Results 81 to 90 of about 828,765 (377)

Determination of the rheological properties of Matrigel for optimum seeding conditions in microfluidic cell cultures

AIP Advances, 2018
Hydrogels are increasingly used as a surrogate extracellular matrix in three-dimensional cell culture systems, including microfluidic cell culture. Matrigel is a hydrogel of natural origin widely used in cell culture, particularly in the culture of stem ...
K. I. W. Kane, E. Lucumi Moreno, C. M. Lehr, S. Hachi, R. Dannert, R. Sanctuary, C. Wagner, R. M. T. Fleming, J. Baller   +8 more
doaj   +1 more source

Peptide hydrogels – versatile matrices for 3D cell culture in cancer medicine

Frontiers in Oncology, 2015
Traditional two-dimensional (2D) cell culture systems have contributed tremendously to our understanding of cancer biology but have significant limitations in mimicking in vivo conditions such as the tumor microenvironment.
Peter eWorthington, Peter eWorthington, Darrin John Pochan, Sigrid A Langhans   +3 more
doaj   +1 more source

Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion) [PDF]

, 2007
The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds.
Früh, Jennifer-Annika, Heberer, Michael, Jakob, Marcel, Martin, Ivan, Scherberich, Arnaud, Timmins, Nicholas E.   +5 more
core  

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

E-cadherin, actin, microtubules and FAK dominate different spheroid formation phases and important elements of tissue integrity

Biology Open, 2019
Spheroids resemble features of tissues and serve as model systems to study cell–cell and cell–ECM interactions in non-adhesive three-dimensional environments.
I. Smyrek, B. Mathew, S. C. Fischer, S. M. Lissek, S. Becker, E. H. K. Stelzer   +5 more
doaj   +1 more source

Design and functional testing of a multichamber perfusion platform for three-dimensional scaffolds [PDF]

Perfusion culture systems are widely used in tissue engineering applications for enhancing cell culture viability in the core of three-dimensional scaffolds.
Cantini, Marco, Ferrario, Giulio, Fiore, Gianfranco B., Piola, Marco, Sadr, Nasser, Soncini, Monica   +5 more
core   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Establishment of patient-derived three-dimensional organoid culture in renal cell carcinoma [PDF]

Investigative and Clinical Urology, 2020
Purpose: Renal cell carcinoma is a heterogeneous kidney cancer, and over 403,000 cases were reported worldwide in 2018. Current methods for studying renal cell carcinoma are limited to two-dimensional (2D) culture of primary cell lines and patient ...
Joon Chae Na , Jee-Hoon Kim , Sook Young Kim , Young-Ran Gu , Dae-Young Jun , Hyung Ho Lee , Young Eun Yoon , Kyung Hwa Choi , Sung Joon Hong , Woong Kyu Han   +9 more
doaj   +1 more source

Osteocyte isolation and culture methods. [PDF]

, 2016
The aim of this paper is to present several popular methods for in vitro culture of osteocytes and osteocyte cell lines. Osteocytes are located extremely suitably within the calcified bone matrix to sense mechanical signals, and are equipped with a ...
Bakker, A.D., Bravenboer, N., Pathak, J.L., Shah, K.M., Stern, A.R., Stern, M.M.   +5 more
core   +1 more source

Cytoplasmic p21 promotes stemness of colon cancer cells via activation of the NFκB pathway

Molecular Oncology, EarlyView.
Cytoplasmic p21 promotes colorectal cancer stem cell (CSC) features by destabilizing the NFκB–IκB complex, activating NFκB signaling, and upregulating BCL‐xL and COX2. In contrast to nuclear p21, cytoplasmic p21 enhances spheroid formation and stemness transcription factor CD133.
Arnatchai Maiuthed, Kerstin Huebner, Katharina Erlenbach‐Wuensch, Chuanpit Hampel, Daniela Thalheim, Adriana Vial‐Roehe, Bodee Nutho, Susanne Merkel, Arndt Hartmann, Pithi Chanvorachote, Regine Schneider‐Stock   +10 more
wiley   +1 more source