Techniques for RNA extraction from cells cultured in starPEG–heparin hydrogels
Three-dimensional (3D) cell culture models that provide a biologically relevant microenvironment are imperative to investigate cell–cell and cell–matrix interactions in vitro.
Anna Jaeschke +6 more
doaj +1 more source
QUANTITATIVE REPRESENTATION OF THREE-DIMENSIONAL CELL CULTURE MODELS [PDF]
Three-dimensional mammary cell culture models offer new opportunities for the development of computational techniques for segmentation, localization, and multicellular organization. Under normal conditions, these assays form a symmetrical, hollow structure, which is necessary for their functional operation.
Chang, Hang +2 more
openaire +2 more sources
Progress in the application of ovarian and fallopian tube organoids
As an innovative in vitro culture model, organoids have been established by cell sorting and subsequent culture in three-dimensional culture systems.
Yi-Lin Dai +6 more
doaj +1 more source
Microfluidic Invasion Chemotaxis Platform for 3D Neurovascular Co-Culture
Advances in microfabrication and biomaterials have enabled the development of microfluidic chips for studying tissue and organ models. While these platforms have been developed primarily for modeling human diseases, they are also used to uncover cellular
Emel Sokullu +5 more
doaj +1 more source
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds [PDF]
A useful technique for culturing cells in a self-assembling nanofiber three-dimensional (3D) scaffold is described. This culture system recreates an environment that closely mimics the structural features of non-polarized tissue. Furthermore, the particular intrinsic nanofiber structure of the scaffold makes it transparent to visual light, which allows
Betriu, Nausika +2 more
openaire +2 more sources
From crypts to enteroids: establishment and characterization of avian intestinal organoids
: Intestinal organoids (IO), known as “mini-guts”, derived from intestinal crypts, are self-organizing three-dimensional (3D) multicellular ex vivo models that recapitulate intestine epithelial structure and function and have been widely used for ...
Dan Zhao +7 more
doaj +1 more source
Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion [PDF]
Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that
Hodgson, Lorna +3 more
core +2 more sources
Advances in Stem Cell Therapy Based on Tissue Engineering in the Treatment of Female Infertility with a Focus on POF [PDF]
Background: In the last few decades, many studies have been done on the treatment of premature ovarian failure. This review was conducted to study different types of treatment with a focus on the 3D culture model of stem cells as a pluripotent source for
Shadan Navid +5 more
doaj +1 more source
Two-Dimensional and Three-Dimensional Cell Culture and Their Applications
Cell culture is one of the most important and commonly used in vitro tools to comprehend various aspects of cells or tissues of a living body such as cell biology, tissue morphology, mechanism of diseases, cell signaling, drug action, cancer research and
Sangeeta Ballav +4 more
semanticscholar +1 more source
Three-Dimensional Culture Alters Primary Cardiac Cell Phenotype [PDF]
The directed formation of complex three-dimensional (3D) tissue architecture is a fundamental goal in tissue engineering and regenerative medicine. The growth of cells in 3D structures is expected to influence cellular phenotype and function, especially relative cell distribution, expression profiles, and responsiveness to exogenous signals; however ...
Robert E, Akins +5 more
openaire +2 more sources

