Results 41 to 50 of about 3,562,641 (311)

Comparison of the major cell populations among osteoarthritis, Kashin–Beck disease and healthy chondrocytes by single-cell RNA-seq analysis

Cell Death and Disease, 2021
Chondrocytes are the key target cells of the cartilage degeneration that occurs in Kashin–Beck disease (KBD) and osteoarthritis (OA). However, the heterogeneity of articular cartilage cell types present in KBD and OA patients and healthy controls is ...
Xi Wang, Yujie Ning, Pan Zhang, Blandine Poulet, Ruitian Huang, Yi Gong, Minhan Hu, Cheng Li, Rong Zhou, Mikko J. Lammi, Xiong Guo   +10 more
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Assessing particle count in electron microscopy measurements of nanomaterials to support regulatory guidance

Scientific Reports
In the European Union, nanomaterials are regulated through different pieces of sectoral legislation. This legislation often requires risk assessments and thus reliable characterization data, for which regulatory guidance generally recommend electron ...
Charlotte Wouters, Vikram Kestens, Eveline Verleysen, Jan Mast   +3 more
doaj   +1 more source

Impact of soil-type, soil-pH, and soil-metal (loids) on grain-As and Cd accumulation in Malawian rice grown in three regions of Malawi

Environmental Advances, 2022
Impact of soil type, soil-metal(loids) and soil-pH of rice paddies on grain-arsenic (As) and cadmium (Cd) accumulation in Malawian rice was investigated to explore the extent to which Malawi's geographical location, soil types, soil-metal(loids) and soil-
Angstone Thembachako Mlangeni, Shaun Thomas Lancaster, Andrea Raab, Eva M. Krupp, Gareth J. Norton, Joerg Feldmann   +5 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Mendelian randomization study of the relationship between blood and urine biomarkers and schizophrenia in the UK Biobank cohort

Communications Medicine
Background The identification of suitable biomarkers is of crucial clinical importance for the early diagnosis of treatment-resistant schizophrenia (TRS).
Bolun Cheng, Yunfeng Bai, Li Liu, Peilin Meng, Shiqiang Cheng, Xuena Yang, Chuyu Pan, Wenming Wei, Huan Liu, Yumeng Jia, Yan Wen, Feng Zhang   +11 more
doaj   +1 more source

The role of zinc homeostasis in major depressive disorder: heterogeneous pathological mechanisms and therapeutic implications

Annals of Medicine
Background Major depressive disorder (MDD) involves multifaceted pathologies including neurotransmission, neuroplasticity, inflammation, and hypothalamic-pituitary-adrenal (HPA) axis dysfunction.
Yuping Li, Yucui Lu, Xianmei Lin, Lichun Zhao   +3 more
doaj   +1 more source

A large-scale polygenic risk score analysis identified candidate proteins associated with anxiety, depression and neuroticism

Molecular Brain, 2022
Psychiatric disorders and neuroticism are closely associated with central nervous system, whose proper functioning depends on efficient protein renewal. This study aims to systematically analyze the association between anxiety / depression / neuroticism ...
Bolun Cheng, Xuena Yang, Shiqiang Cheng, Chun’e Li, Huijie Zhang, Li Liu, Peilin Meng, Yumeng Jia, Yan Wen, Feng Zhang   +9 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source