Whole-brain vasculature reconstruction at the single capillary level [PDF]
, 2018 The distinct organization of the brain’s vascular network ensures that it is adequately supplied with oxygen and nutrients. However, despite this fundamental role, a detailed reconstruction of the brain-wide vasculature at the capillary level remains ...
Allegra Mascaro, Anna Letizia +8 more
core +2 more sources
BMC Biology, 2022 Background Tissue-clearing techniques have recently been developed to make tissues transparent for three-dimensional (3D) imaging at different scales, including single-cell resolution. However, current tissue-clearing workflows have several disadvantages,
Qing-Hong Shan +6 more
doaj +1 more source
Global Connectivity and Function of Descending Spinal Input Revealed by 3D Microscopy and Retrograde Transduction [PDF]
, 2018 The brain communicates with the spinal cord through numerous axon tracts that arise from discrete nuclei, transmit distinct functions, and often collateralize to facilitate the coordination of descending commands.
Blackmore, Murray G. +4 more
core +2 more sources
Optimized Protocol for Imaging Cleared Neural Tissues Using Light Microscopy [PDF]
, 2016 Understanding physical and chemical processes at an organismal scale is a fundamental goal in biology. While science is adept at explaining biological phenomena at both molecular and cellular levels, understanding how these processes translate to ...
Bergan, J +3 more
core +1 more source
Three-Dimensional Imaging of the Intracellular Fate of Plasmid DNA and Transgene Expression: ZsGreen1 and Tissue Clearing Method CUBIC Are an Optimal Combination for Multicolor Deep Imaging in Murine Tissues. [PDF]
PLoS ONE, 2016 Evaluation methods for determining the distribution of transgene expression in the body and the in vivo fate of viral and non-viral vectors are necessary for successful development of in vivo gene delivery systems.
Shintaro Fumoto +3 more
doaj +1 more source
Multimode fibre based imaging for optically cleared samples [PDF]
, 2017 UK Engineering and Physical Sciences Research Council (EPSRC) (EP/P030017/1); European Union’s Horizon 2020 Marie Skłodowska-Curie Actions (MSCA) (707084)Optical clearing is emerging as a popular approach particularly for studies in neuroscience. However
Dholakia, Kishan +3 more
core +2 more sources
Chemical clearing and dehydration of GFP expressing mouse brains.
PLoS ONE, 2012 Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part benzyl alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence of GFP expressing specimens, so that one has
Klaus Becker +4 more
doaj +1 more source
Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata
Bio-Protocol, 2021 The pancreas is a heavily innervated organ, but pancreatic innervation can be challenging to comprehensively assess using conventional histological methods.
Alexandra Alvarsson +6 more
doaj +1 more source
Three-dimensional microscopic analysis of clinical prostate specimens [PDF]
, 2016 __Aims:__ Microscopic evaluation of prostate specimens for both clinical and research purposes is generally performed on 5-μm-thick tissue sections.
Cappellen, W.A. (Gert) van +6 more
core +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT Background B‐cell lymphoblastic lymphoma (B‐LBL) represents a rare variety of non‐Hodgkin lymphoma, with limited research on its biology, progression, and management. Methods A retrospective analysis was performed on the clinical characteristics of 256 patients aged ≤18 years who received treatment under the China Net Childhood Lymphoma (CNCL)‐
Zhijuan Liu +20 more
wiley +1 more source