Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
Molecular & Cellular Oncology, 2021 R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome.
Yaqun Liu +3 more
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The Mechanism of Intrinsic Transcription Termination [PDF]
Molecular Cell, 1999 In bacteria, an intrinsic transcription termination signal appears in RNA as a hairpin followed by approximately eight uridines (U stretch) at the 3' terminus. This signal leads to rapid dissociation of the ternary elongation complex (TEC) into RNA, DNA, and an RNA polymerase.
Gusarov, Ivan, Nudler, Evgeny
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Cell Reports, 2019 Summary: In eukaryotic genomes, heterochromatin is targeted by RNAi machinery and/or by pathways requiring RNA elimination and transcription termination factors.
Tommy V. Vo +8 more
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Enhancement of Transcription by a Splicing-Competent Intron Is Dependent on Promoter Directionality. [PDF]
PLoS Genetics, 2016 Enhancement of transcription by a splicing-competent intron is an evolutionarily conserved feature among eukaryotes. The molecular mechanism underlying the phenomenon, however, is not entirely clear. Here we show that the intron is an important regulator
Neha Agarwal, Athar Ansari
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Engineering efficient termination of bacteriophage T7 RNA polymerase transcription
G3: Genes, Genomes, Genetics, 2022 The bacteriophage T7 expression system is one of the most prominent transcription systems used in biotechnology and molecular-level research. However, T7 RNA polymerase is prone to read-through transcription due to its high processivity. As a consequence,
Diana G Calvopina-Chavez +2 more
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Experimental characterization of Cis-acting elements important for translation and transcription in Halophilic Archaea [PDF]
, 2007 Summary The basal transcription apparatus of archaea is well characterized. However, much less is known about the mechanisms of transcription termination and translation initation.
Jörg Soppa +11 more
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Topoisomerases, chromatin and transcription termination [PDF]
Transcription, 2011 In eukaryotes transcription is complicated by the DNA being packed in nucleosomes and by supercoils induced by opening of the DNA double helix during elongation. Here we discuss our recent genome-wide work regarding topoisomerases and their role in chromatin remodeling during the transcription cycle and we report a novel function for topoisomerases in ...
Mickaël, Durand-Dubief +3 more
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Cellular mRNAs access second ORFs using a novel amino acid sequence-dependent coupled translation termination-reinitiation mechanism [PDF]
, 2014 Polycistronic transcripts are considered rare in the human genome. Initiation of translation of internal ORFs of eukaryotic genes has been shown to use either leaky scanning or highly structured IRES regions to access initiation codons.
Dyer, Nigel P. +4 more
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Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast
Scientific Reports, 2017 In budding yeast, the 3′ end processing of mRNA and the coupled termination of transcription by RNAPII requires the CF IA complex. We have earlier demonstrated a role for the Clp1 subunit of this complex in termination and promoter-associated ...
Nadra Al-Husini +4 more
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Maintenance of Transcription-Translation Coupling by Elongation Factor P
mBio, 2016 Under conditions of tight coupling between translation and transcription, the ribosome enables synthesis of full-length mRNAs by preventing both formation of intrinsic terminator hairpins and loading of the transcription termination factor Rho.
Sara Elgamal +2 more
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