Results 101 to 110 of about 20,905,469 (291)

Hydrofocusing Bioreactor for Three-Dimensional Cell Culture [PDF]

, 2003
The hydrodynamic focusing bioreactor (HFB) is a bioreactor system designed for three-dimensional cell culture and tissue-engineering investigations on orbiting spacecraft and in laboratories on Earth. The HFB offers a unique hydrofocusing capability that
Gonda, Steve R., Tsao, Yow-Min D., Jones, Leslie, Spaulding, Glenn F., Flechsig, Scott, Soehnge, Holly   +5 more
core  

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

FEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito, Haduki Asano, Ayano Ueki, Joe Sakamoto, Yasuhiro Kamei, Hayato Tokumoto, Ayaka Yazawa, Shigeki Kamitani   +7 more
wiley   +1 more source

Assessing Receptor Activation in 2D and 3D Cultured Hepatocytes: Responses to a Single Compound and a Complex Mixture

Toxics
Responding to global standards and legislative updates in Canada, including Bill S-5 (2023), toxicity testing is shifting towards more ethical, in vitro methods. Traditional two-dimensional (2D) monolayer cell cultures, limited in replicating the complex
Laiba Jamshed, Shanza Jamshed, Richard A. Frank, L. Mark Hewitt, Philippe J. Thomas, Alison C. Holloway   +5 more
doaj   +1 more source

Three-dimensional hydrogel cell culture systems for modeling neural tissue [PDF]

, 2009
Two-dimensional (2-D) neural cell culture systems have served as physiological models for understanding the cellular and molecular events that underlie responses to physical and chemical stimuli, control sensory and motor function, and lead to the ...
Frampton, John P.
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Three-Dimensional Culture of Glioblastoma Cells Using a Tissueoid Cell Culture System

ACTA HISTOCHEMICA ET CYTOCHEMICA
In classical cell culture techniques, cancer cells typically proliferate in a single layer by adhering to the undersurface of laboratory vessels. Consequently, concerns have been raised regarding the fidelity of the morphological and functional characteristics of these cultured cancer cells compared to those of their in vivo counterparts.
Okamoto, Natsume, Taniura, Naoko, Nakayama, Takahisa, Tanaka, Eri, Kageyama, Yusuke, Noujima, Mai, Kushima, Ryoji, Mukaisho, Ken-ichi   +7 more
openaire   +2 more sources

Reduced-dimensional models for straight-channel proton exchange membrane fuel cells [PDF]

, 2010
A comprehensive description of proton exchange membrane fuel cell (PEMFC) performance includes the transport phenomena, phase change and electrochemical reaction inside the several components, which possess disparate characteristics and together form a ...
Sui, P.C., Shah, Akeel A., Shah, A.A., Sui, P. C., Kim, Gwang-Soo, Djilali, Ned   +5 more
core   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Establishment of an Three-Dimensional Vascularized Micro-Tumor Model and Screening of Chemotherapeutic Drugs

Technology in Cancer Research & Treatment
Breast cancer is the most common malignancy in women worldwide, and major challenges in its treatment include drug resistance and metastasis. Three-dimensional cell culture systems have received widespread attention in drug discovery studies but existing
Qian Ye MD, Juanru Wang BS, Boyang Wang BS, Min Zhao BS, Zhengsheng Wu PhD, Xiaoli Liu PhD   +5 more
doaj   +1 more source