Results 51 to 60 of about 9,379,767 (301)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Highlighting radical sites through polarized neutron scattering from AFP-modulated polarized protons

IUCrJ
Time-resolved neutron scattering has been used to study dynamically polarized protons in tyrosyl-doped bovine liver catalase. While the evolution of proton polarization and its inversion by the method of adiabatic fast passage (AFP) in a standard dynamic
Patrick Hautle, Oliver Zimmer, Hélène M. Jouve, Heinrich B. Stuhrmann   +3 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Photophysics and Photochemistry of Canonical Nucleobases’ Thioanalogs: From Quantum Mechanical Studies to Time Resolved Experiments

Molecules, 2017
Interest in understanding the photophysics and photochemistry of thiated nucleobases has been awakened because of their possible involvement in primordial RNA or their potential use as photosensitizers in medicinal chemistry.
Serra Arslancan, Lara Martínez-Fernández, Inés Corral   +2 more
doaj   +1 more source

Photophysical Studies of Poly(3,4-Ethylenedioxythiophene/Cucurbit[7]uril) Polypseudorotaxane and Polyrotaxane by Transient Absorption and Time-Resolved Fluorescence Spectroscopy

Engineering Proceedings, 2022
The UV-Vis absorption, fluorescence, and phosphorescence spectra of poly(3,4-ethylenedioxythiophene/cucurbit[7]uril), polypseudorotaxane (1), and polyrotaxane (2) in water and acetonitrile solutions were investigated. To achieve a deeper insight into the
Radu Ionut Tigoianu, Aurica Farcas
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Probing laser-driven surface and subsurface dynamics via grazing-incidence XFEL scattering and diffraction

IUCrJ
We demonstrate a grazing-incidence X-ray platform that simultaneously records time-resolved grazing-incidence small-angle X-ray scattering (GISAXS) and grazing-incidence X-ray diffraction (GID) from a femtosecond-laser-irradiated gold film above the ...
Lisa Randolph, Özgül Öztürk, Dmitriy Ksenzov, Lingen Huang, Thomas Kluge, S. V. Rahul, Victorien Bouffetier, Tobias Held, Sebastian T. Weber, Carsten Baehtz, Mohammadreza Banjafar, Erik Brambrink, Fabien Brieuc, Byoung Ick Cho, Sebastian Göde, Hauke Höppner, Gerhard Jakob, Mathias Kläui, Zuzana Konôpková, Changhoo Lee, Gyusang Lee, Mikako Makita, Mikhail Mishchenko, Mianzhen Mo, Pascal D. Ndione, Franziska Paschke-Bruehl, Michael Paulus, Alexander Pelka, Thomas R. Preston, Christian Rödel, Michal Šmíd, Ling Wang, Lennart Wollenweber, Baerbel Rethfeld, Jan-Patrick Schwinkendorf, Christian Gutt, Motoaki Nakatsutsumi   +36 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Time-Resolved and Dynamic Studies of Riboswitches [PDF]

Biophysical Journal, 2014
Riboswitches are a class of regulatory RNA molecules widely distributed mostly across prokaryotic organisms. The discovery of the first riboswitches, by the Breaker lab and others, opened the door to a previously unimagined layer of genetic regulation.
openaire   +1 more source