Results 71 to 80 of about 11,364 (306)

Timed Fast Exact Euclidean Distance (tFEED) maps

, 2005
In image and video analysis, distance maps are frequently used. They provide the (Euclidean) distance (ED) of background pixels to the nearest object pixel.
Kuppens, H., Laplante, Philip A., Schouten, T, Schouten, T.E., Laplante, P.A., Kuppens, H.C.W., van den Broek, Egon, Kehtarnavaz, Nasser, Kuppens, Harco, Kehtarnavaz, N., van den Broek, E.L., Schouten, Theo E., Broek, E.L. van den   +12 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The effects of gonadotrophin releasing hormone in prostaglandin F_2α-based timed insemination programs for beef cattle

, 2001
Trials were conducted in the spring (May; n = 324) and fall (October; n = 132) with crossbred continental-type beef cows assigned on the basis of parity and postpartum interval to one of three timed-AI treatments and one of two post-AI treatments.
N. D. Glover, W. P. McCaughey, J. A. Small, D. R. Ward, W. D. Sutherland, J. D. Ambrose, R. Rajamahendran   +6 more
core   +1 more source

Milk Metabolomics Reveals Potential Biomarkers for Early Prediction of Pregnancy in Buffaloes Having Undergone Artificial Insemination

Animals, 2020
This study aimed to identify potential biomarkers for early pregnancy diagnosis in buffaloes subjected to artificial insemination (AI). The study was carried out on 10 pregnant and 10 non-pregnant buffaloes that were synchronized by Ovsynch-Timed ...
Donato de Nicola, Francesco Vinale, Angela Salzano, Giada d’Errico, Anastasia Vassetti, Nunzia D’Onofrio, Maria Luisa Balestrieri, Gianluca Neglia   +7 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Comparison of the effects of two shortened timed-AI protocols on pregnancy per AI in beef cattle

, 2020
The objective was to compare pregnancy per AI (P/AI) between two shortened timed-AI (TAI) protocols in beef cattle. This study also determined whether administration of eCG in heifers and timing of AI in cows would affect P/AI.
Mapletoft, R.J., Colazo, M.G., Bignell, D., Boender, G., Macrae, L., Sanz, A., Macmillan, K., Gobikrushanth, M.   +7 more
core   +1 more source

The effect of progesterone length in timed AI in ewes

, 2022
This study aimed to evaluate the reproductive performance of ewes submitted to timed AI protocol with 7 (D7) or 9 (D9) days of progesterone. A total of 220 crossbred ewes (Doper × Santa Ines) were distributed in the treatments according to body weight ...
Marcos Vinicius de Castro Ferraz Junior, Barroso, José Paulo Roman, Evandro Maia Ferreira, Amanda Pimentel Sinimbu, Janaina Socolovski Biava, Pires, Alexandre Vaz, Sinimbu, Amanda Pimentel, Ferreira, Evandro Maia, José Paulo Roman Barroso, Renan Denadai, Alexandre Vaz Pires, Biava, Janaina Socolovski, Denadai, Renan [UNESP], de Castro Ferraz Junior, Marcos Vinicius   +13 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Ultrasonographic evaluation of endometrial thickness near timed AI as a predictor of fertility in high-producing dairy cows

, 2011
The objectives were to evaluate changes in endometrial thickness (ET) near the time of a synchronized ovulation and to assess the relationship of ET and fertility in lactating Holstein cows, with or without estrogen supplementation near timed ovulation ...
D.J. Brusveen, Cunha, Arcelina Pacheco, M.C. Wiltbank, Brusveen, D. J., A. Gümen, A.P. Cunha, J.N. Guenther, Souza, Alexandre H, Silva, Eduardo P.B., H. Ayres, A.H. Souza, E.P.B. Silva, Guenther, Jerry N., Ayres, Henderson, Wiltbank, Milo Charles   +14 more
core   +1 more source