Results 201 to 210 of about 781,157 (254)

Techniques for Mammalian Cell Tissue Culture

Current Protocols in Human Genetics, 1994
AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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Tissue Culture Techniques

1982
Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
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Overview of Cell and Tissue Culture Techniques

Current Protocols in Pharmacology, 1999
AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
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Basic Techniques in Mammalian Cell Tissue Culture

Current Protocols in Cell Biology, 2007
AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
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Plant tissue culture techniques—Tools in plant micropropagation

Current Opinion in Biotechnology, 2011
European Biotechnology Congress -- SEP 28-OCT 01, 2011 -- Istanbul ...
Onay, Ahmet, Yildirim, Hakan, Tokatli, Yelda Ozden, Akdemir, Hulya, Suzerer, Veysel   +4 more
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Plant Tissue Culture Techniques for Breeding

2018
Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been applied successfully in many species and have been used to create plants that are disease and insect resistant and tolerant to water stress and demonstrate enhanced growth and improved yields and much more.
Mark P. Bridgen, Wouter Van Houtven, Tom Eeckhaut   +2 more
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Techniques for Selecting Mutants from Plant Tissue Cultures

2003
The theory and goals of mutant selection from plant tissue cultures have been reviewed extensively over the past few years (I-5), and will only be dealt with in a cursory manner in this chapter. For more detail, the reader is encouraged to consult these review articles. The ideal plant tissue culture system is useful for mutation selection because it:
D R, Duncan, J M, Widholm
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Clonal propagation of Callistemon by tissue culture techniques

Plant Cell Reports, 1982
Callus development in Callistemon viminalis was readily achieved when axillary buds derived from nodal tissue were placed in a medium containing macro- and micro-nutrients, sucrose (0.06 M), inositol (300 μM), nicotinic acid (20 μM), pyridoxine hydrochloride (3 μM), thiamine hydrochloride (2 μM), riboflavin (10 μM), cytokinins (5 μM) and auxins (0.1 μM)
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Plant Tissue Culture Techniques and its Achievements

2018
Some of the simpler techniques that are more approachable and have been found to be applied directly in plant propagation and genetic improvement of plants are micropropagation, meristem culture, somatic embryogenesis, somaclonal variation, embryo culture, in vitro selection, anther culture, and protoplast culture.
S. Pandey, V. C. Pandey
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Tissue Culture Techniques

American Journal of Ophthalmology, 1955
Frances Doane, A.J. Rhodes, H.L. Ormsby
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