Results 91 to 100 of about 4,769,934 (397)

Designing Lentiviral Vectors for Gene Therapy of Genetic Diseases

Viruses, 2021
Lentiviral vectors are the most frequently used tool to stably transfer and express genes in the context of gene therapy for monogenic diseases. The vast majority of clinical applications involves an ex vivo modality whereby lentiviral vectors are used ...
Valentina Poletti, Fulvio Mavilio
doaj   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

Connexins: synthesis, post-translational modifications, and trafficking in health and disease [PDF]

, 2018
Connexins are tetraspan transmembrane proteins that form gap junctions and facilitate direct intercellular communication, a critical feature for the development, function, and homeostasis of tissues and organs.
Aasen, Trond, Johnstone, Scott, Koval, Michael, Lynn, K. Sabrina, Universitat Autònoma de Barcelona, Vidal-Brime, Laia   +5 more
core   +3 more sources

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head

Nature Communications
To shed light on the enigmatic origin of the vertebrate head, our study employs an integrated approach that combines single-cell transcriptomics, perturbations in signaling pathways, and cis-regulatory analysis in amphioxus.
Anna Markos, Jan Kubovciak, Simona Mikula Mrstakova, Anna Zitova, Jan Paces, Simona Machacova, Zbynek Kozmik-Jr, Zbynek Kozmik, Iryna Kozmikova   +8 more
doaj   +1 more source

The lower bound on the precision of transcriptional regulation [PDF]

, 2014
The diffusive arrival of transcription factors at the promoter sites on the DNA sets a lower bound on how accurately a cell can regulate its protein levels.
Paijmans, Joris, Wolde, Pieter Rein ten
core   +2 more sources

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

LncRNA EPR controls epithelial proliferation by coordinating Cdkn1a transcription and mRNA decay response to TGF-β

Nature Communications, 2019
Several lncRNAs are regulated by TGF-β. Here the authors report that an intergenic lncRNA —EPR— is a component of the TGF-β signaling pathway and controls epithelial cell proliferation by altering transcription and mRNA decay of Cdkn1a.
Martina Rossi, Gabriele Bucci, Dario Rizzotto, Domenico Bordo, Matteo J. Marzi, Margherita Puppo, Arielle Flinois, Domenica Spadaro, Sandra Citi, Laura Emionite, Michele Cilli, Francesco Nicassio, Alberto Inga, Paola Briata, Roberto Gherzi   +14 more
doaj   +1 more source

NLRX1 Deficiency Alters the Gut Microbiome and Is Further Exacerbated by Adherence to a Gluten-Free Diet

Frontiers in Immunology, 2022
Patients with gluten sensitivities present with dysbiosis of the gut microbiome that is further exacerbated by a strict adherence to a gluten-free diet (GFD).
Holly A. Morrison, Yang Liu, Kristin Eden, Kristin Eden, Margaret A. Nagai-Singer, Paul A. Wade, Irving C. Allen, Irving C. Allen   +7 more
doaj   +1 more source

Roles of Non-Coding RNAs in Transcriptional Regulation

Transcriptional and Post-transcriptional Regulation, 2018
Non-coding RNAs (ncRNAs) are functional RNA molecules that are transcribed from mammalian genome but lack protein coding capacity. Nearly 80% of the human genome constitutes non-coding elements such as small non-coding RNAs, sncRNAs (miRNA, piRNA, SiRNA,
Loudu Srijyothi, S. Ponne, Talukdar Prathama, C. Ashok, S. Baluchamy   +4 more
semanticscholar   +1 more source