Results 101 to 110 of about 16,582,220 (297)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

E-Beam Irradiation and Ozonation as an Alternative to the Sulphuric Method of Wine Preservation

Molecules, 2019
Potassium metabisulphite is usually used for microbial stabilization in the process of vinification and wine preservation, but it is considered to be allergenic.
Magdalena Błaszak, Agata Nowak, Sabina Lachowicz, Wojciech Migdał, Ireneusz Ochmian   +4 more
doaj   +1 more source

Assessment of humoral immunity in workers occupationally exposed to low levels of ionizing radiation [PDF]

Background: The aim of this study was investigating the effect of low levels of ionizing radiation on immunoglobulin, complement levels in radiology workers occupationally exposed to ionizing radiation.
رفاهی, سهیلا, رجائی اسكوئی, مهرانگیز, پورعیسی, مسعود, تبرائی, یاسر   +3 more
core  

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Association between occupational ionizing radiation exposure duration and the increased risk of dyslipidemia: evidence from a large group of radiation workers

Frontiers in Public Health
BackgroundAlthough radiation workers’ exposure levels consistently remained below established safety thresholds, accumulating evidence demonstrates that chronic low-dose ionizing radiation exposure may still pose significant health risks to humans.
Meimei Zhong, Meimei Zhong, Xiaowen Wang, Qifeng Li, Zhijia Wu, Zhijia Wu, Xiangyuan Huang, Xiangyuan Huang, Xinyue Li, Xinyue Li, Yiqing Lian, Yingyi Peng, Zhiqiang Li, Zhiqiang Li, Zhifang Liu, Qia Wang, Yajun Gong, Yajun Gong, Peixia Hu, Peixia Hu, Xiaoyong Liu, Shuming Zhu, Shaomin Wu, Fangfang Zeng, Yiru Qin, Qiying Nong, Jinhan Wang, Yeqing Gu, Yongshun Huang, Wangjian Zhang, Na Zhao, Na Zhao, Na Zhao   +32 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

NASA Goddard Space Flight Center's Compendium of Total Ionizing Dose, Displacement Damage Dose, and Single-Event Effects Test Results

, 2019
Total ionizing dose, displacement damage dose, and single-event effect testing were performed to characterize and determine the suitability of candidate electronics for NASA space utilization.
Wilcox, Edward P., Topper, Alyson D., Casey, Megan C., Cochran, Donna J., Wyrwas, Edward J., Pellish, Jonathan A., Stansberry, Scott D., Ryder, Kaitlyn, O'Bryan, Martha V., Ladbury, Ray L., Campola, Michael J., Majewicz, Peter J., Lauenstein, Jean-Marie, Berg, Melanie D., LaBel, Kenneth A.   +14 more
core  

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source