Results 81 to 90 of about 40,909 (278)
A plasmid vector for an extreme thermophile,Thermus thermophilus [PDF]
FEMS Microbiology Letters, 1990 The host-vector system for an extreme thermophile, Thermus thermophilus HB27, was developed. The host strain has a mutation in tryptophan synthetase gene (trpB), and the mutation was determined to be a missense mutation by DNA sequence analysis. A Thermus-E. coli shuttle vector pYK109 was constructed.
K. Furukawa, Y. Koyama, Y. Arikawa
openaire +3 more sources
The heterotrimeric Thermus thermophilus Asp-tRNAAsn amidotransferase can also generate Gln-tRNAGln [PDF]
, 2000 Thermus thermophilus strain HB8 is known to have a heterodimeric aspartyl-tRNAAsn amidotransferase (Asp-AdT) capable of forming Asn-tRNAAsn [Becker, H.D. and Kern, D. (1998) Proc. Natl. Acad. Sci. USA 95, 12832–12837].
Becker, Hubert D. +8 more
core +1 more source
Electrostatic potential as a reactivity scoring function in computer‐assisted enzyme engineering
The FEBS Journal, EarlyView.We present a computational metric that quantifies the electrostatic complementarity of enzymes to the charge distribution of substrates at the transition state. Implemented in BindScan, this metric accurately predicts functionally relevant mutations in glycoside hydrolases.
Aitor Vega, Antoni Planas, Xevi Biarnés
wiley +1 more source
Identity elements of Thermus thermophilus tRNAThr [PDF]
FEBS Letters, 1996 In this study, we identified nucleotides that specify aminoacylation of tRNAThr by Thermus thermophilus threonyl‐tRNA synthetase (ThrRS) using in vitro transcripts. Mutation studies showed that the first base pair in the acceptor stem as well as the second and third positions of the anticodon are major identity elements of T.
Tsunemi Hasegawa +2 more
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Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1
MicrobiologyOpen, 2021 Several native and engineered heat‐stable DNA polymerases from a variety of sources are used as powerful tools in different molecular techniques, including polymerase chain reaction, medical diagnostics, DNA sequencing, biological diversity assessments ...
Ani Saghatelyan +3 more
doaj +1 more source
Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27 [PDF]
, 2017 There are 12 putative toxin–antitoxin (TA) loci in the Thermus thermophilus HB27 genome, including four VapBC and three HicBA families. Expression of these seven putative toxin genes in Escherichia coli demonstrated that one putative VapC toxin TTC0125 ...
Fan Yuqi +4 more
core +2 more sources
New Phytologist, Volume 246, Issue 5, Page 2084-2101, June 2025.Summary Flavodiiron proteins (FDPs) catalyse light‐dependent reduction of oxygen to water in photosynthetic organisms, creating an electron sink on the acceptor side of Photosystem I that protects the photosynthetic apparatus. However, ambiguity about the electron donor(s) remains, and the molecular mechanisms regulating FDP activity have remained ...
Lauri Nikkanen +9 more
wiley +1 more source
Structure of peptidoglycan from Thermus thermophilus HB8 [PDF]
Journal of Bacteriology, 1995 The composition and structure of peptidoglycan (murein) extracted from the extreme thermophilic eubacterium Thermus thermophilus HB8 are presented. The structure of 29 muropeptides, accounting for more than 85% of total murein, is reported. The basic monomeric subunit consists of N-acetylglucosamine-N-acetylmuramic acid-L-Ala-D-Glu-L-Orn-D-Ala-D-Ala ...
José Carlos Quintela +4 more
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Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR
Journal of Nucleic Acids, 2013 Thermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched primers. T.
Kenji Fukui, Seiki Kuramitsu
doaj +1 more source
Contribution of vesicle-protected extracellular DNA to horizontal gene transfer in Thermus spp. [PDF]
, 2016 Highly efficient apparatus for natural competence and conjugation have been shown as the major contributors to horizontal gene transfer (HGT) in Thermus thermophilus.
Alba Blesa, José Berenguer
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