Results 81 to 90 of about 2,173,418 (363)

Isolation and primary cultures of human intrahepatic bile ductular epithelium [PDF]

, 1988
A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described.
A. E. Sirica, A. G. Grant, A. J. Demetris, A. J. Demetris, A. J. Demetris, B. H. Markus, C. Petropoulos, D. C. Snover, H. O. Jauregni, H. Yoshimura, J. J. Fung, J. W. Grisham, J. W. Grisham, J. W. Grisham, L. M. Ried, L. Makowka, N. Fausto, N. T. Hayner, R. Duquesnoy, R. N. M. MacSween, S. Graner, S. L. Hammond, S. Saidman, T. E. Starzl, T. Ming-Sound   +24 more
core   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. [PDF]

, 1992
We are developing efficient methods for gene transfer into tissue culture cells. We have previously shown that coupling of a chimeric adenovirus with polylysine allowed the construction of an adenovirus-polylysine-reporter-gene complex that transferred ...
Birnstiel, Max L., Cotten, Matt, Curiel, David T., Kirlappos, Helen, Mechtler, Karl, Wagner, Ernst, Zatloukal, Kurt   +6 more
core   +1 more source

The propagation of ?coronaviruses? in tissue-culture [PDF]

Archiv f�r die gesamte Virusforschung, 1969
The conditions for the cultivation of the 229-E respiratory virus in tissue culture are described. The virus multiplies only in human tissue culture systems, but a variety of cell types are sensitive to it. The virus has been isolated in three continuous cell lines and a plaque assay has been developed in one of these.
D. A. J. Tyrrell, A. F. Bradburne
openaire   +3 more sources

Redox‐dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells

FEBS Letters, EarlyView.
In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker, Nimit Mistry, Nicoletta Plotegher, Elizabeth R. Westbrook, Michael D. E. Sewell, John Carroll, Gyorgy Szabadkai, Angus J. Bain, Michael R. Duchen   +8 more
wiley   +1 more source

In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate

Journal of Genetic Engineering and Biotechnology, 2020
Background Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity.
Heba El-Sayed Ghareb, Shafik Darwish Ibrahim, Ghada Abd El-Moneim Hegazi   +2 more
doaj   +1 more source

MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

Molecular Oncology, EarlyView.
MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin, Audrey Vinchent, Marie Fernandes, Isabelle Damour, Agathe Laratte, Rémi Tellier, Gabriella O. Estevam, Jean‐Pascal Meneboo, Céline Villenet, Clotilde Descarpentries, James S. Fraser, Martin Figeac, Alexis B. Cortot, Etienne Rouleau, David Tulasne   +14 more
wiley   +1 more source

Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair [PDF]

, 2013
Tubular scaffolds which incorporate a variety of micro- and nanotopographies have a wide application potential in tissue engineering especially for the repair of spinal cord injury (SCI).
Bereiter-Hahn J., Casey B. G., Cavaglia M., Chary S. R., Clark P., Csaderova L., Gadegaard N., Gadegaard N., Gailit J., Gilbert R. J., Huh D., Lakatos A., Mathis O. Riehle, Mirastschijski U., Nikolaj Gadegaard, Noble M., Peter S. Donoghue, Robert P., Ruff R. L., Salem A. K., Sarkar S., Seunarine K., Sun T., Sun T., Sun T., Susan C. Barnett, Sørensen A., Sørensen A., Tao Sun, Thomson C. E., Van Harreveld A., Yamada M.   +31 more
core   +3 more sources

Computational fluid dynamic analysis of bioprinted self-supporting perfused tissue models [PDF]

, 2020
Natural tissues are incorporated with vasculature, which is further integrated with a cardiovascular system responsible for driving perfusion of nutrient‐rich oxygenated blood through the vasculature to support cell metabolism within most cell‐dense ...
Bonewald, Lynda F., Ekser, Burcin, Li, Ping, McCarthy, Brian Paul, Prideaux, Matthew, Sego, T. J., Smith, Lester Jeshua, Sterner, Jane, Tovar, Andres   +8 more
core   +1 more source

Peripheral blood proteome biomarkers distinguish immunosuppressive features of cancer progression

Molecular Oncology, EarlyView.
Immune status significantly influences cancer progression. This study used plasma proteomics to analyze benign 67NR and malignant 4T1 breast tumor models at early and late tumor stages. Immune‐related proteins–osteopontin (Spp1), lactotransferrin (Ltf), calreticulin (Calr) and peroxiredoxin 2 (Prdx2)–were associated with systemic myeloid‐derived ...
Yeon Ji Park, Jae Won Oh, Hyewon Chung, Jung Won Kwon, Yi Rang Na, Kwang Pyo Kim, Seung Hyeok Seok   +6 more
wiley   +1 more source