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Membrane Extracts from Plant Tissues
2020The comparison of isolated plant cell membranous enclosures can be hampered if their extraction method differs, e.g., in regard to the utilized buffers, the tissue, or the developmental stage of the plant. Thus, for comparable results, different cellular compartments should be isolated synchronously in one procedure.
Uwe, Bodensohn +3 more
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Extracting insights from heterogeneous tissues
Nature Computational Science, 2021A limitation of data obtained from RNA-seq experiments is the presence of different types of cell expression, making it difficult to identify the contribution of cell-type composition or cell-type-specific expression. A new study addresses this problem by proposing a method for cell-type-aware analysis of RNA-seq data.
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Glycogenolytic effect of adipose tissue extract
Experientia, 1975L'administration i.v. d'un extrait aqueux de tissu adipeux (ATE) a des rats intacts surrenalectomises provoque un etat de glycogenolyse hepatique suivi d'hypoglycemie. La glycogenolyse semble etre independante de l'epinephrine. L'hypoglycemie provoquee par l'ATE resulte en partie de la mobilisation acceleree des reserves de glycogene hepatique.
B W, Siegel, T R, Blohm, N L, Wiech
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American Journal of Enology and Viticulture, 1990
A simple chromatographic method using Sephadex LH-20 was developed to separate non-polymeric and polymeric phenols. Non-polymeric phenols are eluted from the gel by 60% methanol.
Kent Kantz, V. L. Singleton
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A simple chromatographic method using Sephadex LH-20 was developed to separate non-polymeric and polymeric phenols. Non-polymeric phenols are eluted from the gel by 60% methanol.
Kent Kantz, V. L. Singleton
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Protoheme extraction from plant tissue
Analytical Biochemistry, 1978A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether.
L C, Stillman, M L, Gassman
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Radioimmunological Determination of Cholecystokinin in Tissue Extracts
Digestion, 1982A sensitive and precise radioimmunoassay has been developed. Cholecystokinin (CCK) was labeled by the Bolton-Hunter method. The antibody bound CCK8 and CCK33. The binding sites of the antibody are located by the sulfated tyrosil group in position 27 of the CCK molecule. Human duodenum tissue contains larger forms of CCK33 as well as CCK33 and CCK8.
A, Schafmayer, M, Werner, H D, Becker
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NMR Spectroscopy of Biofluids, Tissues, and Tissue Extracts
1996Metabonomics is the comprehensive and simultaneous profiling of multiple metabolite levels in a biological system and their temporal changes caused by factors such as diet, lifestyle, environment, genetic factors, and pharmacological effects. This is usually carried out by investigating the metabolic content of biofluids and tissues using techniques ...
John C. Lindon, Elaine Holmes
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Simultaneous Extraction of Tissue Norepinephrine and Serotonin
Journal of Pharmaceutical Sciences, 1969A new procedure for the extraction of tissue monoamines combines the homogenization and butanol extraction steps. In addition to the significant reduction in the time necessary for extraction which is characteristic of single-extraction procedures, greater extraction efficiencies are obtained.
B M, Phillips +3 more
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Extraction and quantification of daunomycin and doxorubicin in tissues
Journal of Chromatography B: Biomedical Sciences and Applications, 1980The measurement of intracellular concentrations of the anti-cancer drug doxorubicin was performed by the application of a simple cell extraction technique combined with a rapid high-performance liquid chromatographic separation. Quantitation was done by fluorescence detection.
J F, Strauss +3 more
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THE EXTRACTABLE HEPARIN IN DIFFERENT ANIMAL TISSUE
Canadian Journal of Biochemistry and Physiology, 1956Heparin is extracted from tissue by 0.5 M potassium thiocyanate. The protein is removed from the extract with 80% phenol and the heparin precipitated from the aqueous layer with three volumes of 95% ethanol. Further purification is achieved by reprecipitating the heparin with alcohol, dissolving the precipitate in saline, and precipitating it with 5 ...
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