Results 121 to 130 of about 18,631,052 (336)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Editorial: Three-dimensional/3D stem cell culture systems

Frontiers in Cell and Developmental Biology, 2023
Katiucia Batista Silva Paiva, José Mauro Granjeiro, José Mauro Granjeiro, Leandra Santos Baptista, Alexandra P. Marques, Alexandra P. Marques, Ana Rosa Ribeiro, Elizaveta Koudan   +7 more
doaj   +1 more source

Extracellular vesicle miRNAs from three-dimensional ovarian cancer in vitro models and their implication in overall cancer survival

Heliyon
Ovarian cancer is the most common gynaecological malignancy and the seventh most diagnosed cancer in females worldwide. Currently, it is the sixth leading cause of cancer related mortality among patients globally.
Nihar Godbole, Andrew Lai, Flavio Carrion, Katherin Scholz-Romero, Akhilandeshwari Ravichandran, Priyakshi Kalita-de Croft, Amy E. McCart Reed, Vaibhavi Joshi, Sunil R. Lakhani, Mostafa Kamal Masud, Yusuke Yamauchi, Lewis Perrin, John Hooper, Laura Bray, Dominic Guanzon, Carlos Salomon   +15 more
doaj   +1 more source

A spheroid-based 3-D culture model for pancreatic cancer drug testing, using the acid phosphatase assay

Brazilian Journal of Medical and Biological Research, 2013
Current therapy for pancreatic cancer is multimodal, involving surgery and chemotherapy. However, development of pancreatic cancer therapies requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent ...
Z. Wen, Q. Liao, Y. Hu, L. You, L. Zhou, Y. Zhao   +5 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Development of a Compact Microbubble Generator and Its Usefulness for Three-Dimensional Osteoblastic Cell Culture

Journal of Biomechanical Science and Engineering, 2007
Three-dimensional cell culture is widely used for challenging to biomedical tissue reconstruction. A mass of the reconstructed tissue has been limited because of difficulty in supplying sufficient amount of oxygen deep into the densely cultured cells ...
Kosaku KURATA, Hiroyasu TANIGUCHI, Takanobu FUKUNAGA, Junpei MATSUDA, Hidehiko HIGAKI   +4 more
doaj   +1 more source

Hydrofocusing Bioreactor for Three-Dimensional Cell Culture [PDF]

, 2003
The hydrodynamic focusing bioreactor (HFB) is a bioreactor system designed for three-dimensional cell culture and tissue-engineering investigations on orbiting spacecraft and in laboratories on Earth. The HFB offers a unique hydrofocusing capability that
Gonda, Steve R., Tsao, Yow-Min D., Jones, Leslie, Spaulding, Glenn F., Flechsig, Scott, Soehnge, Holly   +5 more
core  

Three-dimensional reconstruction of surface nanoarchitecture from two-dimensional datasets

, 2014
The design of biomaterial surfaces relies heavily on the ability to accurately measure and visualize the three-dimensional surface nanoarchitecture of substrata.
Hayden Webb (18925450), Russell Crawford (18943777), Veselin Boshkovikj (18998849), Vy Pham (18942868), Elena Ivanova (18926545), Christopher Fluke (18925279)   +5 more
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source