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Extraction of Proteins from Plant Tissues
Current Protocols in Protein Science, 2004AbstractA basic protocol is described for extracting protein from plants. Suggestions are included for overcoming some of the common obstacles encountered (e.g., acidity, phenolics, cell walls).
William, Laing, John, Christeller
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Carotenoid Extraction from Plant Tissues
Carotenoids are the natural pigments available in nature and exhibit different colors such as yellow, red, and orange. These are a class of phytonutrients that have anti-cancer, anti-inflammatory, anti-oxidant, immune-modulatory, and anti-aging properties. These were used in food, pharmaceutical, nutraceutical, and cosmetic industries. They are dividedKagolla, Priscilla +6 more
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Protein Extraction from Solid Tissue
2010Maximal extraction and solubilization of protein from diseased or healthy tissue is important to make the whole protein complement available for proteomic analysis. It also helps to maximize reproducibility and to minimize waste. Minimal degradation of the protein amino acid backbone or dephosphorylation is essential to preserve the analytical utility ...
Christer, Ericsson, Monica, Nistér
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Extraction of RNA From Cells and Tissue
2005Total undegraded cellular RNA, largely free of contaminating DNA, can be rapidly and easily isolated from homogenized tissue or cultured cells using acid pH guanidinium thiocyanate/phenol/chloroform extraction. Commercially available acid phenol/guanidinium thiocyanate solutions allow one-step complete solubilization of tissue or cells under conditions
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DNA extraction from plant tissue.
2008This chapter discusses the approaches that have been used in the isolation of DNA from plant tissues. A summary of approaches is presented in the appendix.
Kasem, S., Rice, N., Henry, R. J.
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Spasmolytic Effect of Cerebral Tissue Extracts
Nature, 1956USING isolated segments of the guinea pig intestine suspended in oxygenated Tyrode solution at 35° C., strong contractions induced by acetylcholine and adenosine triphosphate were relaxed by introducing concentrated extracts of rat brain in Tyrode solution1.
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Estimation of the blood contamination of tissue extracts
Analytical Biochemistry, 1983A method based on measurement of absorbance at 540 or 580 nm is described. The results obtained were closely correlated to the actual level of blood present in different tissues, as determined by use of radiolabeled albumin. The present technique is much simpler than earlier methods used to determine the degree of blood contamination of tissue extracts.
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