Results 31 to 40 of about 26,854 (254)

Pathogen quantitative efficacy of different spike-in internal controls and clinical application in central nervous system infection with metagenomic sequencing

Microbiology Spectrum, 2023
Metagenomic next-generation sequencing (mNGS) has been a lack of method for pathogen quantitation. We explored the suitable concentration of T1 phage [internal control (IC)phage], Thermus thermophilus (ICT.T), and artificial DNA sequence (ICDNA) as mNGS ...
Zhangfan Fu, Jingwen Ai, Haocheng Zhang, Peng Cui, Tao Xu, Yumeng Zhang, Yi Zhang, Honglong Wu, Ao Shen, Ke Lin, Miaoqu Zhang, Chao Qiu, Ning Jiang, Yang Zhou, Wenhong Zhang   +14 more
doaj   +1 more source

Development of a new gene expression vector for Thermus thermophilus using a silica-inducible promoter

Microbial Cell Factories, 2020
Background Thermostable enzymes are commonly produced in mesophilic hosts for research and bioengineering purposes. However, these hosts do not overexpress the active forms of some biologically functional thermoenzymes.
Yasuhiro Fujino, Shuichiro Goda, Yuri Suematsu, Katsumi Doi   +3 more
doaj   +1 more source

Unconventional lateral gene transfer in extreme thermophilic bacteria [PDF]

, 2011
Conjugation and natural competence are two major mechanisms that explain the acquisition of foreign genes throughout bacterial evolution. In recent decades, several studies in model organisms have revealed in great detail the steps involved in such ...
Berenguer, José, Bricio, Carlos, César, Carolina Elvira, Littauer, Dereck, Van Heerden, Esta, Álvarez, Laura   +5 more
core   +4 more sources

Sequence of the hyperplastic genome of the naturally competent Thermus scotoductus SA-01

BMC Genomics, 2011
Background Many strains of Thermus have been isolated from hot environments around the world. Thermus scotoductus SA-01 was isolated from fissure water collected 3.2 km below surface in a South African gold mine.
Gounder Kamini, Brzuszkiewicz Elzbieta, Liesegang Heiko, Wollherr Antje, Daniel Rolf, Gottschalk Gerhard, Reva Oleg, Kumwenda Benjamin, Srivastava Malay, Bricio Carlos, Berenguer José, van Heerden Esta, Litthauer Derek   +12 more
doaj   +1 more source

Proton and cadmium adsorption by the archaeon Thermococcus zilligii: Generalising the contrast between thermophiles and mesophiles as sorbents [PDF]

, 2010
Adsorption by microorganisms can play a significant role in the fate and transport of metals in natural systems. Surface complexation models (SCMs) have been applied extensively to describe metal adsorption by mesophilic bacteria, and several recent ...
Bremer, Phil J., Burnett, Peta-Gaye G., Daughney, Christopher J., Heinrich, Hannah T. M., Hetzer, Adrian, McQuillan, A. James, Morgan, Hugh W., Weerts, Marjolein   +7 more
core   +2 more sources

A deeply branching thermophilic bacterium with an ancient acetyl-CoA pathway dominates a subsurface ecosystem [PDF]

, 2012
A nearly complete genome sequence of Candidatus ‘Acetothermum autotrophicum’, a presently uncultivated bacterium in candidate division OP1, was revealed by metagenomic analysis of a subsurface thermophilic microbial mat community.
A Stamatakis, A Teske, AK Kaster, AL Brioukhanov, Atsushi Toyoda, AY Mulkidjanian, B Boussau, D Wu, DJ Tobler, DS Kelley, E Biegel, E Biegel, E Pierce, EF DeLong, EG Nisbert, F Nikitin, FD Ciccarelli, G Proskurowski, G Wächtershäuser, Gab-Joo Chee, H Hirayama, H Kimura, H Noguchi, H Takami, H Takami, H Takami, Hideki Noguchi, Hideto Takami, I Uchiyama, I Uchiyama, Ikuo Uchiyama, JA Baross, Jack Anthony Gilbert, JB Corliss, JR Torre, K Takai, K Takai, K Takai, KC Costa, Ken Takai, M Toei, M Wu, M Wu, Masahira Hattori, MB Scott, MS Rappé, N Saitou, O White, P Hugenholtz, R Caspi, R Chenna, RF Say, RT Thauer, S Guindon, S Kato, Shinro Nishi, T Murata, T Nunoura, T Nunoura, T Sakiyama, T Shibuya, Takehiko Itoh, Takuro Nunoura, TM Lowe, V Müller, W Martin, W Martin, W Martin, Wataru Arai, Yoshihiro Takaki   +69 more
core   +4 more sources

Transformation of Thermus Species by Natural Competence

Bio-Protocol, 2016
Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent.
Alba Blesa, José Berenguer
doaj   +1 more source

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]

, 2013
DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Klostermeier, Dagmar, Linden, Martin H., Rudolph, Markus G., Steimer, Lenz, Wurm, Jan Philip, Wöhnert, Jens   +5 more
core   +1 more source

Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium

Nature Communications, 2020
Type IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly of pilin proteins. Here, Neuhaus et al. show that the bacterium Thermus thermophilus produces two forms of type IV pilus, differing in structure, protein ...
Alexander Neuhaus, Muniyandi Selvaraj, Ralf Salzer, Julian D. Langer, Kerstin Kruse, Lennart Kirchner, Kelly Sanders, Bertram Daum, Beate Averhoff, Vicki A. M. Gold   +9 more
doaj   +1 more source

General and Genomic DNA-Binding Specificity for the Thermus thermophilus HB8 Transcription Factor TTHB023

Biomolecules, 2020
Transcription factors are proteins that recognize specific DNA sequences and affect local transcriptional processes. They are the primary means by which all organisms control specific gene expression.
James Shell Cox, Michael W. Van Dyke
doaj   +1 more source