Results 11 to 20 of about 40,909 (278)

Development of a new gene expression vector for Thermus thermophilus using a silica-inducible promoter

Microbial Cell Factories, 2020
Background Thermostable enzymes are commonly produced in mesophilic hosts for research and bioengineering purposes. However, these hosts do not overexpress the active forms of some biologically functional thermoenzymes.
Yasuhiro Fujino, Shuichiro Goda, Yuri Suematsu, Katsumi Doi   +3 more
doaj   +2 more sources

Superoxide Dismutase and Pseudocatalase Increase Tolerance to Hg(II) in Thermus thermophilus HB27 by Maintaining the Reduced Bacillithiol Pool

mBio, 2019
Mercury (Hg) is a widely distributed, toxic heavy metal with no known cellular role. Mercury toxicity has been linked to the production of reactive oxygen species (ROS), but Hg does not directly perform redox chemistry with oxygen.
Javiera Norambuena, Thomas E. Hanson, Tamar Barkay, Jeffrey M. Boyd   +3 more
doaj   +2 more sources

Cryo-EM structure of the hibernating Thermus thermophilus 100S ribosome reveals a protein-mediated dimerization mechanism

Nature Communications, 2018
During stress conditions bacterial ribosomes dimerize and form inactive but stable hibernating 100S particles, a process that is facilitated by the hibernation-promoting factor (HPF).
Rasmus Kock Flygaard, Niels Boegholm, Marat Yusupov, Lasse B. Jenner   +3 more
doaj   +2 more sources

Sequence of the hyperplastic genome of the naturally competent Thermus scotoductus SA-01 [PDF]

BMC Genomics, 2011
Background Many strains of Thermus have been isolated from hot environments around the world. Thermus scotoductus SA-01 was isolated from fissure water collected 3.2 km below surface in a South African gold mine.
A Friedrich, A Friedrich, A Friedrich, A Henne, ACE Darling, Antje Wollherr, B Averhoff, B Averhoff, B Ewing, B Ewing, Benjamin Kumwenda, C Bricio, C Cervantes, C Moller, C Nesbo, C Schwarzenlander, C Schwarzenlander, Carlos Bricio, D Chivian, D Mooser, D Slade, Derek Litthauer, DJ Opperman, DJ Opperman, DJ Opperman, DL Balkwill, DP Moser, E van Heerden, Elzbieta Brzuszkiewicz, Esta van Heerden, F Cava, F Cava, G Wanger, Gerhard Gottschalk, GI Omar, H Brüggemann, H Ganesan, Heiko Liesegang, I Narumi, J Janzon, J Mrazek, J Mrazek, JC Fisher, JG Lawrence, JK Bonfield, JK Fredrickson, José Berenguer, JR Lloyd, K Zahradka, Kamini Gounder, KM Handley, KM Handley, L Alvarez, LH Lin, LS Busenlehner, M De Grado, M de Grado, M de la Bastide, M Tanaka, M Wolfgang, M Wolfgang, Malay Srivastava, MGI Langille, MGI Langille, MJ Marshall, MV Omelchenko, N Ohtani, N Saitou, O Bezuidt, Oleg Reva, PA Bester, PD Karp, PD Karp, PD Karp, R Barrangou, RAD Williams, Rolf Daniel, S Graupner, S Karlin, S Silver, S Waack, S Whelan, SF Altschul, SF Altschul, TC Onstott, TL Kieft, TM Gihring, TM Gihring, W Hsiao, Y Agari, Y Koyama, YI Wolf   +91 more
core   +7 more sources

Regulatory Factors for tRNA Modifications in Extreme- Thermophilic Bacterium Thermus thermophilus

Frontiers in Genetics, 2019
Thermus thermophilus is an extreme-thermophilic bacterium that can grow at a wide range of temperatures (50–83°C). To enable T. thermophilus to grow at high temperatures, several biomolecules including tRNA and tRNA modification enzymes show extreme heat-
Hiroyuki Hori
doaj   +2 more sources

Thermus thermophilus Strains Active in Purine Nucleoside Synthesis [PDF]

Molecules, 2009
Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation of the products. N-2-deoxy-ribosyltransferase activity
Almendros, Marcos, Sinisterra, José Vicente, Berenguer, José   +2 more
openaire   +5 more sources

Atypical Features of Thermus thermophilus Succinate:Quinone Reductase [PDF]

PLoS ONE, 2013
The Thermus thermophilus succinate:quinone reductase (SQR), serving as the respiratory complex II, has been homologously produced under the control of a constitutive promoter and subsequently purified. The detailed biochemical characterization of the resulting wild type (wt-rcII) and His-tagged (rcII-His(8)-SdhB and rcII-SdhB-His(6)) complex II ...
Frauke Baymann, Mohamed R. Noor, Sarah R. O'Kane, Tewfik Soulimane, Olga Kolaj-Robin   +4 more
openaire   +5 more sources

Cryo EM structure of intact rotary H+-ATPase/synthase from Thermus thermophilus

Nature Communications, 2018
H+-ATPases employ a rotary catalytic mechanism to couple ATP synthesis/hydrolysis with proton translocation through the membrane. Here, the authors use high-resolution cryoEM to characterize three rotational states of a bacterial H+-ATPase, providing a ...
Atsuko Nakanishi, Jun-ichi Kishikawa, Masatada Tamakoshi, Kaoru Mitsuoka, Ken Yokoyama   +4 more
doaj   +2 more sources

Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8

Frontiers in Microbiology, 2016
A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium Thermus thermophilus HB8 as its host.
Yuko eNagayoshi, Kenta eKumagae, Kazuki eMori, Kosuke eTashiro, Ayano eNakamura, Yasuhiro eFujino, Yasuaki eHiromasa, Takeo eIwamoto, Satoru eKuhara, Toshihisa eOhshima, Katsumi eDoi   +10 more
doaj   +2 more sources

Random Chromosome Partitioning in the Polyploid Bacterium Thermus thermophilus HB27

G3: Genes, Genomes, Genetics, 2019
Little is known about chromosome segregation in polyploid prokaryotes. In this study, whether stringent or variable chromosome segregation occurs in polyploid thermophilic bacterium Thermus thermophilus was analyzed.
Haijuan Li
doaj   +2 more sources