Results 81 to 90 of about 26,854 (254)

Search for enzimes of interest in a library of metagenomics of hot spring : Beta-galactosidases [PDF]

, 2016
[Resumen] La obtención de β-galactosidasas a partir de metagenotecas de aguas termales, es de especial interés debido a que conservan su actividad a altas temperaturas y son más resistentes que sus homólogas mesófilas, permitiendo llevar a cabo la ...
García Marnotes, Natalí
core  

Nuclear Photosynthetic Gene Expression Is Synergistically Modulated by Rates of Protein Synthesis in Chloroplasts and Mitochondria [PDF]

, 2006
Arabidopsis thaliana mutants prors1-1 and -2 were identified on the basis of a decrease in effective photosystem II quantum yield. Mutations were localized to the 5'-untranslated region of the nuclear gene PROLYL-tRNA SYNTHETASE1 (PRORS1), which acts in ...
Bhushan, Shashi, Braun, Hans-Peter, Eubel, Holger, Glaser, Elzbieta, Leister, Dario, Masiero, Simona, Pesaresi, Paolo, Salamini, Francesco   +7 more
core   +2 more sources

Structural and functional characterization of a metagenomically derived γ‐type carbonic anhydrase and its engineering into a hyperthermostable esterase

Protein Science, Volume 34, Issue 12, December 2025.
Abstract The 16S microbial community profiling of a metagenomics library from geothermal spring at Lisvori (Lesvos island, Greece) enabled the identification of a putative sequence exhibiting 95% identity to the γ‐type carbonic anhydrase (γ‐CA) from Caloramator australicus (γ‐CaCA).
Charoutioun S. Bodourian, Mohsin Imran, Nikolaos D. Georgakis, Anastassios C. Papageorgiou, Nikolaos E. Labrou   +4 more
wiley   +1 more source

Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis [PDF]

, 2005
Protein structures from the causative agent of anthrax (Bacillus anthracis) are being determined as part of a structural genomics programme. Amongst initial candidates for crystallographic analysis are enzymes involved in nucleotide biosynthesis, since ...
Blagova, E V, Brannigan, J A, Fogg, M J, Grenha, R, Levdikov, V M, Wilkinson, A.J., Wilson, K S   +6 more
core   +3 more sources

Enhancing Allosteric Inhibition in Thermus thermophilus Phosphofructokinase [PDF]

Biochemistry, 2015
The coupling between the binding of the substrate Fru-6-P and the inhibitor phospho(enol)pyruvate (PEP) in phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus is much weaker than that seen in a PFK from Bacillus stearothermophilus.
McGresham, Maria S., Reinhart, Gregory D.   +1 more
openaire   +2 more sources

Supervised Learning of Protein Melting Temperature: Cross‐Species vs. Species‐Specific Prediction

Proteins: Structure, Function, and Bioinformatics, Volume 93, Issue 12, Page 2158-2166, December 2025.
ABSTRACT Protein melting temperatures are important proxies for stability, and frequently probed in protein engineering campaigns, for instance for enzyme discovery and protein optimization. With the emergence of large datasets of melting temperatures for diverse natural proteins, it has become possible to train models to predict this quantity, and the
Sebastián García López, Jesper Salomon, Wouter Boomsma   +2 more
wiley   +1 more source

Genome-scale metabolic network reconstruction and in silico flux analysis of the thermophilic bacterium Thermus thermophilus HB27 [PDF]

, 2014
10.1186/1475-2859-13-61Microbial Cell Factories131 ...
Aggarwal, S., Karimi, I.A., Lakshmanan, M., Lee, D.-Y., Lee, N.-R., Park, J.-B., Song, J.-W.   +6 more
core   +2 more sources

Substitution of CH3 by CH2F in 2‐Methylerythritol Cyclodiphosphate Triggers Potent Inhibition of IspG with Concomitant Fluoride Ion Expulsion

Chemistry – A European Journal, Volume 31, Issue 66, November 25, 2025.
The fluoromethyl analogue of MEcPP was identified as a very potent inhibitor of the oxygen‐sensitive [4Fe‐4S] metalloenzyme IspG. In the course of the IspG‐catalyzed transformation, this novel compound undergoes fluoride elimination, offering additional evidence for the involvement of a carbanionic intermediate in the mechanism of this enzyme ...
Clea Witjaksono, Vivien Herrscher, Hannah Jobelius, Nathan Noël, Fabien Massicot, Jean‐Luc Vasse, Jean‐Bernard Behr, Myriam Seemann   +7 more
wiley   +1 more source

Identification of signature proteins that are distinctive of the Deinococcus-Thermus phylum [PDF]

, 2010
The members of the Deinococcus-Thermus phylum, which include many species that are resistant to extreme radiation, as well as several thermophiles, have been recognized solely on the basis of their branching patterns in 16S rRNA and other phylogenetic ...
Emma Griffiths, Radhey S. Gupta
core   +2 more sources

Identity elements of Thermus thermophilus tRNA^Thr [PDF]

FEBS Letters, 1996
In this study, we identified nucleotides that specify aminoacylation of tRNAThr by Thermus thermophilus threonyl‐tRNA synthetase (ThrRS) using in vitro transcripts. Mutation studies showed that the first base pair in the acceptor stem as well as the second and third positions of the anticodon are major identity elements of T.
Nameki, Nobukazu, Asahara, Haruichi, Hasegawa, Tsunemi   +2 more
openaire   +2 more sources