Results 21 to 30 of about 26,854 (254)

Thermus thermophilus CRISPR Cas6 Heterologous Expression and Purification [PDF]

Bio-Protocol
The CRISPR-Cas system of Thermus thermophilus has emerged as a potent biotechnological tool, particularly its Cas6 endonuclease, which plays a crucial role in CRISPR RNA (crRNA) maturation.
Junwei Wei, Mohamed Motawaa, Yingjun Li
doaj   +2 more sources

Atypical features of Thermus thermophilus succinate:quinone reductase. [PDF]

PLoS ONE, 2013
The Thermus thermophilus succinate:quinone reductase (SQR), serving as the respiratory complex II, has been homologously produced under the control of a constitutive promoter and subsequently purified.
Olga Kolaj-Robin, Mohamed R Noor, Sarah R O'Kane, Frauke Baymann, Tewfik Soulimane   +4 more
doaj   +4 more sources

Effects of Argonaute on Gene Expression in Thermus thermophilus. [PDF]

PLoS ONE, 2015
Eukaryotic Argonaute proteins mediate RNA-guided RNA interference, allowing both regulation of host gene expression and defense against invading mobile genetic elements.
Daan C Swarts, Jasper J Koehorst, Edze R Westra, Peter J Schaap, John van der Oost   +4 more
doaj   +6 more sources

Thermus thermophilus Strains Active in Purine Nucleoside Synthesis [PDF]

Molecules, 2009
Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases.
Marcos Almendros, José Berenguer Carlos, José-Vicente Sinisterra Gago   +2 more
doaj   +4 more sources

High‐efficiency genome editing of an extreme thermophile Thermus thermophilus using endogenous type I and type III CRISPR‐Cas systems [PDF]

mLife, 2022
Thermus thermophilus is an attractive species in the bioindustry due to its valuable natural products, abundant thermophilic enzymes, and promising fermentation capacities.
Jinting Wang, Junwei Wei, Haijuan Li, Yingjun Li   +3 more
doaj   +2 more sources

Profiling Thermus thermophilus Argonaute Guide DNA Sequence Preferences by Functional Screening [PDF]

Frontiers in Molecular Biosciences, 2021
Prokaryotic Argonautes (pAgo) are an increasingly well-studied class of guided endonucleases, and the underlying mechanisms by which pAgo generate nucleic acid guides in vivo remains an important topic of investigation.
Eric A. Hunt, Esta Tamanaha, Kevin Bonanno, Eric J. Cantor, Nathan A. Tanner   +4 more
doaj   +2 more sources

Thermus thermophilus genome analysis: benefits and implications [PDF]

Microbial Cell Factories, 2004
The genome sequence analysis of Thermus thermophilus HB27, a microorganism with high biotechnological potential, has recently been published. In that report, the chromosomal and the megaplasmid sequence were compared to those of other organisms and ...
Lioliou Efthimia E, Pantazaki Anastasia A, Kyriakidis Dimitrios A   +2 more
doaj   +3 more sources

Construction of primary chassis cells with efficient protein expression in Thermus thermophilus. [PDF]

Microb Cell Fact
Liang Y, Motawaa M, Bu X, Wei J, Shao Y, Li Y.   +5 more
europepmc   +3 more sources

Alkaline Stress Causes Changes in Polyamine Biosynthesis in Thermus thermophilus. [PDF]

Int J Mol Sci, 2022
Kobayashi T, Sakamoto A, Kashiwagi K, Igarashi K, Moriya T, Oshima T, Terui Y.   +6 more
europepmc   +3 more sources

Phosphomimetic mutations near active sites of proteins in Thermus thermophilus suggest a widespread regulatory mechanism. [PDF]

FEBS Open Bio
In Thermus thermophilus, more than half of the phosphorylation sites identified by proteomic analysis are located near the active site. All phosphomimetic mutants of phosphosites of six enzymes belonging to different families showed severely reduced activity compared with the wild‐type, particularly in the turnover number.
Nishiwaki A, Okanishi H, Kanai Y, Masui R.   +3 more
europepmc   +2 more sources