Characterization of a promiscuous cadmium and arsenic resistance mechanism in Thermus thermophilus HB27 and potential application of a novel bioreporter system [PDF]
Microbial Cell Factories, 2018 Background The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation.
Antonucci, Immacolata +8 more
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An ArsR/SmtB family member regulates arsenic resistance genes unusually arranged in Thermus thermophilus HB27. [PDF]
Microbial Biotechnology, 2017 Arsenic resistance is commonly clustered in ars operons in bacteria; main ars operon components encode an arsenate reductase, a membrane extrusion protein, and an As-sensitive transcription factor.
Antonucci, Immacolata +8 more
core +2 more sources
Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus [PDF]
Frontiers in Microbiology, 2015 Functional metagenomic screening strategies, which are independent of known sequence information, can lead to the identification of truly novel genes and enzymes. Since E.
Angel Angelov +10 more
core +4 more sources
Genome-scale metabolic network reconstruction and in silico flux analysis of the thermophilic bacterium Thermus thermophilus HB27 [PDF]
Microbial Cell Factories, 2014 10.1186/1475-2859-13-61Microbial Cell Factories131 ...
Aggarwal, S. +6 more
core +3 more sources
Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile [PDF]
Archives of Biological Sciences, 2012 A site-directed mutant R453T of a laccase from Thermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the effect on laccase catalytic properties. The mutated gene was cloned and overexpressed in Escherichia coli.
Liu Kewu, Liu Xin, Tian Meng
core +3 more sources
Expression and Purification of the Thermus thermophilus Argonaute Protein
BIO-PROTOCOL, 2014 The Argonaute protein of Thermus thermophilus (TtAgo) has recently been studied in detail. For its in vitro characterization, TtAgo was purified after heterologous expression in Escherichia coli (E. coli). As TtAgo expression is toxic, a tightly controlled system was used for protein expression. The expression strain E.
John van der Oost +2 more
openaire +4 more sources
A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus. [PDF]
Microb Cell Fact, 2023 Background Enzymes from thermophiles are of great interest for research and bioengineering due to their stability and efficiency. Thermophilic expression hosts such as Thermus thermophilus [T.
Kirchner L, Müller V, Averhoff B.
europepmc +2 more sources
Molecular characterization of the PhiKo endolysin from Thermus thermophilus HB27 bacteriophage phiKo and its cryptic lytic peptide RAP-29. [PDF]
Front Microbiol, 2023 IntroductionIn the era of increasing bacterial resistance to antibiotics, new bactericidal substances are sought, and lysins derived from extremophilic organisms have the undoubted advantage of being stable under harsh environmental conditions. The PhiKo
Szadkowska M +7 more
europepmc +2 more sources
Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant. [PDF]
Sci Rep, 2021 Thermophilic organisms are extensively studied in industrial biotechnology, for exploration of the limits of life, and in other contexts. Their optimal growth at high temperatures presents a challenge for the development of genetic tools for their genome
Adalsteinsson BT +7 more
europepmc +2 more sources
New Viruses Infecting Hyperthermophilic Bacterium <i>Thermus thermophilus</i>. [PDF]
VirusesHighly diverse phages infecting thermophilic bacteria of the Thermus genus have been isolated over the years from hot springs around the world. Many of these phages are unique, rely on highly unusual developmental strategies, and encode novel enzymes ...
Kolesnik M +9 more
europepmc +2 more sources