Results 91 to 100 of about 20,905,469 (291)

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds

, 2007
A mechanistic understanding of adipose tissue differentiation is critical for the treatment and prevention of obesity and type 2 diabetes. Conventional in vitro models of adipogenesis are preadipocytes or freshly isolated adipocytes grown in two ...
Xie, Yubing, Lee, L. James, Douglas A. Kniss, Powell, Heather M., Kang, Xihai, Lannutti, John J., Kniss, Douglas A., Belury, Martha A.   +7 more
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Extracellular vesicle miRNAs from three-dimensional ovarian cancer in vitro models and their implication in overall cancer survival

Heliyon
Ovarian cancer is the most common gynaecological malignancy and the seventh most diagnosed cancer in females worldwide. Currently, it is the sixth leading cause of cancer related mortality among patients globally.
Nihar Godbole, Andrew Lai, Flavio Carrion, Katherin Scholz-Romero, Akhilandeshwari Ravichandran, Priyakshi Kalita-de Croft, Amy E. McCart Reed, Vaibhavi Joshi, Sunil R. Lakhani, Mostafa Kamal Masud, Yusuke Yamauchi, Lewis Perrin, John Hooper, Laura Bray, Dominic Guanzon, Carlos Salomon   +15 more
doaj   +1 more source

POLYSACCHARIDE-BASED SHEAR THINNING HYDROGELS FOR THREE-DIMENSIONAL CELL CULTURE [PDF]

, 2015
The recreation of the complicated tissue microenvironment is essential to reduce the gap between in vitro and in vivo research. Polysaccharide-based hydrogels form excellent scaffolds to allow for three-dimensional cell culture owing to the favorable ...
Surampudi, Vasudha
core   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Low-temperature culturing improves survival rate of tissue-engineered cardiac cell sheets

Biochemistry and Biophysics Reports, 2018
Assembling three-dimensional (3D) tissues from single cells necessitates the use of various advanced technological methods because higher-density tissues require numerous complex capillary structures to supply sufficient oxygen and nutrients. Accordingly,
Katsuhisa Sakaguchi, Yuto Hinata, Yuki Kagawa, Kiyotaka Iwasaki, Satoshi Tsuneda, Tatsuya Shimizu, Mitsuo Umezu   +6 more
doaj   +1 more source

Three-dimensional cell culture can be regulated by vibration: low-frequency vibration increases the size of olfactory ensheathing cell spheroids

, 2019
Background: Olfactory ensheathing cell (OEC) transplantation is emerging as a promising therapy for spinal cord injuries. However, outcomes are inconsistent, and the method needs improvement.
Todorovic, Michael, Beckingham, Lachlan J, Chen, Mo, Velasquez, Johana Tello, Ekberg, Jenny AK, St John, James A, Vial, Marie-Laure   +6 more
core   +1 more source

Vascularization in 3D Cell Culture

, 2021
The two-dimensional (2D) monoculture does not recapitulate the three-dimensional (3D) environment of in vivo vasculature. Endothelial cell (ECs) spheroids have been introduced as a 3D in vitro model exhibiting angiogenic responses and sprouting behavior ...
Bagli, E., Murphy, C., D. Kouroupis, E. Bagli, M. Markou, Kouroupis, D., C. Murphy, Fotsis, T., Markou, M., T. Fotsis   +9 more
core   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source