Results 101 to 110 of about 26,854 (254)

Structural insights into RNA processing by the human RISC-loading complex. [PDF]

, 2009
Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC).
AD Haase, B Kastner, Bunpote Siridechadilok, C Matranga, Cameron Noland, David W Taylor, DS Schwarz, E Bernstein, E Ma, EF Pettersen, Enbo Ma, Eva Nogales, F Herzog, FV Rivas, H Gao, Hong-Wei Wang, IJ MacRae, IJ MacRae, IJ MacRae, JB Preall, Jennifer A Doudna, JJ Song, Karin Felderer, KH Kok, M Högbom, M Radermacher, M van Heel, PA Penczek, SH Scheres, SJ Ludtke, T Sasaki, TA Rand, TM Rana, TP Chendrimada, W Wriggers, Y Lee, Y Tomari, Y Tomari, Y Wang, Y Wang, YR Yuan, YR Yuan   +41 more
core   +2 more sources

One‐Pot Isothermal Nucleic Acid Amplification Assisted CRISPR/Cas Detection Technology: Challenges, Strategies, and Perspectives

Advanced Science, Volume 12, Issue 37, October 6, 2025.
This review combines the current status of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat)‐based molecular diagnostic technology to expound the necessity and challenges faced in promoting the development of one‐pot detection. Besides, the focus is on a comprehensive summary and incisive analysis of emerging strategies for one‐pot ...
Hongyan Liu, Hui Yin, Leshan Xiu, Wenyu Wu, Qinqin Hu, Yingchen Xia, Brianna Garcia, Shamsuttiyeba Shifa, Hui Chen, Min Li, Kun Yin   +10 more
wiley   +1 more source

Cryo-EM studies of the rotary H+-ATPase/synthase from Thermus thermophilus

Biophysics and Physicobiology, 2019
Proton-translocating rotary ATPases couple proton influx across the membrane domain and ATP hydrolysis/synthesis in the soluble domain through rotation of the central rotor axis against the surrounding peripheral stator apparatus.
Atsuko Nakanishi, Jun-ichi Kishikawa, Kaoru Mitsuoka, Ken Yokoyama   +3 more
doaj   +1 more source

Microbial communities and arsenic biogeochemistry at the outflow of an alkaline sulfide-rich hot spring. [PDF]

, 2016
Alkaline sulfide-rich hot springs provide a unique environment for microbial community and arsenic (As) biogeochemistry. In this study, a representative alkaline sulfide-rich hot spring, Zimeiquan in the Tengchong geothermal area, was chosen to study ...
Dai, Xinyue, Jiang, Dawei, Jiang, Zhou, Li, Ping, Van Nostrand, Joy D, Wang, Yanhong, Wang, Yanxin, Zhang, Ping, Zhang, Rui, Zhou, Jizhong   +9 more
core   +1 more source

Response to Nutrient Stress in the Industrial Model Bacterium Cupriavidus necator: A Thermal Proteome Profiling (TPP) Investigation

PROTEOMICS, Volume 25, Issue 20, Page 25-38, October 2025.
ABSTRACT The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources, or of autotrophic growth using CO2 fixation with H2 as an energy source. Under stress conditions, it produces biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total
Kate McKeever, Jia‐Lynn Tham, Manuel Bruch, Tania Narancic, Kevin O’ Connor, Swathi Ramachandra Upadhya, Colm Ryan, Eugene T. Dillon, Kieran Wynne, Gerard Cagney   +9 more
wiley   +1 more source

Caldomycin, a new guanidopolyamine produced by a novel agmatine homocoupling enzyme involved in homospermidine biosynthesis

Scientific Reports
An extreme thermophilic bacterium, Thermus thermophilus produces more than 20 unusual polyamines, but their biosynthetic pathways, including homospermidine, are not yet fully understood.
Teruyuki Kobayashi, Akihiko Sakamoto, Tamao Hisano, Keiko Kashiwagi, Kazuei Igarashi, Koichi Takao, Takeshi Uemura, Takemitsu Furuchi, Yoshiaki Sugita, Toshiyuki Moriya, Tairo Oshima, Yusuke Terui   +11 more
doaj   +1 more source

Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins [PDF]

, 2008
In the genome of a thermophilic bacterium, Thermus thermophilus HB27, three genes, TTC0418, TTC0746 and TTC1975, were annotated as ATP-dependent protease La (Lon). Sequence comparisons indicated that TTC0418 and TTC0746 showed significant similarities to
Hoshino Takayuki, Maehara Tomoko, Nakamura Akira, 中村 顕   +3 more
core   +1 more source

Type III CRISPR complexes from Thermus thermophilus.

Acta Biochimica Polonica, 2016
Pathogen-specific acquired immunity in bacteria is mediated by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems. Thermus thermophilus strain HB8 contains CRISPR systems of several major subtypes (type I, IIIA and IIIB), and has become a widely studied model for CRISPR biology.
Szychowska, Marta, Siwek, Wojciech, Pawolski, Damian, Kazrani, Asgar Abbas, Pyrć, Krzysztof, Bochtler, Matthias   +5 more
openaire   +4 more sources

Tracking Copper sensing operon Repressor (CsoR) oligomerization in solution using Electron Paramagnetic Resonance spectroscopy

Protein Science, Volume 34, Issue 10, October 2025.
Abstract Protein oligomerization is a fundamental biological process that plays a pivotal role in regulating numerous cellular functions. In this study, we investigated the oligomeric state of copper‐sensing operon repressor (CsoR), a transcriptional repressor bound to the cso operon in Mycobacterium tuberculosis.
Yasmin Igbaria‐Jaber, Alysia Mandato, Lukas Hofmann, Yulia Shenberger, Lada Gevorkyan‐Airapetov, Liel Sapir, Sunil Saxena, Sharon Ruthstein   +7 more
wiley   +1 more source

Random Chromosome Partitioning in the Polyploid Bacterium Thermus thermophilus HB27

G3: Genes, Genomes, Genetics, 2019
Little is known about chromosome segregation in polyploid prokaryotes. In this study, whether stringent or variable chromosome segregation occurs in polyploid thermophilic bacterium Thermus thermophilus was analyzed.
Haijuan Li
doaj   +1 more source