Results 51 to 60 of about 26,854 (254)

The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8 [PDF]

, 2014
BACKGROUND: RNA metabolism, including RNA synthesis and RNA degradation, is one of the most conserved biological systems and has been intensively studied; however, the degradation network of ribonucleases (RNases) and RNA substrates is not fully ...
Akeo Shinkai, Hiromasa Ohyama, Kenji Fukui, Noriko Nakagawa, Ryoji Masui, Seiki Kuramitsu, Tomofumi Sakai, Yoshihiro Agari   +7 more
core   +1 more source

Cloning of the Thermus thermophilus methionine aminopeptidase gene and confirmation of the enzyme functional activity

Acta Biomedica Scientifica
Background. Methionine aminopeptidases (MAPs) are a class of enzymes that catalyze the removal of the N-terminal initiator methionine from a polypeptide chain.
V. V. Bykov, A. A. Vologzhannikova, M. V. Trunilina, T. A. Kudryashov, A. S. Sokolov, Yu. S. Lapteva   +5 more
doaj   +1 more source

Cryo-EM structure of the hibernating Thermus thermophilus 100S ribosome reveals a protein-mediated dimerization mechanism

Nature Communications, 2018
During stress conditions bacterial ribosomes dimerize and form inactive but stable hibernating 100S particles, a process that is facilitated by the hibernation-promoting factor (HPF).
Rasmus Kock Flygaard, Niels Boegholm, Marat Yusupov, Lasse B. Jenner   +3 more
doaj   +1 more source

Structural modelling of the complex of leucyl-tRNA synthetase and mis-aminoacylated tRNALeu [PDF]

, 2009
To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRS•tRNA ...
Hagiwara Yohsuke, Nureki Osamu, Tateno Masaru, 舘野 賢   +3 more
core   +1 more source

Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus

Journal of Lipid Research, 2006
The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses ...
Yu-Liang Yang, Feng-Ling Yang, Shu-Chuan Jao, Mao-Yen Chen, San-San Tsay, Wei Zou, Shih-Hsiung Wu   +6 more
doaj   +1 more source

Crystal structure of Thermus thermophilus methylenetetrahydrofolate dehydrogenase and determinants of thermostability.

PLoS ONE, 2020
The elucidation of mechanisms behind the thermostability of proteins is extremely important both from the theoretical and applied perspective. Here we report the crystal structure of methylenetetrahydrofolate dehydrogenase (MTHFD) from Thermus ...
Fernando Maiello, Gloria Gallo, Camila Coelho, Fernanda Sucharski, Leon Hardy, Martin Würtele   +5 more
doaj   +1 more source

Building Proteins in a Day: Efficient 3D Molecular Reconstruction

, 2015
Discovering the 3D atomic structure of molecules such as proteins and viruses is a fundamental research problem in biology and medicine. Electron Cryomicroscopy (Cryo-EM) is a promising vision-based technique for structure estimation which attempts to ...
Brubaker, Marcus A., Fleet, David J., Punjani, Ali   +2 more
core   +1 more source

Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases. [PDF]

, 2012
Some bacterial thermostable, wild-type or genetically engineered family A DNA polymerases have reverse transcriptase activity. However, difference in reverse transcriptase activities of family A DNA polymerases and retroviral reverse transcriptases (RTs)
Fujiwara, Shinsuke, Konishi, Atsushi, Nagaoka, Eriko, Shinomura, Mayu, Yasukawa, Kiyoshi   +4 more
core   +1 more source

A TtAgo‐Driven Autocatalytic Circuit with Thermal‐Enhanced Kinetics for One‐Pot Nucleic Acid Detection

Advanced Science, EarlyView.
This study proposes a universal catalytic DNA circuits termed TACTIC (Thermus thermophilus protein‐driven autocatalytic circuit) for one‐pot detection of DNA and RNA in multiple clinical samples. Integrated with machine learning, TACTIC accurately profile the distinct expression of four extracellular vesicle‐derived miRNAs across different samples and ...
Zuowei Xie, Ruijia Deng, Ben Niu, Yingjie Yang, Shuang Zhao, Hongzhao Yang, Meilin Gong, Jie Luo, Yu Tang, Jing Sheng, Yan Pi, Ming Chen, Kai Chang   +12 more
wiley   +1 more source

Advances in Double‐Stranded DNA Targeting Technologies

Exploration, EarlyView.
Double‐stranded DNA (dsDNA) plays a crucial role in genetic information storage and disease management, but its inherent stability limits access to internal bases. To overcome this challenge, various high‐specificity molecular targeting technologies have been developed to destabilize the DNA structure.
Zuhao Shen, Yiqun Liu, Yingjie Hao, Yifan Bo, Xiaochuan Dai, Shihui Wang, Tian Xia, Xin Su, Huiyu Liu   +8 more
wiley   +1 more source