Baquedano, C. Mata, J. R. Castón, J. Berenguer   +5 more
semanticscholar   +1 more source

Thermophilic phosphoribosyltransferases Thermus thermophilus HB27 in nucleotide synthesis

Beilstein Journal of Organic Chemistry, 2018
Phosphoribosyltransferases are the tools that allow the synthesis of nucleotide analogues using multi-enzymatic cascades. The recombinant adenine phosphoribosyltransferase (TthAPRT) and hypoxanthine phosphoribosyltransferase (TthHPRT) from Thermus ...
Ilja V. Fateev, Ekaterina V. Sinitsina, Aiguzel U. Bikanasova, Maria A. Kostromina, Elena S. Tuzova, Larisa V. Esipova, Tatiana I. Muravyova, Alexei L. Kayushin, Irina D. Konstantinova, Roman S. Esipov   +9 more
doaj   +1 more source

Characterization of a novel PrimPol from Thermus thermophilus

, 2023
Accurate DNA amplification has become essential for most molecular diagnostic and therapeutic techniques. With the recent approvals of gene therapy products, this fast-growing field is facing an increased demand for large-scale production and manufacturing of clinical grade synthetic DNA. TruePrime¿, commercialized by 4BaseBio S.L.
Martínez-Carrón, Ana, Picher, Ángel J., Blanco Dávila, Luis   +2 more
openaire   +3 more sources

Safety evaluation of the food enzyme 4‐α‐glucanotransferase from the genetically modified Bacillus amyloliquefaciens strain MAS

EFSA Journal, Volume 23, Issue 5, May 2025.
Abstract The food enzyme 4‐α‐glucanotransferase (1,4‐α‐d‐glucan:1,4‐α‐d‐glucan 4‐α‐d‐glycosyltransferase; EC2.4.1.25) is produced with the genetically modified Bacillus amyloliquefaciens strain MAS by Coöperatie Koninklijke Avebe U.A. The production strain contains multiple copies of known antimicrobial resistance genes.
EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Monika Sramkova, Henk Van Loveren, Laurence Vernis, Simone Lunardi, Magdalena Andryszkiewicz, Giulio Di Piazza, Natalia Kovalkovicova, Yi Liu   +18 more
wiley   +1 more source

Isolation, genome sequencing, assembly, annotation and Characterization of Thermus sp. CCB_US3_UF1 FROM Ulu Slim, Perak, Malaysia. [PDF]

, 2011
Thermus sp. CCB_US3_UF1, satu bacteria thermofilik telah Berjaya dipencilkan dari kolam air panas di Ulu Slim, Perak, Malaysia. Genomnya mengandungi 2,243,772 pasangan bes dan satu plasmid bersaiz 19,716 pasangan bes. Thermus sp.
Teh, Beng Soon
core  

Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp

Journal of Bacteriology, 1986
Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain.
Y Koyama, N Tomizuka, Kensuke Furukawa, T Hoshino   +3 more
openaire   +3 more sources

Analysis of Protein Thermostability Enhancing Factors in Industrially Important Bacteria Species

Evolutionary Bioinformatics, 2013
Elucidation of evolutionary factors that enhance protein thermostability is a critical problem and was the focus of this work on Thermus species. Pairs of orthologous sequences of T. scotoductus SA-01 and T.
Benjamin Kumwenda, Derek Litthauer, Özlem Tastan Bishop, Oleg Reva   +3 more
doaj   +1 more source