Results 121 to 130 of about 20,905,469 (291)

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Multifunctional microgel stent accelerates wound healing

Nano Research
In the field of wound healing, hydrogels have garnered significant attention due to their outstanding biocompatibility and versatile functionality.
Boxiong Bai, Zhisheng Xiao, Zhaoxin Ji, Qiang Zhang, Jiafei Zhu, Nanhui Liu, Xinying Lv, Yifan Yang, Pengxing Li, Xianglei Wu, Qian Chen   +10 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

FEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi, Alain Dolla, Frauke Baymann, Celine Rommevaux, Latifa Elantak, Helene Gaussier, Matthieu Nouailler   +6 more
wiley   +1 more source

Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture

, 2018
A well-organised vascular network is essential for metabolic exchange to maintain homoeostasis in the body. Therefore, for progress in regenerative medicine, it is particularly important to establish methods of vascularization in bioengineered three ...
Tatsuya Shimizu, Shinako Masuda, Katsuhisa Matsuura   +2 more
core   +1 more source

Determinants of undergraduate bioscience engagement: Validating attendance barriers and classroom spatial behaviour across institutions

FEBS Open Bio, EarlyView.
Student engagement extends beyond simple attendance. Across 891 bioscience students from three universities, attendance behaviour was associated mainly with structural influences, whereas classroom spatial behaviour reflected affective and sensory factors.
Nigel Page, Rachel J. Hunt, Lauren J. Albee, Daniel Berwick, Alison Snape   +4 more
wiley   +1 more source

Integrated PANoptosis Profiling Identifies Immunosuppressive Subtypes and a Prognostic Signature With Functional Validation of MLKL in Glioblastoma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian, Minghui Zhao, Yuanbo Hu, Kaiyue Wang, Haiguang Liu, Zetong Bai, Kebin Zheng   +6 more
wiley   +1 more source

Quantification of collagen contraction in three-dimensional cell culture

, 2015
Many different cell types including fibroblasts, smooth muscle cells, endothelial cells, and cancer cells exert traction forces on the fibrous components of the extracellular matrix. This can be observed as matrix contraction both macro- and microscopically in three-dimensional (3D) tissues models such as collagen type I gels.
Kopanska, Katarzyna S., Bussonnier, Matthias, Geraldo, Sara, Simon, Anthony, Vignjevic, Danijela, Betz, Timo, Paluch, Ewa K.   +6 more
openaire   +3 more sources

Three-dimensional co-culturing reveals human stem cell-derived somatostatin interneurons with subclass expression

Cortical interneuron deficiencies, particularly involving the somatostatin (SST) subtypes, contribute to neurological and neuropsychiatric disorders. These interneurons are difficult to derive in vitro from human embryonic stem cells (hESCs) due to their
Bruzelius, Andreas, Olariu, Victor, Stamouli, Christina Anastasia, Passarello, Germán Ramos, Aretio-Medina, Constanza, Cepeda-Prado, Efrain, Sozzi, Edoardo, Giacomoni, Jessica, Rylander Ottosson, Daniella, Nocera, Gianluigi, Hölldobler, Anna Lena, Giacomoni, Jessica,, Nocera, Gianluigi,, Olariu, Victor,, Hölldobler, Anna Lena,, Bruzelius, Andreas,, Cepeda-Prado, Efrain,, Passarello, Germán Ramos,, Sozzi, Edoardo,, Rylander Ottosson, Daniella,, Aretio-Medina, Constanza,, Stamouli, Christina Anastasia,, Lund University.   +22 more
core   +1 more source

Posterior Disconnection Syndrome in Early‐Stage Adult‐Onset Cerebral Adrenoleukodystrophy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Adult‐onset cerebral adrenoleukodystrophy is potentially treatable but often difficult to recognize before advanced cerebral involvement. Herein, we describe three men with early‐stage disease who initially presented with subtle visual complaints rather than subcortical dementia. Targeted neuropsychological testing revealed higher‐order visual
Kazuto Katsuse, Takashi Matsukawa, Yumiko Nakamura, Kazuo Kakinuma, Shoko Ota, Ryo Hara, Saki Nakashima, Kaoru Ito, Yutaro Nakamori, Toshiyuki Kakumoto, Masashi Hamada, Shigenori Kanno, Kyoko Suzuki, Tatsushi Toda   +13 more
wiley   +1 more source