Results 81 to 90 of about 1,052,538 (294)

High-throughput technologies in polymer research Proceedings

, 2002
High-Throughput Experimentation (HTE) is a well established technique in the pharmaceutical and biotechnology industries leading to faster and less expensive development of new drugs and therapies.
Gesellschaft fuer Chemische Technik und Biotechnologie e.V. (DECHEMA), Frankfurt am Main (Germany)
core  

SPHINX31 acts as a SRPK1 inhibitor targeting the ATR/DNA‐PKcs/CHK1 replicative checkpoint to inhibit cell growth in non‐small cell lung cancer

Molecular Oncology, EarlyView.
The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim, Aurelie Genoux, Nadiia Zubchuk, Haoyang Zheng, Perla Salameh, Theo Ziegelmeyer, Fabien Dalonneau, Margot Rouchette, Christiane Oddou, Simona Miron, Helene Polveche, Adiilah Mamode Cassim, Carmen Garrido, Eleni Nikolakaki, Didier Auboeuf, Tao Jia, Sophie Zinn‐Justin, Beatrice Eymin   +17 more
wiley   +1 more source

High-Throughput Analysis of the Plasma N-Glycome by UHPLC

, 2016
The understanding of glycosylation alterations in health and disease has evolved significantly and glycans are considered to be relevant biomarker candidates.
O’Flaherty, R., Rudd, P. M., Henning Stöckmann, Pauline M. Rudd, Barbara Adamczyk, Karlsson, Niclas G.,, Stöckmann, H., Adamczyk, Barbara,, Niclas G. Karlsson, Róisín O’Flaherty   +9 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Mitochondria do not contain lipid rafts, and lipid rafts do not contain mitochondrial proteins[S]

Journal of Lipid Research, 2009
Lipid rafts are membrane microdomains involved in many cellular functions, including transduction of cellular signals and cell entry by pathogens.
Yu Zi Zheng, Kyra B. Berg, Leonard J. Foster   +2 more
doaj   +1 more source

High-Throughput Phenomics

, 2015
Standard protocols are available in order to apply Phenotype MicroArray (PM) technology to characterize different groups of microorganisms. Nevertheless, there is the need to pay attention to several crucial steps in order to obtain high-quality and reproducible data from PM, such as the choice of the Dye mix, the type and concentration of the carbon ...
Carlo, Viti, Francesca, Decorosi, Emmanuela, Marchi, Marco, Galardini, Luciana, Giovannetti   +4 more
openaire   +3 more sources

DS_DISC786509 – Supplemental material for Development and Characterization of Quantitative, High-Throughput-Compatible Assays for Proteolytic Degradation of Glucagon

, 2018
Supplemental material, DS_DISC786509 for Development and Characterization of Quantitative, High-Throughput-Compatible Assays for Proteolytic Degradation of Glucagon by Caitlin N. Suire, Shelley Lane and Malcolm A.
Malcolm A. Leissring (249235), Caitlin N. Suire (4857490), Shelley Lane (5512298)   +2 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Catalyst Optimization in Olefin Polymerization using an HTE/QSAR workflow: Intuitive 3D-Steric Descriptors to Tackle the Database Size Problem

, 2021
Catalyst screening in olefin polymerization is an inherently complex problem. Even using high-throughput experimentation (HTE) tools, databases are usually tiny (at least when compared to those in pharma), rarely comprised of more than several dozen ...
Roberta Cipullo, Georgy P. Goryunov, Peter H. M. Budzelaar, Dmitry V. Uborsky, Vyatcheslav V. Izmer, Antonio Vittoria, Christian Ehm, Mikhail I. Sharikov, Pavel S. Kulyabin, Vincenzo Busico, Alexander Z. Voskoboynikov   +10 more
core  

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source