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Extraction of RNA From Cells and Tissue
2005Total undegraded cellular RNA, largely free of contaminating DNA, can be rapidly and easily isolated from homogenized tissue or cultured cells using acid pH guanidinium thiocyanate/phenol/chloroform extraction. Commercially available acid phenol/guanidinium thiocyanate solutions allow one-step complete solubilization of tissue or cells under conditions
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DNA extraction from plant tissue.
2008This chapter discusses the approaches that have been used in the isolation of DNA from plant tissues. A summary of approaches is presented in the appendix.
Kasem, S., Rice, N., Henry, R. J.
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Spasmolytic Effect of Cerebral Tissue Extracts
Nature, 1956USING isolated segments of the guinea pig intestine suspended in oxygenated Tyrode solution at 35° C., strong contractions induced by acetylcholine and adenosine triphosphate were relaxed by introducing concentrated extracts of rat brain in Tyrode solution1.
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Estimation of the blood contamination of tissue extracts
Analytical Biochemistry, 1983A method based on measurement of absorbance at 540 or 580 nm is described. The results obtained were closely correlated to the actual level of blood present in different tissues, as determined by use of radiolabeled albumin. The present technique is much simpler than earlier methods used to determine the degree of blood contamination of tissue extracts.
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The Extraction of Biotin from Tissues
Science, 1941R C, Thompson, R E, Eakin, R J, Williams
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Protein Extraction From Mammalian Tissues
2007For Western blotting and coimmunoprecipitation (coIP), protein samples must be extracted from tissues. The protocol described in this chapter has been used to extract clock proteins from mammalian tissues as diverse as liver, kidney, and brain. The extraction protocol is mild enough to be used for coIP as well as Western blotting.
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Sex differences in the pharmacokinetics and tissue residues of Macleaya cordata extracts in rats
Xenobiotica, 2022Zhi-Liang Sun
exaly

