Results 81 to 90 of about 162,133 (299)

Stabilizing Functional Nucleic Acids for Real‐Sample Diagnostics

Angewandte Chemie, EarlyView.
Functional nucleic acids enable programmable real‐sample diagnostics, but often lose function in complex biological matrices. This review highlights four stabilization levers—chemical, topological, architectural, and microenvironment/interface engineering—that preserve aptamer and DNAzyme performance in saliva, serum, urine, and stool, reframing ...
Soyeon Lee, Letizia Dondi, Amal Mathai, Connor Nurmi, Yingfu Li   +4 more
wiley   +2 more sources

Inhibition of MEKK3 Prevents Cerebral Cavernous Malformation Progression Via Restoring Endothelial Integrity and Restricting Microglial Activation

Advanced Science, EarlyView.
In this study, we demonstrate that aberrant MEKK3 signaling in endothelial cells and microglia drives the progression of cerebral cavernous malformations (CCMs). Furthermore, we identify DPDH as a potent small‐molecule MEKK3 inhibitor with substantial therapeutic potential for CCM treatment.
Weiwei Zheng, Suyun Yu, Liwenyu Chen, Jing Ma, Peiliang Shen, Chang Yu, Xiaoqing Xin, Qiuhong Shen, Jing Chen, Song Ke, Hao Liu, Xiaobing Jiang, Ruogu Qi, Juming Yan, Jia Li, Jaesung P. Choi, Yin Lu, Yang Zhao   +17 more
wiley   +1 more source

PPACK-thrombin is a noncompetitive inhibitor of [alpha]-thrombin binding to human platelets

, 1992
Recent studies from our laboratory indicate that purified kininogens are noncompetitive inhibitors of human [alpha]-thrombin but not PPACK-thrombin, binding to human washed platelets.
Meloni, F. J., Nawarawong, W., Schmaier, Alvin H., Jiang, Y. P.   +3 more
core   +1 more source

A Biomimetic cRGDyk‐Engineered Platelet Nanoplatform for ACAD11 Delivery to Trigger Pyroptosis in Hepatocellular Carcinoma

Advanced Science, EarlyView.
A biomimetic platelet nanoplatform (OE‐ACAD11@R‐PLT) is developed for targeted hepatocellular carcinoma immunotherapy. By selectively delivering therapeutic plasmids, this system induces mitochondrial dysfunction and triggers robust NLRP3/Caspase‐1/GSDMD‐mediated pyroptosis in tumor cells.
Hongyi Bao, Wei Feng, Wei Zong, Yi Li, Xinyue Qin, Xinyue Wang, Yubo Yang, Guanhua Chen, Yiting Hou, Xuan Tan, Lin Chen, Ming Zhang, Feng Wang   +12 more
wiley   +1 more source

Extending Small‐Molecule Aptamer Discovery Principles to Protein Targets: Selection and Characterization of Green Fluorescent Protein Aptamers

Angewandte Chemie, EarlyView.
Protein‐binding aptamers remain poorly understood compared to their small‐molecule counterparts. Using green fluorescent protein (GFP) variants as a tractable model system, we show how subtle changes in protein surface composition and divalent metal ions reshape aptamer selection, specificity, and binding.
Stefen Stangherlin, Nathan Wong, Logan Fanning, Kate Kim, Peter Kim, Iffat Tabassum, Songyang Miao, Rui Huang, Juewen Liu   +8 more
wiley   +2 more sources

The Coagulation Factors Fibrinogen, Thrombin, and Factor XII in Inflammatory Disorders—A Systematic Review

Frontiers in Immunology, 2018
BackgroundThe interaction of coagulation factors has been shown to go beyond their traditional roles in hemostasis and to affect the development of inflammatory diseases.
Kerstin Göbel, Susann Eichler, Heinz Wiendl, Triantafyllos Chavakis, Christoph Kleinschnitz, Sven G. Meuth   +5 more
doaj   +1 more source

Boophilin inhibits thrombin.

, 2016
(A) Typical progress curves for thrombin-mediated hydrolysis of S2238 in the absence and presence of Boophilin at the indicated concentrations (a, 0 nM; b, 20 nM; c; 40 nM; d, 80 nM; e, 160 nM; f, 320 nM). Reactions started with addition of S2238 (250 μM)
Dongying Ma (132065), Michail Kotsyfakis (181487), Daniella M. Mizurini (628533), Robson Q. Monteiro (232081), Teresa C. Assumpção (258093), Ivo M. B. Francischetti (114544), José M. C. Ribeiro (123592), R. Manjunatha Kini (124799)   +7 more
core   +1 more source

Engineering a Bioelectronic Hybrid Heart with Integrated Sensing and Electrical Modulation

Advanced Science, EarlyView.
A bioelectronic hybrid heart is engineered by integrating iPSC‐derived cardiomyocytes, a decellularized cardiac scaffold, a cell‐laden ECM coating, and a flexible electronic mesh. The embedded electrodes enable real‐time recording of spontaneous electrical activity and localized stimulation, allowing controlled pacing and modulation of activation ...
Itai Cabilly, Hadas Oved, Ron Feiner, Nadav Noor, Moran Yadid, Melanie Flury, Assaf Shapira, Tal Dvir   +7 more
wiley   +1 more source

Time‐Resolved Multidimensional NMR Spectroscopy Reveals the Bifurcated Folding Pathway of a Thrombin‐Binding DNA G4–Azobenzene Derivative Complex

Angewandte Chemie, EarlyView.
A photoswitchable ligand was used to trigger conformational changes within the thrombin binding aptamer DNA G‐Quadruplex. These reversible ligand driven rearrangements were examined by time‐resolved NMR experiments to gain insight of the dynamic folding landscape of the G‐quadruplex.
Jason Martins, Julia Wirmer‐Bartoschek, Harald Schwalbe   +2 more
wiley   +2 more sources

Oxidative stress model of lipopolysaccharide combined with thrombin inducing in broiler chicks

Frontiers in Veterinary Science
Lipopolysaccharides (LPS) are commonly used to construct inflammation models. However, poultry have a certain degree of tolerance to LPS due to the lack of thrombin XI and XII in their bodies. Thrombin activation produces clotting factors that can cleave
Huiyu Shi, Fengyuan Yang, Zeru Peng, Junlong Wu, Qin Wang, Pingfei Qiu, Ruiying Bao, Liangmin Huang, Xiaochun Li, Haiwen Zhang, Xuemei Wang   +10 more
doaj   +1 more source