Results 271 to 280 of about 418,065 (313)

Extracting insights from heterogeneous tissues

Nature Computational Science, 2021
A limitation of data obtained from RNA-seq experiments is the presence of different types of cell expression, making it difficult to identify the contribution of cell-type composition or cell-type-specific expression. A new study addresses this problem by proposing a method for cell-type-aware analysis of RNA-seq data.
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NMR Spectroscopy of Biofluids, Tissues, and Tissue Extracts

1996
Metabonomics is the comprehensive and simultaneous profiling of multiple metabolite levels in a biological system and their temporal changes caused by factors such as diet, lifestyle, environment, genetic factors, and pharmacological effects. This is usually carried out by investigating the metabolic content of biofluids and tissues using techniques ...
John C. Lindon, Elaine Holmes
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Simultaneous Extraction of Tissue Norepinephrine and Serotonin

Journal of Pharmaceutical Sciences, 1969
A new procedure for the extraction of tissue monoamines combines the homogenization and butanol extraction steps. In addition to the significant reduction in the time necessary for extraction which is characteristic of single-extraction procedures, greater extraction efficiencies are obtained.
B M, Phillips, P J, Kraus, T L, Hammes, J L, Leeling   +3 more
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Extraction and quantification of daunomycin and doxorubicin in tissues

Journal of Chromatography B: Biomedical Sciences and Applications, 1980
The measurement of intracellular concentrations of the anti-cancer drug doxorubicin was performed by the application of a simple cell extraction technique combined with a rapid high-performance liquid chromatographic separation. Quantitation was done by fluorescence detection.
J F, Strauss, R L, Kitchens, V W, Patrizi, E P, Frenkel   +3 more
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Protoheme extraction from plant tissue

Analytical Biochemistry, 1978
A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether.
L C, Stillman, M L, Gassman
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Radioimmunological Determination of Cholecystokinin in Tissue Extracts

Digestion, 2009
A sensitive and precise radioimmunoassay has been developed. Cholecystokinin (CCK) was labeled by the Bolton-Hunter method. The antibody bound CCK₈ and CCK₃₃. The binding sites of the antibody are located by the sulfated tyrosil group in position 27 of the CCK molecule. Human duodenum tissue contains larger
A, Schafmayer, M, Werner, H D, Becker
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Extraction of Proteins from Plant Tissues

Current Protocols in Protein Science, 2004
AbstractA basic protocol is described for extracting protein from plants. Suggestions are included for overcoming some of the common obstacles encountered (e.g., acidity, phenolics, cell walls).
William, Laing, John, Christeller
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Carotenoid Extraction from Plant Tissues

Carotenoids are the natural pigments available in nature and exhibit different colors such as yellow, red, and orange. These are a class of phytonutrients that have anti-cancer, anti-inflammatory, anti-oxidant, immune-modulatory, and anti-aging properties. These were used in food, pharmaceutical, nutraceutical, and cosmetic industries. They are divided
Kagolla, Priscilla, Vinay, Sharma, Ashish, Gautam, Prateek, Gupta, Rinku, Dagar, Vimal, Kishore, Rakesh, Kumar   +6 more
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THE EXTRACTABLE HEPARIN IN DIFFERENT ANIMAL TISSUE

Canadian Journal of Biochemistry and Physiology, 1956
Heparin is extracted from tissue by 0.5 M potassium thiocyanate. The protein is removed from the extract with 80% phenol and the heparin precipitated from the aqueous layer with three volumes of 95% ethanol. Further purification is achieved by reprecipitating the heparin with alcohol, dissolving the precipitate in saline, and precipitating it with 5 ...
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Protein Extraction from Solid Tissue

2010
Maximal extraction and solubilization of protein from diseased or healthy tissue is important to make the whole protein complement available for proteomic analysis. It also helps to maximize reproducibility and to minimize waste. Minimal degradation of the protein amino acid backbone or dephosphorylation is essential to preserve the analytical utility ...
Christer, Ericsson, Monica, Nistér
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