Results 151 to 160 of about 7,251 (175)
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Role of the Klebsiella pneumoniae TolC porin in antibiotic efflux
Research in Microbiology, 2019The major Gram-negative gated efflux channel TolC has been extensively characterized in Escherichia coli but there is minimal information about Klebsiella pneumoniae TolC. Using an arabinose-inducible plasmid-based expression system, we show that the K. pneumoniae TolC complements the efflux defect in an E.
Ramkumar Iyer +2 more
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Functional Analysis of TolC Homologs in Vibrio vulnificus
Current Microbiology, 2014Gram-negative bacteria use tripartite pumps to transport antibacterial drugs and other toxic compounds across the inner and outer membranes, which are separated by the periplasmic space. The TolC protein is an outer membrane factor that participates in the formation of tripartite efflux pumps. The genome of Vibrio vulnificus encodes two E.
Seunghwa, Lee +2 more
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TolC of Escherichia coli Functions as an Outer Membrane Channel
Zentralblatt für Bakteriologie, 1993Reconstitution experiments were performed with TolC from Escherichia coli outer membrane by using the lipid bilayer membrane technique. TolC was purified by elution of the oligomeric and the monomeric forms out of preparative SDS-PAGE. The oligomeric but not the monomeric form of the protein was able to increase the specific conductance of artificial ...
R, Benz, E, Maier, I, Gentschev
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Structures and Efflux Mechanisms of the AcrAB-TolC Pump
The global emergence of multidrug resistance (MDR) in gram-negative bacteria has become a matter of worldwide concern. MDR in these pathogens is closely linked to the overexpression of certain efflux pumps, particularly the resistance-nodulation-cell division (RND) efflux pumps. Inhibition of these pumps presents an attractive and promising strategy toZhili, Yu, Xiaodong, Shi, Zhao, Wang
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Electrophysiological Behavior of the TolC Channel-Tunnel in Planar Lipid Bilayers
Journal of Membrane Biology, 2002Escherichia coli TolC assembles into the unique channel-tunnel structure spanning the outer membrane and periplasmic space. The structure is constricted only at the periplasmic entrance of the tunnel and this must be opened to allow export of substrates bound by cognate inner membrane complexes. We have investigated the electrophysiological behavior of
C, Andersen, C, Hughes, V, Koronakis
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Structure and Function of TolC: The Bacterial Exit Duct for Proteins and Drugs
Annual Review of Biochemistry, 2004▪ Abstract The bacterial TolC protein plays a common role in the expulsion of diverse molecules, which include protein toxins and antibacterial drugs, from the cell. TolC is a trimeric 12-stranded α/β barrel, comprising an α-helical trans-periplasmic tunnel embedded in the outer membrane by a contiguous β-barrel channel.
Vassilis, Koronakis +2 more
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Stoichiometry and mechanistic implications of the MacAB-TolC tripartite efflux pump
Biochemical and Biophysical Research Communications, 2017The MacAB-TolC tripartite efflux pump is involved in resistance to macrolide antibiotics and secretion of protein toxins in many Gram-negative bacteria. The pump spans the entire cell envelope and operates by expelling substances to extracellular space.
Inseong Jo +8 more
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Efflux of antibiotics by the homotrimeric TolC transmembrane protein
Biophysical Journal, 2023Isik Kantarcioglu +4 more
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In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution
Structure, 2022Muyuan Chen, Xiaodong Shi, Zhili Yu
exaly
Structural characterization of the EmrAB-TolC efflux complex from E. coli
Biochimica Et Biophysica Acta - Biomembranes, 2021Laetitia Daury +2 more
exaly